Datura metel SEED NANO CHITOSAN EXTRACT GEL’S EFFECT ON ANGIOGENESIS WOUND HEALING OF WISTAR RAT

Vika Asriningrum*  -  Universitas Gadjah Mada (Gadjah Mada University), Indonesia
Indra Bramanti  -  Universitas Gadjah Mada (Gadjah Mada University), Indonesia
Retno Ardhani  -  Universitas Gadjah Mada (Gadjah Mada University), Indonesia

(*) Corresponding Author
DOI: 10.30659/odj.12.3.269-279

Background: Datura metel possesses anti-inflammatory properties useful for treating oral mucosal injuries common in children, where healing relies on angiogenesis. Nano chitosan technology addresses herbal bioavailability issues. This study investigated the effect of 1% Datura metel seed nano chitosan extract gel on angiogenesis in Wistar rats.

 

Methods: Eighteen Wistar rats were divided into three groups: 1% Datura metel seed chitosan extract, 1% nano chitosan extract, and a control group. Gels were applied to mandibular gingival wounds with assessments on days 3 and 5. Histological angiogenesis counts were analyzed using two-way ANOVA (p<0.05).

 

Results: Significant differences in angiogenesis counts were found among the chitosan extract, nano chitosan extract, and positive control groups. Furthermore, variations in angiogenesis counts were observed between day 3 and day 5 across all groups.

 

Conclusion: The 1% Datura metel seed chitosan extract gel demonstrated a greater effect in triggering angiogenesis compared to the 1% nano chitosan extract and the positive control.

Keywords: angiogenesis, Datura metel seed, nano chitosan

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