EFFECTS OF CLAMSHELL (Amusium Pleuronectes) CHITOSAN EXTRACT ON THE INCREASE NUMBER OF OSTEOBLAST OF THE ALVEOLAR BONE UNDER PERIODONTITIS

Authors

  • Rama Putranto Sultan Agung Islamic University image/svg+xml
  • Muhamat Muhtar Safaat Abdurrohman Sultan Agung Islamic University image/svg+xml
  • Cantika Ofintana Grati Fakultas Kedokteran Gigi

DOI:

https://doi.org/10.30659/medali.4.3.1-5

Keywords:

Kitosan Simping ShellExtract, Periodontitis, Bone Growth, Osteoblast

Abstract

Background: Background: The number of patients with periodontal disease in Indonesia is estimated to continue to increase yearly. 4.79% or 34614 people had healthy periodontal tissue, while 95.21% or 687715 people had unhealthy periodontal tissue. Clamshell waste containing chitosan can be utilized, considering that interest in sources of raw materials derived from waste is increasing. Chitosan has a dual function, which is osteoinductive because it contains growth factors that provide growth for osteoblast cells.
Methods: A true experimental laboratory study with a post-test-only control group design was conducted on 30 Wistar rats and divided into two groups: the 0.2% chlorhexidine gluconate group and the chitosan group of scallop shells (Amusium Pleuronectes). The increase in the number of osteoblasts was compared and analyzed.
Results: The results showed that the average in the control group on days 7, 14, and 21 was 1, 3.20, 8.80, while in the treatment group on days 7, 14, and 21, it was 12.2, 21.8, and 25. Based on the One Way Anova test results, a p-value of 0,000 (p <0.05) was obtained, meaning a significant difference in the average number of osteoblast cells in each study group.
Conclusion: This study concludes that there is an increasing effect of alveolar bone osteoblasts from chitosan in scallop shell extract (Amusium Pleuronecte) in Wistar rats with periodontitis.

Author Biographies

  • Rama Putranto, Sultan Agung Islamic University
    Departemen Orthodonsia
  • Muhamat Muhtar Safaat Abdurrohman, Sultan Agung Islamic University
    Departemen Orsl Biologi
  • Cantika Ofintana Grati, Fakultas Kedokteran Gigi
    Pendidikan Dokter Gigi

References

Notohartojo IT dan Frans XSH.Gambaran Kebersihan Mulut dan Gingivitis Pada Murid Sekolah Dasar di Puskesmas Sepatan, Kabupaten Tangerang. Media Litbang Kesehatan.2010.10(4): 180-81

Badan Penelitian dan Pengembangan Kesehatan. (2013). Riset Kesehatan Dasar (RISKESDAS) 2013. Laporan Nasional 2013, 1–384. https://doi.org/1 Maret 2018

Kalghatgi Sameer, Catherine S. Spina, James C. Costello, Marc Liesa, J Ruben Morones- Ramirez, Shimyn Slomovic1, Anthony Molina, Orian S Shirihai,James J.Collins.2013. Bactericidal Antibiotics Induce Mitochondrial Dysfunction and Oxidative Damage in Mammalian Cells. Sci Transl Med. 2013 July 3; 5(192)

Abdul, G. 2014. Pemanfaatan Cangkang Kerang Hijau, Kerang Darah, dan Remis sebagai Katalis Heterogen untuk Produksi Biodiesel. Jurnal Literatur. Fak. MIPA Universitas Riau. Pekan baru.p.2-3.

Nather, A., Zameer, A., 2005. Bone Grafts And Bone Substitutes - Basic Science and Clinical Applications. World Scientific Publishing Co. Pte. Ltd

Feng, Ko H., Charles, S., AndPrashant, N.K., 2010, Novelsynthesis strategies for natural polymer and composite biomaterials as potential scaffold for tissue engineering, Phil. Trans R. Soc. A., 368, 1981-87,

Sulistiyonimgrum, R. S. 2013. Aktivasi Anti Bakteri Kiotsan dari Cangkang Simping pada Kondisi lingkungan yang berbeda: kajian pemanfaatan Limbah Kerang Simping (Amusium sp). Joernal Of Marine Research 2(4):111-117.

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Published

2022-12-12

Issue

Vol. 4: Special Issue 1. Desember 2022

Section

Articles

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