The Effect of Administration of Longan (DimocarpusLonganL Steud)Leaf Extract Cream on Collagen Density and MMP-1 Expression in the Dermis of BALB/C Mice Exposed to UV-B Rays

Ruri Eka Maryam Mulyaningsih*  -  Medical Faculty, Universitas Islam Sultan Agung/ Medical Faculty Unswagati Cirebon, Indonesia
Atina Hussaana  -  Medical Faculty, Universitas Islam Sultan Agung, Indonesia
Chodidjah Chodidjah  -  Medical Faculty, Universitas Islam Sultan Agung, Indonesia

(*) Corresponding Author
DOI: 10.30659/sainsmed.v10i2.3341

INTRODUCTION: UV-B exposure to the skin may produce ROS, decreasing collagen density and increasing MMP-1 expression. Longan leaf extract cream contains antioxidant and is expected to prevent decrease in collagen density and MMP-1 expression increase.

OBJECTIVE: The research aims at administering longan leaf extract cream to increase collagen density and decrease MMP-1 expression in BALB/c mice exposed to UV-B rays.

METHODS: This experimental research employed a Post Test Only Controlled Group Design. The subjects were 24 BALB/c mice, which were randomly divided into 4 groups. The Control Group (Ctr-G)was only administered with basic cream and exposed to UV-B rays, while Groups DLS-3, DLS-6, and DLS-13were respectively administered with longan leaf extract cream at doses 3.25%, 6.5% and 13% and exposed to UV-B rays for 4 weeks at a dose of 1 MED. On day 29, a tissue excision biopsy was performed to determine the collagen density and MMP-1 expression. The collagen density preparations were made using Masson’s Trichome staining and MMP-1 preparations were made using immunohistochemical staining.

RESULTS: Kruskalwallis analysis showedstatistically no significant differences in collagen density and MMP-1 expression between groups(p value> 0.05).

CONCLUSION: Administration of longan leaf extract cream at doses of 3.25%, 6.5% and 13% had proven unable to increase collagen densitynor decrease the MMP-1 expression significantly after UVB exposure.

