The Efficacy of Catfish Skin Gelatin (Pangasius djambal) on the Oral Cavity Soft Tissue Healing

Fredy Mardiyantoro*  -  (Scopus ID : 572083117580) 1. Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Universitas Brawijaya, Indonesia. 2. Department of Oral Biochemistry, Graduate School of Medical and Dental Sciences, Kagoshima University, Japan, Indonesia
Lukman Hakim Hidayat  -  Department of Oral Medicine, Faculty of Dentistry, Universitas Brawijaya, Indonesia, Indonesia
Vira Dwiviasta Ade Ayu  -  Faculty of Dentistry, Universitas Brawijaya, Indonesia, Indonesia
Asma Syahidah Illia  -  Faculty of Dentistry, Universitas Brawijaya, Indonesia, Indonesia
Diva Alenta Arzeti  -  Faculty of Dentistry, Universitas Brawijaya, Indonesia, Indonesia

(*) Corresponding Author
DOI: 10.30659/odj.13.2.106-118

Background: Traumatic ulcers are common oral mucosal lesions causing discomfort, infection, and delayed healing. Conventional therapy is commonly used, but the use of herbal materials is expected to be an affordable and effective alternative therapy. Gelatin was chosen because it is a natural biomaterial rich in amino acids, which play a role in regulating inflammation and tissue regeneration, particularly in influencing the number of macrophages and lymphocytes in the inflammatory phase and fibroblast proliferation. The purpose of the study was to determine the effect of Pangasius djambal fish skin gelatin on the healing process of traumatic ulcers in Rattus norvegicus.

Method: A Wistar rats (Rattus norvegicus) were used as research subjects. Traumatic ulcers were induced on the lower jaw labial mucosa using thermal trauma. Observations were made on days 3, 5, and 7. The number of macrophages, lymphocytes, and fibroblasts was counted using hematoxylin-eosin (HE) staining under a microscope at 400× magnification.

Result: Based on the results of the One-way ANOVA parametric test, a value of (p < 0.05) was obtained, indicating a significant difference in the number of macrophages, lymphocytes, and fibroblasts between groups. 

Conclusion: Pangasius djambal skin gelatin can effect the healing process of traumatic ulcers in Rattus norvegicus.

