The Antibacterial Activity of Leaves Ethanol Extract, Stem Bark, and Latex of Angsana (Pterocarpus indicus Willd.) towards Bacterial Growth Streptococcus mutans as in vitro

Authors

DOI:

https://doi.org/10.30659/odj.5.1.1-8

Keywords:

Angsana leaves, Angsana stem bark, Angsana latex, Pterocarpus indicus Willd, antibacterial activity, Streptococcus mutans

Abstract

Background: Indonesia has many types of plants, which have medicinal properties and are used to cure various diseases. One of plant that has medicinal properties is the Angsana plant (Pterocarpus indicus Willd.) which traditionally can cure dental and oral diseases.
Method: This research used experimental laboratory with disc diffusion methods. Angsana (Pterocarpus indicus Willd.) was taken from as city ornamental tree in Samarinda city. The bacteria which used were Streptococcus mutans. The samples consisted of 11 treatment groups is leaves ethanol extract, stem bark, and Angsana latex respectively with concentration 25%, 50%, 75%, positive control group (chlorhexidine 0,2%), and negative control group (sterile aquades). Data analysis using Shapiro-Wilk test and One-Way Annova test.
Result: The results showed that leaves ethanol extract, stem bark, and Angsana latex (Pterocarpus indicus Willd.). All concentrations have antibacterial activity against the growth of Streptococcus mutans. The highest and the lowest concentrations that can inhibit the growth of Streptococcus mutans are 50% ethanol stem bark extract and 25% leaves ethanol extract.
Conclusion: The conclusion of this study proves that the leaves, stem bark ethanol extract, and Angsana latex (Pterocarpus indicus Willd.) Have antibacterial activity against the growth of Streptococcus mutans.

References

Iswandanis, W. Gambaran Pengetahuan Anak Usia 7 sampai dengan 12 Tahun tentang Oral Hygiene berdasarkan Karakteristik di SD Jalan Anyar Kota Bandung. Bandung: Universitas Pendidikan Indonesia; 2015.

Kristanti, Hapsari, D., & Pradono, J. Status Kesehatan Gigi dan Mulut di Indonesia. Analisis Data Survei Kesehatan Rumah tangga (SKRT). Jakarta: Badan Penelitian dan Pengembangan Kesehatan Department, Kesehatan Republik Indonesia; 2011.

Fejerskov, O., & Kidd, E. Dental Caries: The Disease and its Clinical Management (2nd ed.). Australia: Blackwell Munksgaard; 2008.

Isnarianti, R., Wahyudi, I., & Puspita, R. Muntingia calabura L Leaves Extract Inhibits Glucosyltransferase Activity of Streptococcus mutans. Journal of Dentistry Indonesia; 2013; 20(2); 59-63.

Badan Pengawas Obat dan Makanan Republik Indonesia. Formularium Ramuan Etnomedisin Obat Asli Indonesia (Vol. 3). Jakarta: Badan Pengawas Obat dan Makanan RI; 2013.

Biofarmaka IPB. Herbal Plants Collection Temulawak; 2013. Diunduh dari http://biofarmaka.ipb.ac.id/biofarmaka/2015/BCCS%20Herbal%20Plants%20Collections%20Temulawak.pdf

Krishanaveni, K., & Rao, J. An Isoflavone from Pterocarpus santalinus, Phytochemistry; 2000; 53.

Fatimah, C. Uji Aktivitas Antibakteri Ekstrak Daun Angsana (Pterocarpus indicus Willd.) secara In Vitro, Jurnal Ilmiah PANNMED (Pharmacist, Analyst, Nurse, Nutrition, Midwifery, Environment, Dentist); 2006; 1(1).

Gunawan, V. C. Analisis Kualitatif Dan Kuantitif Tanin pada Kulit Batang Angsana (Pterocarpus Indicus Willd). (Thesis, Universitas Ubaya, Surabaya); 2010. Diunduh dari http://digilib.ubaya.ac.id/pustaka.php/131832

Vinori, Y. Isolasi Terpenoid dari Kulit Tumbuhan Angsana (Pterocarpus Indicus Willd). (Skripsi tidak dipublikasikan). Universitas Negeri Padang, Padang; 2002.

Suwondo, S. Skrining Tumbuhan Obat yang Mempunyai Aktivitas Antibakteri Penyebab Karies Gigi dan Pembentuk Plak. Jurnal Bahan Alam Indonesia; 2007; 6 (2).

