Ame Suciati Setiawan, Fajar Fatriadi, Diani Prisinda


Background: Ocimum americanum (O. americanum) is a natural plant with antibacterial effects inhibits the growth of gram-positive and gram-negative anaerobic bacteria in the oral cavity. Enterococcus faecalis (E. faecalis) is a gram-positive anaerobic bacteria in the root canal which is resistance to intracanal medicament. The purpose of this study is to analyze the antibacterial effect of ethanol fractionation of O. americanum towards E. faecalis ATCC 29212. Ocimum americanum is a natural plant that has antibacterial properties against grampositive and gram-negative anaerobic bacteria in the oral cavity. Enterococcus faecalis is an anaerobic gram-negative bacteria was found in root canals and resistant to various root canal medicament. The purpose of this study was to analyze the antibacterial effect of the ethanol fraction of Ocimum americanum against Enterococcus faecalis.
Methods: The research method was experimental used disc diffusion technique. The fraction is made from solid extract into a polar solution with ethanol solvent with concentration 20%, 40%, 60% and 80%. The test was performed by measuring the inhibition zones around the disc after 24 hours.
Results: Chemical properties of O.americanum are alkaloid, flavonoid, saponin, tanin, steroid, dan terpenoid. Inhibition zone of O. americanum fraction with concentration 20%, 40%, 60% and 80% is 8.8 cm, 9.8 cm, 11.1 cm and 16.7 cm. The measurement of O. americanum inhibition zone showed better results compared than positive control (Minosep).
Conclusion: Ethanol fraction of O.americanum has antibacterial effect towards E. faecalis.


Enterococcus faecalis ; Fraksi Etanol ; Ocimum Americanum


Sroisiri.T., Boonyanit.T. Phytopharmacy Journal Ocimum americanum Ocimum americanum L. Essential Oil Exhibits Antimicrobial Activity Against Oral Bacteria Related To Periodontal Disease. Phytopharm.2012

Sroisiri.T., Boonyanit.T. In Vitro Antimicrobial Activity Of Ocimum americanum L. Essential Oil Against Oral Microorganisms. South Asian J Trop Med Public Health, 2009;40:1025-1033.

Rocas, I.N & Siqueira, J.F. Characterization Of Microbiota Of Root Canal Treated Teeth With Post Treatment Disease. Journal Of Clinical Microbiology, 2012;50(5): 1721-1724

Torabinejad M, Walton RE. Endodontics: Principles and Practice, 5th edition. Elsevier. 2014

Hargreaves KM, Berman LH. Cohen’s:Pathways of The Pulp Eleventh Edition.Elsevier. 2016

Dammanschke. T., Jung. N., Harks. I., Schafer.E. The Effect Of Different Root Canal Medicament Of Enterococcus Faecalis Ex Vivo. European Journal Of Dentistry, 2013;7(4): 442-448

Abbaszadegan. A., Sahebi. S., Gholami. A., Delroba. A., Kiani. A., Iraji. A. Time Dependent Antibacetrial Efects Of Aloe Vera And Zataria Multiflora Plant Essential Oils Compared To Calcium Hydroxide In Teeth Infected With Enterococcus Faecalis. Journal Of Investigative And Clinical Dentistry, 2016;7(1): 93-101

M.A. Mozayeni, A. Haeri, O. Dianat, A.R. Jafari. Antimicrobial Effect Of Four Intracanal Medicaments On Enterococcus Faecalis; An In Vitro Study. Iranian Endodontic Journal, 2014;9(3):195-198

Prisinda.D., Suciati.A., Fatriadi.F. Antibacterial Potential Of Ocimum Sanctum Oils In Relation To Enterococcus Faecalis Atcc 29212. Dental Journal, 2018;51(3): 104–107

A.S. Setiawan., Fatriadi. F., Prisinda.D. Differences Of Basilicum Leaf (Ocimum Basilicum) Essential Oil’s Inhibition Zones And Parachlorophenol (Chkm) Against Enterococcus Faecalis. International Journal Of Medical Science And Clinical Invention, 2018; 5(12): 4237-4239

D. Kandaswamy., Venkateshbabu N. Root canal irrigants. Journal of Conservative Dentistry 2010. 13 (4); 256-264

Siti S., Diki P.W., Sohadi W. Uji Anti Bakteri Obat Kumur Ekstrak Etanol Herba Kemangi (Ocimum americanum L) Terhadap Streptococcus mutans. Indonesian Journal of Pharmaceutical Science and Technology. 2012.1 (2):20-8.

Diah Ayu L,. Apriliana E. Efek Potensial Daun Kemangi (Ocimum basilicum L) Sebagai Pemanfaatan Hand Sanitizer. Majority. 2016. 5(5):124-128.

Livia Sobodnikova., Silvia Fialova et all. Antibiofilm Activity of Plant Polyphenols. Molecules. 2016. 21(1717):1-15.

Garg N, Garg A. Textbook of Endodontics 2nd edition. 3rd ed. India: Jaypee Brothers Medical Publishers; 201. 555

Horner C, Mawer D, Wilcox M. Reduced susceptibility to chlorhexidine in staphylococci: Is it increasing and does it matter? J Antimicrob Chemother. 2012;67(11):2547–59.

DOI: http://dx.doi.org/10.30659/odj.7.2.111-116


  • There are currently no refbacks.

Lisensi Creative Commons
Ciptaan disebarluaskan di bawah Lisensi Creative Commons Atribusi-BerbagiSerupa 4.0 Internasional.
Contact us: ODONTO Dental Journal: Jl. Raya Kaligawe Km.4, PO BOX 1054/SM Semarang 50112. Email: [email protected]