Anxiolytic effect of aqueous extract of moringa oleifera in balb/c mice

Ririn Dwi Pratiwi, Sri Kuswandari, Rinaldi Utomo

Abstract


Background: Anxiety control is required to achieve successful dental treatment especially in children. Dental anxiety is exaggerated fear or worry associated with dental treatment and it is followed with a sense of losing control. Moringa oleifera has been claimed as a potential plant to treat anxiety. A large amount of flavonoid in Moringa oleifera leaves is known to have anxiolytic effect due to their interaction with gamma-aminobutyricacid (GABA) receptors like benzodiazepine molecule. The aim of this study was to determine the anxiolytic effect of aqueous extract of Moringa oleifera (AEMO) leaves in balb/c mice.
Method: Subject of this study was 25 mice divided into 5 groups. Diazepam as a positive control (K+), CMC-Na 0.5% as a negative control (K-), aqueous extract of M. oleifera leaves 375 mg/kgBW (P1), 500 mg/kgBW (P2), and 625 mg/kgBW (P3). Tests were conducted using Elevated Plus Maze. Before and after data were analyzed with paired T-test, differences of anxiety score between groups was analyzed with One-way ANOVA.
Result: Group P2 and P3 significantly reduce anxiety in mice (p <0.05). The anxiolytic effect showed by group P2, P3 and K(+) were significantly different (p <0.05) compared to group K(-).
Conclusion: Aqueous extract of Moringa oleifera leaves produced its anxiolytic effect at 500mg/kgBW and 625mg/kgBW peroral.

Keywords


Anxiety; Anxiolytic; Moringa oleifera

Full Text:

PDF

References


Klingberg G, Broberg AG. Dental fear/anxiety and dental behaviour management problems in children and adolescents: a review of prevalence and concomitant psychological factors. Int J Paediatr Dent. 2007;17(6):391-406.

Imamullah AY, Utomo RB, Supartinah A. Comparison of dental anxiety levels measured using Dental Anxiety Scale and GSR-Psychoanalyzer in patients aged 6-8 years old. ODONTO Dental Journal. 2022;9(1): 1-11.

Drajat RS, Wardhana ES, Rochmah YS. Perbedaan pengaruh musik instrumental kitaro dan musik tradisional langgam jawa terhadap tingkat kecemasan anak – anak sebelum tindakan perawatan gigi. ODONTO Dental Journal. 2017;4(1): 21–6.

Newton T, Asimakopoulou K, Daly B, Scambler S, Scott S. The management of dental anxiety: time for a sense of proportion?. Br Dent J. 2012;213(6):271â€4.

Orret EO, Hertz MB. Anxiety Control in the Dental Patient. Dental Clinic N Am. 2012;(56):1–16.

Sharma K, Kumar N, Raj K, Niazi J, Gupta V. Anti-anxiety activity of Eriobotrya japonica leaf extracts. Res Journal of Pharmacy Biology Chemistry Science. 2011;(2):255-9.

Abalaka ME, Olonitola OS, Onaolapo JA, Inabo HI. Evaluation of acute toxicity of Moimordixa charantnia extract, using wistar rats to determine safety level and usefulness of the plant ethnochemotheraphy. Int. Jor. P. App. Scs. 2009;3(4):1-6.

Khawaja TM, Tahira M, Ikram UH. Moringa oleifera: a natural gift – a review. Journal of Pharmacy Science Res. 2010;(2):775–81.

Karim N, Khan I, Khan H, Ayub B, Abdel-Halim H, Gavande N. Anxiolytic potential of natural flavonoids. SM J Steroids Horm. 20181;(1):1001.

Poernomo H, Setiawan DS. The effect of pegagan gel (centella asiatica (l.) Urban) on wound healing processes in mice (mus musculus) in vivo. ODONTO Dental Journal. 2022;9(1): 137–46.

Jäger AK, Saaby L. Flavonoids and the CNS. Molecules. 2011;(16):1471–85.

Crawley JN. Emotional behaviors: animal models of psychiatric diseases. What's Wrong With My Mouse? Behavioral phenotyping of transgenic and knockout mice. 2nd Ed. New York: John Wiley and Sons. 2007. 226-65.

Komada M., Takao K, Miyakawa T. Elevated plus maze for mice. J Vis Ex. 2008;(22):1088.

Ingale SP, Gandhi FP. Effect of aqueous extract of moringa oleifera leaves on pharmacological models of epilepsy and anxiety in mice. International Journal of Epilepsy. 2016;3(1):12-9

Anief, M. Perjalanan dan Nasib Obat Dalam Badan. UGM Press. Yogyakarta. 1990:5-10.

Awodele O, Oreagba IA., Odoma S, da Silva JA, Osunkalu VO. Toxicological evaluation of the aqueous leaf extract of Moringa oleifera lam. (moringaceae). Journal of Ethnopharmacology. 2012;139(2): 330-336.

Calabrese EJ. An assessment of anxiolytic drug screening tests: hormetic dose responses predominate. Crit Rev Toxicol. 2008;38(6): 489-542

File SE, Lippa AS, Beer B, Lippa MT. Animal tests of anxiety. Curr Protoc Neurosci. 2004;26(1):1-22.

Nuss P. Anxiety disorders and GABA neurotransmission: a disturbance of modulation. Neuropsychiatr Dis Treat. 2015;(11):165–75.

Bhattacharya A, Santra S, Mahapatra S, et al. Study of anxiolytic effect of ethanolic extract of drumstick tree leaves on albino mice in a basic neuropharmacology laboratory of a postgraduate teaching institute. J Health Res Rev. 2016;3(2): 41-7.




DOI: http://dx.doi.org/10.30659/odj.10.1.1-6

Refbacks

  • There are currently no refbacks.


Lisensi Creative Commons
This work is licensed under a Lisensi Creative Commons Atribusi-BerbagiSerupa 4.0 Internasional.
Contact us: Odonto Dental Journal: Jl. Raya Kaligawe Km.4, PO BOX 1054/SM Semarang, Central Java, Indonesia, 50112. Email: odontodentaljournal@unissula.ac.id