EFFECT OF FIBROIN SPONGE ON ALVEOLAR BONE REMODELING PROCESS POST TOOTH EXTRACTION

Sartika Puspita, Lutfia Siti Hanifatunnisa, Agustin Wulan Suci Dharmayanti, Erlina Sih Mahanani, Edwyn Saleh

Abstract


Background: There are still quite a lot of cases of tooth extraction in Indonesia because of the cavities are not treated or because of other cases. Extraction is the main cause of 90% of bone destruction and a risk of infection and bleeding. Fibroin sponge is a natural polymer material derived from Bombyx mori silk cocoon which contains of 18 amino acids and have a good mechanically, biodegradable and biocompatible. This study aimed to determine the effect of fibroin sponge on the remodeling of post tooth extraction sockets.
Method: This study was a laboratory experiment with quasy experimental design using 30 Sprague dawley rats divided into 2 groups. Each group had the mandibular left incisor extracted. The first group as a control was given 15 ug of Curaspon and the second group was given 15 ug of fibroin sponge intra alveolar.
Result: The results were observed after the 3rd, 7th, 14th, 21st, 28th days under a light microscope and identified by scoring bone remodeling based on predetermined criteria. Data were analyzed using the Mann-Whitney Test followed by the Kruskall-Wallis Test. The results showed that there was no difference in the process of bone remodeling post tooth extraction in the fibroin sponge group compared to Curaspon (p>0.05).
Conclusion: Fibroin sponge has the same effect on the bone remodeling process as Curaspon.


Keywords


Alveolar bone; Bone remodeling; Fibroin sponge; Post tooth extraction

References


Jannah Tamara, A.H., Rochmah, Y.S., Mujayanto, R., 2015. Pengaruh Aplikasi Virgin Coconut Oil Terhadap Peningkatan Jumlah Fibroblas Pada Luka Pasca Pencabutan Gigi Pada Rattus Novergicus. Odonto Dent. J. 1, 29.

Hermanto, E., Sari, R.P., Imaniar, A.C.D., Anggoro, K., 2018. Grafting effectiveness of Anadara granosa shell combined with sardinella longiseps gel on the number of osteoblast-osteoclast cells. Dent. J. Maj. Kedokt. Gigi 50, 138.

Le, B.T., 2008. Management of Complications of Dental Extractions 8.

Sularsih, Soeprijanto, 2012. Perbandingan Jumlah Sel Osteoblas Pada Penyembuhan Luka Antara Penggunaan Kitosan Gel 1% dan 2 %. J. Mater. Kedokt. Gigi 145–152.

ElShiha, H.Y., Marzouk, H.A.E.-M., 2012. Efficacy Of Chitosan And Absorbable Gelatin Sponge On Hemostasis And Wound Healing Following Tooth Extraction “A Comparative Study†58, 6.

Mani, A., Anarthe, R., Preeti, K., Maniyar, S., Anuraga, S., 2018. Hemostatic Agents in Dentistry. Galore Int. J. Health Sci. Res. 3.

Ju, H.W., Lee, O.J., Lee, J.M., Moon, B.M., Park, H.J., Park, Y.R., Lee, M.C., Kim, S.H., Chao, J.R., Ki, C.S., Park, C.H., 2016. Wound healing effect of electrospun silk fibroin nanomatrix in burn-model. Int. J. Biol. Macromol. 85, 29–39.

Puspita, S., Soesatyo, M.H., Sunarintyas, S., Mulyawati, E., 2019. The fibroin (Bombyx mori L.) is cytocompatible with human primary pulp cells. Presented at the 1st International Conference On Bioinformatics, Biotechnology, And Biomedical Engineering (BIOMIC 2018), Yogyakarta, Indonesia, p. 020018.

Rockwood, D.N., Preda, R.C., Yücel, T., Wang, X., Lovett, M.L., Kaplan, D.L., 2011. Materials fabrication from Bombyx mori silk fibroin. Nat. Protoc. 6, 1612–1631.

Suzuki, M., Taira, Y., Kato, C., Shinkai, K., Katoh, Y., 2016. Histological evaluation of direct pulp capping of rat pulp with experimentally developed low-viscosity adhesives containing reparative dentin-promoting agents. J. Dent. 44, 27–36.

