THE INFLUENCE BETWEEN INJECTABLE PLATELET-RICH FIBRIN AND PLATELET-RICH PLASMA TOWARDS GINGIVAL FIBROBLAST CELL PROLIFERATION

Authors

DOI:

https://doi.org/10.30659/odj.8.2.25-31

Keywords:

Gingival fibroblast cell proliferation, Injectable platelet-rich fibrin, Platelet-rich plasma

Abstract

ABSTRACT

Background: Gingiva is the outermost periodontal tissue that acts as a mechanical and biological barrier to the root of the teeth and alveolar bone. The main cellular elements in the gingiva are fibroblasts. Fibroblast cell proliferation is an important process in tissue regeneration. Growth factors that can stimulate fibroblast cell proliferation can be found in regenerative agents, such as injectable platelet-rich fibrin (i-PRF) and platelet-rich plasma (PRP). The aim of this study was to examine the influence between i-PRF and PRP on the gingival fibroblast cell proliferation in vitro study on primary cell culture.
Method: Gingival fibroblast cell were obtained from primary cell culture derived from healthy gingiva. Ten mL of peripheral blood were centrifuged for i-PRF and PRP preparation. The samples were divided into three groups: i-PRF, PRP, and fibroblast cells without treatment. Cell proliferation were observed at day 1, day 3, day 5 using MTT assay at 550 nm. The data were analyzed by Two-Way ANOVA test, followed by Post Hoc test.
Result: The results showed that the cell proliferation increased from day 1, 3, and 5 in all groups. The absorbance value of the cell proliferation in order from highest to lowest: i-PRF, PRP, and cell control.
Conclusion: i-PRF and PRP increased the gingival fibroblast cell proliferation. i-PRF increased the cell proliferation higher than PRP.

Author Biographies

  • Arifia Anindita Danastri, Universitas Gadjah Mada
    Clinical Dentistry Program, Faculty of Dentistry
  • Suryono Suryono, Universitas Gadjah Mada
    Department of Periodontology, Faculty of Dentistry
  • Kwartarini Murdiastuti, Universitas Gadjah Mada
    Department of Periodontology, Faculty of Dentistry

References

Kinane DF, Stathopoulou PG, Papapanou PN. Periodontal Diseases. Nat. Rev. Dis. Primers. 2017; 3(17038): 1-14.

Han J, Menicanin D, Gronthos S, Bartold PM. Stem Cells, Tissue Engineering and Periodontal Regeneration. Aust Dent J. 2014; 59(SUPPL.1): 117-30.

Menicanin D, Hynes K, Han J, Gronthos S, Bartold PM. Cementum and Periodontal Ligament Regeneration. Adv Exp Med Biol. 2015; 881: 207-36.

Smith PC, Martinez C, Martinez J, McCulloch CA. Role of Fibroblasts Populations in Periodontal Wound Healing and Tissue Remodeling. Front Physiol. 2019; 10(270): 1-11.

Newman MG, Takei HH, Klokkevold PR, Carranza FA. Anatomy, Structure, and Function of the Periodontium. In: Fiorellini JP, Kim D, Chang Y, editors. Newman and Carranza’s Clinical Periodontology Thirteenth Edition. Philadelphia: Elsevier; 2019. p. 209-15.

Ardakani MRT, Hajizadeh F, Yadegari Z. Comparison of Attachment and Proliferation of Human Gingival Fibroblasts on Different Collagen Membranes. Ann Maxillofac Surg. 2018; 8(2): 218-23.

Suchetha A, Lalwani M, Darshan BM, Sapna N, Bhat D, Sravani K. Growth Factors: Role in Periodontal Regeneration. J Res Med Dent Sci. 2015; 3(3): 166-70.

Feigin K, Shope B. Use of Platelet-Rich Plasma and Platelet-Rich Fibrin in Dentistry and Oral Surgery: Introduction and Review of the Literature. J Vet Dent. 2019; 36(2): 109-23.

Kobayashi E, Fujioka-Kobayashi M, Sculean A, Chappuis V, Buser D, Schaller B, et al. Effects of Platelet-Rich Plasma (PRP) on Human Gingival Fibroblast, Osteoblast, and Periodontal Cell Ligament Behaviour. BMC Oral Health. 2017; 17(91): 1-10.

Miron RJ, Fujioka-Kobayashi M, Hernandez M, Kandalam U, Zhang Y, Ghanaati S, et al. Injectable Platelet-Rich Fibrin (i-PRF): Opportunities in Regenerative Dentistry?. Clin Oral Investig. 2017; 21(8): 2619-27.

Thanasrisuebwong P, Surarit R, Bencharit S, Ruangsawasdi N. Influence of Fractionation Methods on Physical and Biological Properties of Injectable Platelet-Rich Fibrin: An Exploratory Study. Int J Mol Sci. 2019; 20(7): 1-10.

Wang X, Zhang Y, Choukroun J, Ghanaati S, Miron RJ. Behavior of Gingival Fibroblasts on Titanium Implant Surfaces in Combination with either Injectable-PRF or PRP. Int J Mol Sci. 2017; 18(331): 1-15.

Choukroun J, Ghanaati S. Reduction of relative centrifugation force within injectable platelet-rich-fibrin (PRF) concentrates advances patients' own inflammatory cells, platelets and growth factors: the first introduction to the low speed centrifugation concept. Eur J Trauma Emerg Surg. 2018; 44(1): 87- 95.

Li X, Yang H, Zhang Z, Yan Z, Lv H, Zhang Y, et al. Platelet rich fibrin exudate promotes the proliferation and osteogenic differentiation of human periodontal ligament cells in vitro. Mol. Med. Rep. 2018; 18: 4477-85.

Nguyen PA, Pham TAV. Effects of platelet-rich plasma on human gingival fibroblast proliferation and migration in vitro. J Appl Oral Sci. 2018; 26: 1-9.

Wanichpakorn S, Kedjarune-Laggat U. Primary Cell Culture from Human Oral Tissue: Gingival Keratinocytes, Gingival Fibroblasts and Periodontal Ligament Fibroblasts. Songklanakarin J. Sci. Technol. 2010; 32(4): 327-31.

Vahabi S, Vaziri S, Torshabi M, Esfahrood ZR. Effects of Plasma Rich in Growth Factor Release and Platelet-Rich Fibrin on Proliferation and Viability of Human Gingival Fibroblasts. J Dent Tehran, 2015; 12(7): 504-12.

Izol BS, Uner DD. A New Approach for Root Surface Biomodification Using Injectable Platelet-Rich Fibrin (I-PRF). Med Sci Monit. 2019; 25: 4744-50.

Chandran P, Sivadas A. Platelet-Rich Fibrin: Its Role in Periodontal Regeneration, Saudi J Dent Res. 2014; 5(2): 117-22.

Varela HA, Souza JCM, Nascimento RM, Araujo RF, Vasconcelos RC, Cavalcante RS, et al. Injectable Platelet Rich Fibrin: Cell Content, Morphological, and Protein Characterization. Clin Oral Investig. 2019; 23(3): 1309-18.

Downloads

Published

2025-12-22

Issue

Vol. 8 No. 2 (2021): December 2021

Section

Original Article

License

Authors who publish with this journal agree to the following terms:

Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).

 

This work is licensed under a Creative Commons Attribution 4.0 International License.