Stem Cell Mesenchymal Injection Increases Platelet-Derived Growth Factors Level and Percentage of Collagen in Third-Degree Burn injured Mice

Erna Ridawati*  -  Biomedical Sciences Department of the Medical Faculty of Sultan Agung Islamic University Jl. Kaligawe KM 4 Semarang, Central Java Indonesia, Telp. + 628 122 920 906 *Corresponding author : Email: ernarida93@yahoo.co.id, Indonesia
Taufiqurrachman Nasihun  -  Biomedical Sciences Department of the Medical Faculty of Sultan Agung Islamic University Jl. Kaligawe KM 4 Semarang, Central Java Indonesia, Telp. + 628 122 920 906, Indonesia
Wiratno Wiratno  -  Biomedical Sciences Department of the Medical Faculty of Sultan Agung Islamic University Jl. Kaligawe KM 4 Semarang, Central Java Indonesia, Telp. + 628 122 920 906, Indonesia

(*) Corresponding Author

Introduction: Mesenchymal stem cell (MSC) has been demonstrated to accelerate wound healing. Platelet-derived growth factor (PDGF) and collagen have an important role in wound healing. However, the effect of MSC on levels of PDGF and number of collagen has not been established. Objectives: To determine the effect of MSC on the levels of PDGF and the percentage of collagen in the burns healing.

Methods: in this experimental study, 20 BALB /c mice were divided into 4 groups: a control group (G-0) injected with 2 ml of distilled water and group 1 (G-1), Group 2 (G-2), and group 3 (G- 3) injected with MSC at the dose of 1 x 104, 2 x 104, and 4 x 104 respectively. The burn wound was made by attaching a metal nail that had been heated for 20 seconds at the feet of mice. Levels of PDGF on day 2 (PDGF-2) and 7 (PDGF-7) was assessed by ELISA, while on day 10 excision of skin was done to calculate the percentage of collagen.

Results: There was a significant difference in the levels of PDGF-2, PDGF-7, and the percentage of collagen ( P <0.05). Posthoc analysis showed that the levels of PDGF-2, PDGF-7, and percentage of collagen in the G-2 and G-3 was significantly higher than that of G-0, p <0.05. While the levels of PDGF-2, PDGF-7, and the percentage of collagen in the G-3 was significantly higher than that of G-2 and G-1 (p <0.05).

Conclusion: Injection of MSC at a dose of 2 x 1044 and 4 x 1044 increases the levels of PDGF and the percentage of collagen.

Keywords: Mesenchymal stem cells; PDGF; Collagen.

  1. Amable RP, V.Marcus , Teixeira T, Vieira Carias RB, Granjeiro JM, Borojevic R, 2014, Protein synthesis and secretion in human mesenchymal cells derived from bone marrow, adipose tissue and Wharton’s jelly.Cited on 16 April 2014.
  2. Ansari Mohd Matin, TR Sreekumar, Candra Vikash, Dubey Pawan, Kumar Say, Amarpal, Sharma GT, 2013, Therapeutic Potential of Canine Bone Marrow Derived Mesenchymal Stem Cells and its Conditioned Media in Diabetic Rat Wound Healing. Cited on 19 Juli 2013.
  3. Gärtner Andrea, Pereira T, Gomes R, Lúcia Luís A, França ML, Geuna S, Armada-da-Silva and M Ana Colette, 2013, Mesenchymal Stem Cells from Extra Embryonic Tissues for Tissue Engineering-Regeneration of the Peripheral Nerve. Cited on 27 Maret 2013.
  4. Kabashima Kenji, 2010, Wnt signaling is involved in the development of pachydermoperiostosis. Cited on 2 Pebruari 2010.
  5. Karimi, A. Soudman, Z.Soruji, E Taghiabadi ,SJ Mousavi. Burn, 2015, Wound Healing With Injection of Adipose-Derived Stem Cells: A Mouse Model Study.March.. Cited on 31 Maret 2015.
  6. Kuroda K, Kuang S, Taketo MM, Rudnicki MA, 2013, Canonical Wnt signaling induces BMP-4 to specify slow myofibrogenesis of fetal myoblasts. Cited on 5 Maret 2013.
  7. Liu Lingying, Yu Yonghui, Hou Yusen, Cha Jiake, 2014, Human Umbilical Cord Mesenchymal Stem Cell Transplantation Promotes Cutaneous Wound Healing of Severe Burned Rats. Cited on 20 Pebruari 2014.
  8. Li Wang, Ye Bo, Cai Xiao-Yan, 2014, Differentiation of Human Umbilical Cord Mesenchymal Stem Cells into Prostate-Like Epithelial Cells In Vivo.. Cited on 23 Juli 2014.
  9. Rantam FA, 2014, Stem Cell, Mesenchymal, Hemopoetik dan Model Aplikasi. Surabaya: Airlangga University Press; 33-34.
  10. Rodriguez J, 2015, Intradermal injection of human adipose-derived stem cells accelerates skin wound healing in nude mice. Cited on 16 Nopember 2015.
  11. Romo,T. Oct 10, 2012, Skin Wound Healing. Medscape reference.
  12. SCCR Laboratory, 2015, RSI Sultan Agung Semarang.
  13. Sjamsuhidajat R, Wim De Jong, 2010, Buku Ajar Ilmu Bedah. Jakarta , EGC;: 95-110.
  14. Sukpat S, Isarasena N, Wongphoom J, Patumraj S. 2013. Vasculoprotective Effects of Combined Endothelial Progenitor Cells and Mesenchymal Stem Cells in Diabetic Wound Care:Their Potential Role in Decreasing Wound-Oxidative Stress. Cited on 15 Mei 2013.

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ISSN: 2339-093X (Online) | 2085-1545 (Print)
DOI : 10.30659/sainsmed

This work is licensed under a Creative Commons Attribution 4.0 International License

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