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The Effect of Administration of Longan (DimocarpusLonganL Steud)Leaf Extract Cream on Collagen Density and MMP-1 Expression in the Dermis of BALB/C Mice Exposed to UV-B Rays | <hr /> (mysql): SELECT * FROM authors WHERE submission_id = 3341 ORDER BY seq   <hr /> <hr /> (mysql): SELECT * FROM author_settings WHERE author_id = '5516'   <hr /> <hr /> (mysql): SELECT * FROM author_settings WHERE author_id = '13830'   <hr /> <hr /> (mysql): SELECT * FROM author_settings WHERE author_id = '13831'   <hr /> Maryam Mulyaningsih | Sains Medika: Jurnal Kedokteran dan Kesehatan
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The Effect of Administration of Longan (DimocarpusLonganL Steud)Leaf Extract Cream on Collagen Density and MMP-1 Expression in the Dermis of BALB/C Mice Exposed to UV-B Rays

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Ruri Eka Maryam Mulyaningsih*  -  Medical Faculty, Universitas Islam Sultan Agung/ Medical Faculty Unswagati Cirebon, Indonesia
Atina Hussaana  -  Medical Faculty, Universitas Islam Sultan Agung, Indonesia
Chodidjah Chodidjah  -  Medical Faculty, Universitas Islam Sultan Agung, Indonesia

(*) Corresponding Author
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DOI: 10.30659/sainsmed.v10i2.3341'>
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10.30659/sainsmed.v10i2.3341

INTRODUCTION: UV-B exposure to the skin may produce ROS, decreasing collagen density and increasing MMP-1 expression. Longan leaf extract cream contains antioxidant and is expected to prevent decrease in collagen density and MMP-1 expression increase.

OBJECTIVE: The research aims at administering longan leaf extract cream to increase collagen density and decrease MMP-1 expression in BALB/c mice exposed to UV-B rays.

METHODS: This experimental research employed a Post Test Only Controlled Group Design. The subjects were 24 BALB/c mice, which were randomly divided into 4 groups. The Control Group (Ctr-G)was only administered with basic cream and exposed to UV-B rays, while Groups DLS-3, DLS-6, and DLS-13were respectively administered with longan leaf extract cream at doses 3.25%, 6.5% and 13% and exposed to UV-B rays for 4 weeks at a dose of 1 MED. On day 29, a tissue excision biopsy was performed to determine the collagen density and MMP-1 expression. The collagen density preparations were made using Masson’s Trichome staining and MMP-1 preparations were made using immunohistochemical staining.

RESULTS: Kruskalwallis analysis showedstatistically no significant differences in collagen density and MMP-1 expression between groups(p value> 0.05).

CONCLUSION: Administration of longan leaf extract cream at doses of 3.25%, 6.5% and 13% had proven unable to increase collagen densitynor decrease the MMP-1 expression significantly after UVB exposure.

Keywords: longan leaf extract, collagen density, MMP-1 expression

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DOI : 10.30659/sainsmed

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