Keywords: longan leaf extract, collagen density, MMP-1 expression

  1. Ackermann, L. et al. (2017) ‘An epidemic of allergic contact dermatitis caused by a new allergen, caprylhydroxamic acid, in moisturizers’, Contact Dermatitis. doi: 10.1111/cod.12787.
  2. Afaq, F. and Katiyar, S. K. (2011) ‘Polyphenols: skin photoprotection and inhibition of photocarcinogenesis.’, Mini Reviews in Medicinal Chemistry, 11(14), pp. 1200–15. doi: 10.1007/s00403-009-1001-3.Skin.
  3. Apriyanto, D. R. (2014) ‘Efek dan mekanisme antiviral ekstrak metanol daun Dimocarpus longan Lour . terhadap virus hepatitis C’, p. 23.
  4. Apriyanto, D. R. et al. (2016) ‘Anti-hepatitis c virus activity of a crude extract from longan (Dimocarpus longan Lour.) leaves’, Japanese Journal of Infectious Diseases, 69(3), pp. 213–220. doi: 10.7883/yoken.JJID.2015.107.
  5. Bae, J. Y. et al. (2010) ‘Dietary compound ellagic acid alleviates skin wrinkle and inflammation induced by UV-B irradiation’, Experimental Dermatology, 19(8). doi: 10.1111/j.1600-0625.2009.01044.x.
  6. Bosch, R. et al. (2015) ‘Mechanisms of Photoaging and Cutaneous Photocarcinogenesis, and Photoprotective Strategies with Phytochemicals’, Antioxidants. doi: 10.3390/antiox4020248.
  7. Dahlan, M. S. (2014) Statistik untuk Kedokteran dan Kesehatan. 6th edn, epidemiologi Indonesia. 6th edn. Edited by M. S. Dahlan. Jakarta: Epidemiologi Indonesia. doi: 10.1017/CBO9781107415324.004.
  8. Darmojo, R. B. (2011) Buku Ajar Boedhi-Darmojo GERIATRI (Ilmu Kesehatan Usia Lanjut). Edisi IV. Edited by Hadi Martono; Kris Pranarka. Jakarta: Balai Penerbit Fakultas Kedokteran Universitas Indonesia.
  9. DR.Dr M Yulianto Listiawan,Sp.KK(K), F. (2016) etiologi fotoaging. Available at: www.beritasatu.com/.../349210-80-penuaan-dini-disebabkan-radiasi-ultraviolet.html (Accessed: 2 January 2018).
  10. Farage, M. A., Miller, K. W. and Maibach, H. I. (2010) Textbook of aging skin, Textbook of Aging Skin. doi: 10.1007/978-3-540-89656-2.
  11. Fouche, G. (2013) ‘Anticancer Activity of Dimocarpus longan Lour . Leaf Extracts in vitro and Phytochemical Profile By Extracts in vitro and Phytochemical Profile’, Greener Journal of Medicinal Plant Research, 1, pp. 001–005.
  12. Fratzl, P. (2008) Collagen: Structure and mechanics, Collagen: Structure and Mechanics. doi: 10.1007/978-0-387-73906-9.
  13. Gilchrest, B. A. (2013) ‘Photoaging.’, The Journal of investigative dermatology, 133(E1). doi: 10.1038/jid.2013.2.
  14. Green, A. C. et al. (2011) ‘Factors associated with premature skin aging (photoaging) before the age of 55: A population-based study’, Dermatology, 222(1), pp. 74–80. doi: 10.1159/000322623.
  15. Han, A., Chien, A. L. and Kang, S. (2014) ‘Photoaging’, Dermatologic Clinics, pp. 291–299. doi: 10.1016/j.det.2014.03.015.
  16. Handayani, F. W. et al. (2003) ‘REVIEW: AKTIVITAS ANTI INFLAMASI DAN BIOAVAIBILITAS ANDROGRAFOLID PADA HEWAN UJI’, Farmaka.
  17. Helfrich, Y. R., Sachs, D. L. and Voorhees, J. J. (2008) ‘Overview of skin aging and photoaging.’, Dermatology nursing / Dermatology Nurses’ Association, 20(3), pp. 177–83. Available at: http://www.ncbi.nlm.nih.gov/pubmed/18649702.
  18. Hwang, E. et al. (2013) ‘A comparative study of baby immature and adult shoots of Aloe vera on UVB-induced skin photoaging in vitro’, Phytotherapy Research. doi: 10.1002/ptr.4943.
  19. Jackson-Richards, D. (2014) ‘Dermatology for Skin of Color’, in Dermatology Atlas for Skin of Color, pp. 67–72. doi: 10.1007/978-3-642-54446-0_13.
  20. Jung, H. Y. et al. (2014) ‘Pinus densiflora extract protects human skin fibroblasts against UVB-induced photoaging by inhibiting the expression of MMPs and increasing type I procollagen expression’, Toxicology Reports. doi: 10.1016/j.toxrep.2014.08.010.
  21. Kim, S.-Y. et al. (2004) ‘Protective effects of dietary soy isoflavones against UV-induced skin-aging in hairless mouse model.’, Journal of the American College of Nutrition, 23(April 2014), pp. 157–162. doi: 10.1080/07315724.2004.10719356.
  22. Kurniasari, D. (2010) ‘Pengaruh Enhancer Propilenglikol terhadap Karakteristik sediaan dan penetrasi Natrium Diklofenak Dalam Basis Krim w/o (Penetrasi menggunakan Membran Kulit Tikus Wistar)’. Available at: http:/lib.unair.ac.id.
  23. Liebel, F. et al. (2012) ‘Irradiation of skin with visible light induces reactive oxygen species and matrix-degrading enzymes’, Journal of Investigative Dermatology. doi: 10.1038/jid.2011.476.
