PENGARUH EKSTRAK DAUN BELUNTAS (Pluchea indica (L.) Less) 25% TERHADAP BIOFILM STREPTOCOCCUS MUTANS - in vitro
DOI:
https://doi.org/10.30659/odj.5.1.28-33Keywords:
Bioflm Streptococcus mutans, beluntas leaf extract 25Abstract
Background: Bioflm begins with formation of pelicle and within a minutes the colonization of bacteria attached to surface of the teeth. One of early bacteria attached was Streptococcus mutans. This study used 25% beluntas leaf extract in influencing the growth of Streptococcus mutans bioflm. The purpose of study was to investigated effect of 25% beluntas leaf extract on Streptococcus mutans bioflm.Methods: This research was experimental laboratory with post test only control design, consist of four treatment groups, 25% beluntas leaf extract and 0.12% chlorhexidine incubated 24 hours and 48 hours. Bioflm formation was measured by calculating Optical Density using a spectrophotometer. Data analysis was performed using One Way Anova test followed by Post Hoc LSD test.
Result: The results showed that beluntas leaf extract could influence the
formation of S.mutans bioflm but the effect in inhibiting bioflm formation is still not as good as chlorhexidine. It is known from the results of One Way Anova 25% beluntas leaf extract and 0.12% chlorhexidine incubated for 24 hours and 48 hours showed signifcant difference (p <0.05).
Conclusion: The conclusion of this research is the effect of beluntas leaf extract (Pluchea indica (L.) Less) 25% to Streptococcus mutans bioflm in vitro.
References
WHO[internet]. Switzerland: The world oral health report 2003 WHO global oral health programme.2003. [diakses 11 April 2017].
terdapat di: www.who.int/oral_health/media/en/ orh_report03_en.pdf
Ozdemir D. Dental caries : The most common disease worldwide and preventive strategies. Int J Biol. 2013;5(4):55.
Metwalli KH, Khan SA, Krom BP, Jabra-Rizk MA. Streptococcus mutans, Candida albicans, and the Human Mouth: A Sticky Situation. PLoS Pathog. 2013;9(10):1–5.
Karpinski TM, Szkaradkiewicz AK. Microbiology of dental caries. Med Rev J Biol Earth Sci J Biol Earth Sci. 2013;201(31):21–4.
Chetrus V, Ion IR. Dental plaque - classifcation, formation and identifcation. Int J Med Denstistry. 2013;3(2 April):139–43.
Morita A, Yulianto HDK, Kusdina SD, Purwanti N. Differences of Streptococcus mutans adhesion between artifcial mouth systems : a dinamic and static methods. Dent J. 2016;67(56):67–70.
Gurenlian JR. The rolse of dental plaque bioflm in oral health. J Dent Hyg. 2007;81(5):3–11.
Nahak MM, Tedjasulaksana R, Sumerti NN. Efektivitas kumur ekstrak etanol daun beluntas (pluchea indica. l.) untuk menurunkan jumlah koloni streptococcus sp. pada plak gigi. J Skala Husada. 2015;12(April):56–64.
Rahmi A, Cahyanto T, Sujarwo T, Lestari R indri. Uji aktivitas antibakteri ekstrak daun beluntas (Pluchea indica (L.) Less.) terhadap propionibacterium acnes penyebab jerawat. Fak Sains dan Teknol UIN Sunan Gunung Djati Bandung. 2015;IX(1):141–61.
Andriani JN. Reduction of Salivary Streptococcus mutans Colonies in Children After Rinsing with 2 . 5 % Green Tea Solution. J Dent Indones. 2014;21(3):81–6.
Putri RK, Habib I. Daya antifungi ekstrak etanol daun beluntas against Malassezia Sp . in vitro. Mutiara Med. 2007;7(1):7–17.
Nahak MM. Ekstrak etanol daun beluntas (Pluchea indica. L.) dapat menghambat pertumbuhan bakteri Streptococcus mutans
(Thesis). Udayana University; 2012.
Gunawan IWG, Bawa IGAG, Sutrisnayanti NL. Isolasi dan identifkasi senyawa terpenoid yang aktif antibakteri pada herba meniran (Phyllanthus niruri Linn). J Kim. 2008;2(1):31–9.
Sakinah N, Dwyana Z, Tambaru E, Rante H. Uji Aktivitas Sediaan Obat Kumur Ekstrak Daun Miana Coleus scutellarioides (L.) Benth Terhadap Pertumbuhan Bakteri Streptococcus
mutans. 2015;1–7.
Sinaredi BR, Pradopo S, Wibowo B. Daya antibakteri obat kumur chlorhexidine , povidone iodine , fluoride suplementasi zinc terhadap, Streptococcus mutans dan Porphyromonas gingivalis ( Antibacterial effect of mouth washes containing chlorhexidine , povidone iodine , fluoride plus zinc on. Dent J. 2014;47(4):212–4.
Downloads
Published
Issue
Section
License
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).

This work is licensed under a Creative Commons Attribution 4.0 International License.