Budi, H. S., Soesilowati, P. dan Imanina, Z. (2017) ‘Gambaran Histopatologi Penyembuhkan Luka Pencabutan Gigi pada Makrofag dan Neovaskular dengan Pemberian Getah Batang Pisang Ambon’, Majalah Kedokteran Gigi Indonesia, 3(3), 3.

Endrawati, Y.C., Solihin, D.D., Suryani, A., Subyakto, S. (2017) ‘Optimasi Rendemen Fibroin Ulat Sutera Bombyx mori L. dan Attacus atlas L. dengan Response Surface Methodology’, Agritech, 37(2), 205.

Rostiny, R., Djulaeha, E., Hendrijantini, N., Pudijanto, A. (2016) ‘The Effect Of Combined Moringa Oleifera and Demineralized Freeze-Dried Bovine Bone Xenograft On The Amount Of Osteoblast And Osteoclast In The Healing Of Tooth Extraction Socket Of Cavia cobaya’, Dental Journal (Majalah Kedokteran Gigi), 49(1), 37.

Primadina, N., Basori, A. dan Perdanakusuma, D. S. (2019) ‘Proses Penyembuhan Luka Ditinjau dari Aspek Mekanisme Seluler dan Molekuler’, Qanun Medika - Medical Journal Faculty of Medicine Muhammadiyah Surabaya, 3(1), 31.

Farokhi, M., Mottaghitalab, F., Fatahi, Y., Khademhosseini, A., Kaplan, D.L. (2018) ‘Overview of Silk Fibroin Use in Wound Dressings’, Elsevier, 36.

Rustam, A., Tatengkeng, F., Fahruddin, A.M., Djais, A.I. (2017) ‘Kombinasi Perancah Silk-Fibroin Dari Kepompong Ulat Sutera (Bombyx mori) dan Konsentrat Platelet Sebagai Inovasi Terapi Regenerasi Tulang Alveolar’; 9.Rutkovskiy, A., Stensløkken, K.-O. and Vaage, I. J. (2016) ‘Osteoblast Differentiation at a Glance’, Medical Science Monitor Basic Research, 22, 95–106.

Zafar, M. S. dan Al-Samadani, K. H. (2014) ‘Potential Use Of Natural Silk For Bio-Dental Applications’, Journal of Taibah University Medical Sciences, 9(3), 171–177.

Huttunen, M.M., Pekkinen, M., Ahlström, M.E.B., Lamberg-Allardt, C.J.E. (2007) ‘Effects Of Bioactive Peptides Isoleucine-Proline-Proline (IPP), Valine-Proline-Proline (VPP) and Leucine-Lysine-Proline (LKP) On Gene Expression Of Osteoblasts Differentiated From Human Mesenchymal Stem Cells’, British Journal of Nutrition, 98(04).

Purwaningsih, S., Salamah, E. dan Apriyana, G. P. (2013) ‘Profil Protein dan Asam Amino Keong Ipong-Ipong (Fasciolaria salmo) pada Pengolahan yang Berbeda’, Jurnal Gizi dan Pangan, 8(1), 77.

Handayani, B. and Brahmanta, A. (2012) ‘Jumlah Osteoblas pada Daerah Tarikan dengan Pemberian Ekstrak Propolis Sebagai Pencegahan Relaps Ortodonti’, Denta Jurnal Kedokteran Gigi, 12.

Melke, J., Midha, S., Ghosh, S., Ito, K., Hofmann, S. (2015) ‘Silk Fibroin As Biomaterial For Bone Tissue Engineering’, Elsevier.

Westby, M.J., Dumville, J.C., Stubbs, N., Norman, G., Wong, J.K., Cullum, N., Riley, R.D. (2018) ‘Protease Activity As A Prognostic Factor For Wound Healing In Venous Leg Ulcers’, Cochrane Database of Systematic Reviews. Edited by Cochrane Wounds Group. Molekuler’, Qanun Medika - Medical Journal




DOI: http://dx.doi.org/10.30659/odj.9.0.7-15

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