Gadjah Mada University - Indonesia
Anxiolytic effect of aqueous extract of moringa oleifera in balb/c mice
Ririn Dwi Pratiwi
Sri Kuswandari
Gadjah Mada University
Departement of Pediatric Dentistry, Faculty of Dentistry, Universitas Gadjah Mada
Rinaldi Utomo
Gadjah Mada University
Departement of Pediatric Dentistry, Faculty of Dentistry, Universitas Gadjah Mada
(*) Corresponding Author
Background: Anxiety control is required to achieve successful dental treatment especially in children. Dental anxiety is exaggerated fear or worry associated with dental treatment and it is followed with a sense of losing control. Moringa oleifera has been claimed as a potential plant to treat anxiety. A large amount of flavonoid in Moringa oleifera leaves is known to have anxiolytic effect due to their interaction with gamma-aminobutyricacid (GABA) receptors like benzodiazepine molecule. The aim of this study was to determine the anxiolytic effect of aqueous extract of Moringa oleifera (AEMO) leaves in balb/c mice.
Method: Subject of this study was 25 mice divided into 5 groups. Diazepam as a positive control (K+), CMC-Na 0.5% as a negative control (K-), aqueous extract of M. oleifera leaves 375 mg/kgBW (P1), 500 mg/kgBW (P2), and 625 mg/kgBW (P3). Tests were conducted using Elevated Plus Maze. Before and after data were analyzed with paired T-test, differences of anxiety score between groups was analyzed with One-way ANOVA.
Result: Group P2 and P3 significantly reduce anxiety in mice (p <0.05). The anxiolytic effect showed by group P2, P3 and K(+) were significantly different (p <0.05) compared to group K(-).
Conclusion: Aqueous extract of Moringa oleifera leaves produced its anxiolytic effect at 500mg/kgBW and 625mg/kgBW peroral.
Method: Subject of this study was 25 mice divided into 5 groups. Diazepam as a positive control (K+), CMC-Na 0.5% as a negative control (K-), aqueous extract of M. oleifera leaves 375 mg/kgBW (P1), 500 mg/kgBW (P2), and 625 mg/kgBW (P3). Tests were conducted using Elevated Plus Maze. Before and after data were analyzed with paired T-test, differences of anxiety score between groups was analyzed with One-way ANOVA.
Result: Group P2 and P3 significantly reduce anxiety in mice (p <0.05). The anxiolytic effect showed by group P2, P3 and K(+) were significantly different (p <0.05) compared to group K(-).
Conclusion: Aqueous extract of Moringa oleifera leaves produced its anxiolytic effect at 500mg/kgBW and 625mg/kgBW peroral.
Keywords: Anxiety; Anxiolytic; Moringa oleifera
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