  24. Mortimer, P. S. and Rockson, S. G. (2014) ‘New developments in clinical aspects of lymphatic disease’, Journal of Clinical Investigation. doi: 10.1172/JCI71608.
  25. Muthukumarasamy, R. et al. (2016) ‘Formulation and evaluation of natural antioxidant cream comprising methanolic peel extract of Dimocarpus longan’, International Journal of Pharmaceutical and Clinical Research, 8(9), pp. 1305–1309.
  26. Nichols, J. A. and Katiyar, S. K. (2010) ‘Skin photoprotection by natural polyphenols: Anti-inflammatory, antioxidant and DNA repair mechanisms’, Archives of Dermatological Research, pp. 71–83. doi: 10.1007/s00403-009-1001-3.
  27. Pandel, R. et al. (2013) ‘Skin Photoaging and the Role of Antioxidants in Its Prevention’, ISRN Dermatology, 2013, pp. 1–11. doi: 10.1155/2013/930164.
  28. Panyathep, A. et al. (2013) ‘Antioxidant and anti-matrix metalloproteinases activities of dried longan (Euphoria longana) seed extract’, ScienceAsia, 39(1), pp. 12–18. doi: 10.2306/scienceasia1513-1874.2013.39.012.
  29. Park, J. E. et al. (2014) ‘The protective effect of kaempferia parviflora extract on UVB-induced skin photoaging in hairless mice’, Photodermatology Photoimmunology and Photomedicine. doi: 10.1111/phpp.12097.
  30. Pittayapruek, P. et al. (2016) ‘Role of matrix metalloproteinases in Photoaging and photocarcinogenesis’, International Journal of Molecular Sciences. doi: 10.3390/ijms17060868.
  31. Qing Wu, Li Wang, Xiaolin Yu, Y. S. and Y. J. (2013) ‘Polyphenols from Longan Leaf and Their Radical-Scavenging Activity’, 2013 4th International Conference on Food Engineering and Biotechnology IPCBEE vol.50 (2013) © (2013) IACSIT Press, Singapore DOI: 10.7763/IPCBEE. 2013. V50. 36, 50(36), pp. 180–185. doi: 10.7763/IPCBEE.
  32. Quan, T. et al. (2009) ‘Matrix-degrading metalloproteinases in photoaging’, in Journal of Investigative Dermatology Symposium Proceedings, pp. 20–24. doi: 10.1038/jidsymp.2009.8.
  33. R.K., S. et al. (2013) ‘Review on skin aging and compilation of scientific validated medicinal plants, prominence to flourish a better research reconnoiters in herbal cosmetic’, Research Journal of Medicinal Plant, pp. 1–22. doi: 10.3923/rjmp.2013.1.22.
  34. Nanti Hermayani (1998) ‘Pengaruh Basis Hidrokarbon, Basis Krim O/W non ionik dan Basisi Serap terhadap Bioavaibilitas Asam salisilat secara Perkutan pada Kelinci’. Available at: http://digilib.ubaya.ac.id/pustaka.php/151174.
  35. Sahu, R. K. et al. (2013) ‘Review on Skin Aging and Compilation of Scientific Validated Medicinal Plants, Prominence to Flourish a Better Research Reconnoiters in Herbal Cosmetic’, Research Journal of Medicinal Plant. doi: 10.3923/rjmp.2013.1.22.
  36. Salamah, N. and Widyasari, E. (2015) ‘Aktivitas Antioksidan Ekstrak Metanol Daun Kelengkeng (Euphoria longan ( L ) Steud.) Dengan Metode Penangkapan Radikal 2,2’ Difenil-1-Pikrilhidrazil’, Pharmaciana. doi: 10.12928/pharmaciana.v5i1.2283.
  37. Shoulders, M. D. and Raines, R. T. (2010) ‘Collagen Structure and Stability’, Annual Review of Biochemistry. doi: 10.1146/annurev.biochem.77.032207.120833.COLLAGEN.
  38. Tilbrook, K. et al. (2013) ‘The UVR8 UV-B Photoreceptor: Perception, Signaling and Response’, The Arabidopsis Book. doi: 10.1199/tab.0164.
  39. Tobin, D. J. (2017) ‘Introduction to skin aging’, Journal of Tissue Viability, 26(1), pp. 37–46. doi: 10.1016/j.jtv.2016.03.002.
  40. Vicentini, F. T. M. C. et al. (2011) ‘Quercetin inhibits UV irradiation-induced inflammatory cytokine production in primary human keratinocytes by suppressing NF-κB pathway’, Journal of Dermatological Science. doi: 10.1016/j.jdermsci.2011.01.002.
  41. vita .Tawaran (2016) ‘Efek Penggunaan Probiotik Topikal Terhadap Ekspresi MMP-13 dan Kolagen III pada Lapisan Dermis Tikus yang Dipapar Sinar Ultraviolet’, Jurnal Farmasi Klinik Indonesia, 5(2), pp. 138–148. doi: 10.15416/ijpc.2016.5.2.138.
  42. Watson, R. E. B. et al. (2014) ‘Damage to Skin Extracellular Matrix Induced by UV Exposure’, Antioxidants & Redox Signaling. doi: 10.1089/ars.2013.5653.
  43. Xue, Y. et al. (2015) ‘Two new flavonol glycosides from Dimocarpus longan leaves’, Natural Product Research, 29(2), pp. 163–168. doi: 10.1080/14786419.2014.971318.
  44. Yin, Y. et al. (2013) ‘Quercitrin protects skin from UVB-induced oxidative damage’, Toxicology and Applied Pharmacology. doi: 10.1016/j.taap.2013.03.015.
  45. Yuge Liu (2012) ‘Antioxidant activity of longan (Dimocarpus longan) barks and leaves’, African Journal of Biotechnology, 11(27), pp. 7038–7045. doi: 10.5897/AJB11.3297.

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