Universitas Muhammadiyah Surakarta - Indonesia
Conservative Dentistry
Antibacterial Effectiveness Of Green Shell Nanocitosan (Perna Viridis) On The Growth Of The Bacteria Prevotella Intermedia (In Vitro Study)
Noor Hafida Widyastuti
Cahyani Cahyani
Universitas Muhammadiyah Surakarta - Indonesia
CONSERVATIVE DEPARTMENT
(*) Corresponding Author
Background: Green mussel shell nanochitosan is a size modification of chitosan obtained from green mussel shells that have been processed with specific standards. Nanochitosan from green mussel shells has not been researched for its anti-bacterial properties against Prevotella intermedia bacteria
Purpose: This study aims to determine the antibacterial effect on the growth of Prevotella intermedia bacteria. Method: This research is laboratory research using a posttest only control group design.
Method: This research is laboratory research using a posttest only control group design
Result: Nanochitosan with concentrations of 0.5%, 1%, 1.5% and 2% was able to inhibit the growth of Prevotella intermedia bacteria. The test results using the One Way ANOVA test obtained a value of p = 0.000 so that p < 0.5 and it was stated that there was a significant difference between treatment groups on the growth inhibition value of Prevotella intermedia bacteria
Conclusion: There is an anti-bacterial effect and there is an inhibitory zone in the culture of Prevotella intermedia bacteria that has been incubated within 24 hours with 37°C. The greater the concentration used, the more effective the antibacterial agent is
Keywords: anti-bacterial test, green mussel shell, in vitro test, nano-chitosan, Prevotella intermedia
- Bassam S, El-Ahmar R, Salloum S, Ayoub S. Endodontic postoperative flare-up: An update. Saudi Dent J [Internet]. 2021;33(7):386–94. Available from: https://doi.org/10.1016/j.sdentj.202 1.05.005
- Widyastuti NH, Nurhabibah G. Nonvital Root Canal Treatment ofNecrotic Maxillary Left Lateral Incisor : A Case Report. Int Summit Sci Technol Humanit. 2021;19–24.
- Hemamalini R, Kavitha M, Vishalini A, Arunprasad M. Calcium Hydroxide as an Intracanal Medication-A Review. IOSR J Dent Med Sci e-ISSN[Internet]. 2020;19(10):9–13. Available from: www.iosrjournals.org
- Nadia LMH, Suptijah P, Ibrahim B. Production and Characterization of Chitosan Nano from Black Tiger Shrimp with Ionic Gelation Methods. J Indonesian Fishery Processors. 2014;17(2):119–26.
- Chandrasekaran M, Kim KD, Chun SC. Antibacterial activity of chitosan nanoparticles: A review. Processes. 2020;8(9):1–21.
- Aliasghari A, Khorasgani MR, Vaezifar S, Rahimi F, Younesi H, Khoroushi M. Evaluation of antibacterial efficiency of chitosan and chitosan nanoparticles on cariogenic streptococci: An in vitro study. Iran J Microbiol. 2016;8(2):93–100.
- Divya K, Vijayan S, George TK, Jisha MS. Antimicrobial properties of chitosan nanoparticles: Mode of action and factors affecting activity. Polym Fibers. 2017;18(2):221–30.
- Sanap P, Hegde V, Ghunawat D, Patil M, Nagaonkar N, Jagtap V. Current applications of chitosan nanoparticles in dentistry: A review. Int J Appl Dent Sci. 2020;6(4):81–4.
- Rasaee I, Ghannadnia M, Honari H. Antibacterial properties of biologically formed chitosan nanoparticles using aqueous leaf extract of Ocimum basilicum. Nanomed J. 2016;3(4):240–7.
- Marques B, Cunha N, G. Martins R, Couto J, Santos J, Martins T, et al. MON-593 Thyrotropin Receptor Stimulating Immunoglobulin: Clinical Utility in Graves' Disease Diagnosis. J Endocr Soc [Internet]. 2019 Apr15;3(Supplement_1):MON-593. Available from: https://doi.org/10.1210/js.2019- MON-593
- Mohammadi Z, Shalavi S, Yazdizadeh M. Antimicrobial Activity of Calcium Hydroxide in Endodontics: A Review. Chonnam Med J. 2012;48(3):133.
- Ayu M, Suratri L, Jovina TA. Influence (pH) of saliva on the occurrence of dental caries in preschool children. Research and Development Center for Health Resources and Services. 2017;241–8.
- Priyaa BM, Anitha V, Shanmugam M, Ashwath B, Sylva SD, Vigneshwari S.K. Efficacy of chlorhexidine and green tea mouthwashes in the management of dental plaque-induced gingivitis: A comparative clinical study. Contemp Clin Dent 2015;4(6):505-509.18.
- Raju Ramyathilagam, Divya Angeline, Rajendran Ganesh, John James R. Analogous assay between green tea mouthwash, listerine mouthwash and chlorhexidine mouthwash in plaque reduction, on orthodontic patients: a randomized cross-over study. Int J Community Med Public Health 2017;4(5):1429-1435.19.
- Anggayanti NA, Adiatmika IPG, Adiputra N. Berkumur dengan teh hitam lebih efektif daripada Chlorhexidine gluconate 0,2% untuk menurunkan akumulasi plak gigi. Jurnal PDGI 2013;2(62):35-40.20.
- Sarin S, Marya C, Nagpal R, Oberai SS, Rekhi A. Preliminary clinical evidence of the antiplaque, antigingivitis efficacy of a mouthwash containing 2% green tea-A randomised clinical trial. Oral Health Prev Dent 2015;3(13):197-203.21.
- Tamura M, Ochiai K. Exploring the possible applications of catechin (gel) for oral care of the elderly and disabled individuals. Japanese Dental Science Review 2012;48(2):126-134.
- Masyitah D, Mashudi M, Tumanggor B, Fadriyanti Y. The effect of osteoarthritis exercise on pain and joint range of motion in osteoarthritis patients. Retos. 2024;52:432-7.18.
- Frost K, Naylor AJ, McGettrick HM. The Ying and Yang of Sphingosine-1-Phosphate Signalling within the Bone. Int J Mol Sci. 2023;24(8).19.
- Yang X, Chen L, Xu X, Li C, Huang C, Deng CX. TGF-beta/Smad3 signals repress chondrocyte hypertrophic differentiation and are required for maintaining articular cartilage. J Cell Biol. 2001;153(1):35-46.