Keywords: Macrophage, Fibroblast, Lymphocyte, Pangasius djambal, Traumatic ulcer

  1. Sa’adah N, Hendarti H, Prehananto H, Soebadi B, Pertiwi EP, Adriansyah A. The Effect of Basil Leaves (Ocimum Sanctum L.) Extract Gel to Traumatic Ulcer Area in Rattus Norvegicus. Jurnal Kesehatan Gigi 2020;8(1).
  2. Arundina I, Diyatri I, Kusumaningsih T, Surboyo MDC, Monica E, Afanda NM. The Role of Rice Hull Liquid Smoke in the Traumatic Ulcer Healing. Eur J Dent 2021;15(1):33–38.
  3. Mulawarmanti D, Revianti S, Wahjuningsih E. Efficacy of Topical Application of Chum Salmon (Oncorhynchus keta) Skin-derived Collagen Extracts in Improving Oral Traumatic Ulcer Healing. Contemp Clin Dent 2024;15(2):124–128.
  4. Umayah M, Nurul Sidiqa A, Pendidikan Profesi Dokter Gigi P, et al. Ulser Traumatik Akibat Rotasi dan Migrasi Gigi. SONDE (Sound of Dentistry) 2021;6(2).
  5. Savitri IJ, Suprarahardiani SA, Ulinnuha LK, et al. TNF-α Expression and Plasma Cell and Macrophage Numbers in Traumatic Ulcers in a Rat Model Treated by Topical or Systemic Probiotic L. casei Shirota. Int J Med Sci 2025;22(2):318–327.
  6. Mardiyantoro F, Prasetyaningrum N, Hidayat LH, et al. Pangasius djambal gelatin increases osteoprotegerin, decreases RANK and RANKL after tooth extraction in vivo. Dent J 2023;56(4):238–242.
  7. Bindaputri JF, Sudiono J. CD68 Expression on Macrophages as Anti-Inflammatory Effect of Tamarillo (Solanum betaceum Cav.) Fruit Peel Ethanol Extract (Study on Carrageenan-Induced Buccal Mucosa of Rats). Jurnal Biomedika dan Kesehatan 2024;7(1):6–16.
  8. Willenborg S, Injarabian L, Eming SA. Role of Macrophages in Wound Healing. Cold Spring Harb Perspect Biol 2022;14(12).
  9. Mardiyantoro F, Munika K, Sutanti V, Cahyati M, Pratiwi A. Penyembuhan luka rongga mulut. Universitas Brawijaya Press, 2018;
  10. Dick MK, Miao JH, Faten ;, Affiliations L. Histology, Fibroblast. In: StatPearls [Internet]. StatPearls Publishing, 2023;
  11. Muhammad Fajrin Wijaya, Aditya H. Asmara, Indrya Kirana Mattulada, Andy Fairuz Zuraida Eva, Andi Fajrin Perdana Sam. Efektivitas Bahan Alami dalam Meningkatkan Jumlah Sel Fibroblas pada Soket Gigi Tikus Wistar Setelah Pencabutan Gigi. Corona: Jurnal Ilmu Kesehatan Umum, Psikolog, Keperawatan dan Kebidanan 2025;3(1):97–111.
  12. Triadi A. Pengaruh Gel Getah Buah Nangka (Artocarpus heterophyllus) terhadap Jumlah Fibroblas pada Proses Penyembuhan Ulser Traumatik Mukosa Labial Tikus Putih (Rattus norvegicus). Malang: 2018;
  13. Alipal J, Mohd Pu’ad NAS, Lee TC, et al. A review of gelatin: Properties, sources, process, applications, and commercialisation. In: Materials Today: Proceedings. Elsevier Ltd, 2019; p. 240–250.
  14. Effendi MC, Mardiyantoro F, Firani NK, Taufiq A, Nugraha IP. The Effect of Pangasius Djambal Gelatin Treatment on the Lymphocyte Cells after Tooth Extraction. Chiang Mai University Journal of Natural Sciences 2022;21(2).
  15. Kardikadewi VAW, Mardiyantoro F, Prasetyaningrum N, et al. Stimulation of Dental Socket Healing by Pangasius Djambal Gelatin: Evaluation of Growth Factor Expression. 2023;
  16. Warningsih T, Diharmi A, Sofyani T, et al. Pengolahan kue bolu Daun Kelor (Moringa oleifera) dan sate lilit ikan Patin (Pangasius djambal) di Desa Kuok Kecamatan Kuok Kabupaten Kampar. Seminar Nasional Pemberdayaan Masyarakat [homepage on the Internet] 2022;4:2022–2033. Available from: https://doi.org/10.31258/unricsce.4.36-42
  17. Mardiyantoro F, Fidya F, Andriani DS. Pengaruh Gelatin Ikan Patin (Pangasius Djambal) Terhadap Jumlah Fibroblas Pada Luka Pasca Pencabutan Gigi Tikus Putih (Rattus Norvegicus). ODONTO Dental Journal 2019;6(1):1–5.
  18. Nurdiani R, Prihanto AA, Firdaus M, et al. Physicochemical characteristics of Pangasius sp. skin-gelatin-based-edible film enriched with silver nanoparticles. F1000Res 2023;12:160.
  19. Suryana B, Asmawati, Fathiah, et al. Periodonsia. Purbalingga: Eureka Media Aksara, 2023;
  20. Hernawati S, Mukhdaroh V, Dewi L. The Effect of Extract of Red Pomegranate (Punica granatum Linn) on The Number of Macrophages of Ulser Traumatic of Rat Model. Health Notions [homepage on the Internet] 2023;7(2). Available from: https://heanoti.com/index.php/hnhttps://heanoti.com/index.php/hn/article/view/hn70204
  21. Setya Ayu Putri G, Muhammadiyah Semarang U, Ilmu Laboratorium Klinik M, Ilmu Keperawatan dan Kesehatan Universitas Muhammadiyah Semarang F, iPlasmed L, Ilmu Keperawatan dan Kesehatan F. GAMBARAN HISTOLOGI FASE REMODELLING JARINGAN LUKA KRONIK KULIT MENCIT SETELAH PEMBERIAN PERLAKUAN PLASMA JET. Jurnal Labora Medika 2021;5:57–62.
  22. Kurniawati A, Dewi L, Salwa A. Mechanism of Purple Leaves Extract in Reduce the Number of Lymphocytes in Traumatic Ulcer. In: Clinical Management In Dentistry-The Application Of Advanced Techniques For Dental Practice. Surabaya: Airlangga University Press, 2023; p. 44–53.
  23. Dewi AES, Sidiqa AN. Ulcer Traumaticus et Causa Trauma Occlusion. Interdental Jurnal Kedokteran Gigi (IJKG) 2024;20(2):308–312.
  24. Marques AFS, Silva NM, Cruz M da, Marques J, Mata AD da. Hyaluronic acid-based gels for oral application: Comparison of in vitro effects on gingival cells and bacteria. J Oral Biol Craniofac Res 2024;14(3):238–244.
  25. Rosada HA, Mujayanto R, Poetri AR. Ekstrak daun salam dalam meningkatkan ekspresi fibroblast growth factor pada ulkus traumatik rongga mulut. Odonto Dental Journal 2020;7(2):90–96.
  26. Savadori P, Rai PM, Tadakamadla S, et al. Minor Recurrent Aphthous Ulcer Management with Hyaluronic Acid Gel in an Italian Cohort: A Double-Blind Randomized Clinical Trial. Biomed Res Int [homepage on the Internet] 2022;2022(1):7202831. Available from: https://www.isrctn.com/ISRCTN16509838
  27. Witte SFH de, Luk F, Sierra Parraga JM, et al. Immunomodulation By Therapeutic Mesenchymal Stromal Cells (MSC) Is Triggered Through Phagocytosis of MSC By Monocytic Cells. Stem Cells 2018;36(4):602–615.
  28. Mardiyantoro F, Prasetyaningrum N, Cahyati M, Abidin ZZ, Nakamura N. Potential Effect of Djambal Catfish (Pangasius djambal) Gelatin as Biomaterial Product on Healing Socket after Tooth Extraction in Rats. Indian Journal of Forensic Medicine & Toxicology 2021;15(2):2379.

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