Clinical and Laboratory Standards Institute.. Performance Standards for Antimicrobial Disk Susceptibility Tests; Approved Standard—Eleventh Edition, CLSI Document M02-A11. Wayne: Author; 2012.

Jawetz, Melnick, & Adelberg. Mikrobiologi Kedokteran (25 ed.). (A. Adityputri, Penyunt., & A. W. Nugroho, Penerj.) Jakarta: EGC; 2012.

Suciati, A., Wardiyanto, & Sumino. Efektifitas Ekstrak Daun Rhizophora mucronata Dalam Menghambat Pertumbuhan Aeromonas salmonicida dan Vibrio harveyi. Jurnal Rekayasa dan Teknologi Budidaya Perairan; 2012; 1(1).

Elifah, E. Uji Antibakteri Fraksi Aktif Ekstrak Metanol Daun Senggani (Melastoma candidum D.Don) Terhadap Escherichia coli dan Bacillus subtilis serta Profil Kromatografi Lapis Tipisnya. (Skripsi tidak dipublikasikan). FMIPA Universitas Negeri Surakarta, Surakarta; 2010.

Syarif, N., & Panagan, T. A. Uji Daya Hambat Asap Cair Hasil Pirolisis Kayu Pelawan (Tristania abavata) Terhadap Bakteri Escherichia coli. Universitas Sriwijaya: Sumatera Selatan; 2009.

Sinarsih, N. K., Rita, W. S., Puspawati, N. M. Uji Efektifitas Ekstrak Daun Trembesi (Samanea saman (jacq.) Merr) Sebagai Antibakteri Escherichia coli dan Staphylococcus aureus. Journal of Applied Chemistry; 2016; 4(2).

Yulianti, R. Standardisasi Ekstrak Etanol Daun Angsana. Jakarta: UIN Syarifhidayatullah; 2013. Diunduh dari: http://repository.uinjkt.ac.id/dspace/bitstream/123456789/26472/1/RISDA%20YULIANTI-FKIK.pdf

Gunawan, A. W. I. Potensi Buah Pare (Momordica charantia L.) Sebagai Antibakteri Salmonella typhimurium. Skripsi. Denpasar: Program Studi Pendidikan Biologi, Fakultas keguruan dan Ilmu Pendidikan, Universitas Mahasaraswati Denpasar; 2009.

Susilowati, A., & Andi, B. Pengaruh Getah Tanaman jarak Pagar Jatropha curcas L) Terhadap Daya Hambat Bakteri Staphylococcus aureus Secara In Vitro. Skripsi. Semarang: Fakultas Peternakan, Universitas Diponegoro; 2014.

Karou, D., Savadogo, A., Canini, A., Yamego, S., Mantesano, C., Simpore, J., ...Traore. Antibacteria Activity of Alkaloids from Sida Acuta. African Journal of Biotechnology; 2006; 5(2); 195-200. Diunduh dari http://www.academicjournals.org/AJB.

Kurniawan, B., & Aryana, W.F. Binahong (Cassia alata L) as Inhibitor of Escherichia coli Growth. Majority Journal; 2015; 4(4); 100-104.

Darsana, I. G. O., Besung, I. N. K., dan Mahatmi, H. Potensi Daun Binahong (Anredera Cordifolia (Tenore) Steenis) dalam Menghambat Pertumbuhan Bakteri Escherichia Coli secara in vitro, Indonesia Medicus Veterinus; 2012; 1( 3); 337– 51.

Astarina, N. W. G., K. W. Astuti, N. K. Warditiani. Skrining fitokimia ekstrak metanol rimpang bangle (Zingiber purpureum Roxb.). 2013; Diunduh dari http://ojs.unud.ac.id/index.php/jfu/ article/download/7399/5649

Maftuhah, D., Bintari, S. H., Mustikaningtyas, D. Pengaruh Infusa Daun Beluntas (Pluchea indica) Terhadap Pertumbuhan Bakteri Staphylococcus epidermidis. Unnes Journal of Life Science; 2015; 4(1); 60-65. Diunduh dari http://journal.unnes.ac.id/sju/index.php/UnnesJLifeSci

Ismarani. Potensi Senyawa Tannin dalam Menunjang Produksi Ramah Lingkungan. Jurnal Agribisnis dan Pengembangan Wilayah. 2013; 3(2); 46-55.

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Published

2018-07-04

Issue

Vol. 5 No. 1 (2018): July 2018

Section

Original Article

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