Measurement of Pterygium Tissue Dry Weight Using Two Different Tissue Preparation Techniques in Freeze-Dry Method

Mohd Radzi Hilmi  -  Department of Optometry and Visual Sciences, Kulliyyah of Allied Health Sciences, International Islamic University Malaysia (IIUM)
Khairidzan Mohd Kamal*  -  Department of Ophthalmology, Kulliyyah of Medicine International Islamic University Malaysia (IIUM)
Mohd Zulfaezal Che Azemin  -  Department of Optometry and Visual Sciences, Kulliyyah of Allied Health Sciences, International Islamic University Malaysia (IIUM)
Azrin Esmady Ariffin  -  Faculty of Optometry and Vision Science, SEGi University, Petaling Jaya, Selangor, Malaysia

(*) Corresponding Author
DOI: 10.30659/sainsmed.v13i2.2958

Due to pterygium tissue compression on the corneal surface, it has been hypothesised that the degree of corneal astigmatism caused by pterygium can vary. This study aims to develop a freeze-dried method for estimating net pterygium tissue mass (NPTM) as dry weight. A single surgeon (KMK) excised 60 primary pterygium using the controlled partial avulsion technique and divided them into two groups: formalin-fixed (n=30) and unfixed (n=30). After determining the weight of each sterile container, 5 mL of 5% buffered formaldehyde was added to the formalin-fixed group and stored for one week, while 5 mL of distilled water was added to the non-fixed pterygium group. Each container was pre-frozen for 12 hours before being freeze-dried (-24 hours at -80 degrees Celsius). The result is referred to as the net pterygium tissue mass (NPTM). Using an independent T-test, a comparison of wet and dry weight and percentage of NPTM was conducted between groups. Wet weights for formalin-fixed and non-fixed pterygium were 253.33 82.17 g and 255.17 63.52 g, respectively, while dry weights were 184.92 84.31 g and 179.54 72.85 g. Formalin-fixed pterygium tissue revealed a slightly higher percentage of NPTM than non-fixed pterygium tissue (69.39 13.29% vs. 67.75 13.29%, p = 0.792), but this difference was not statistically significant. The freeze-dried method can be utilised to quantify the NPTM of pterygium fibrovascular tissue and investigate the influence of pterygium translucency on predicting induced-corneal astigmatism.

Keywords: Pterygium; Freeze-dried; Dry weight; Corneal astigmatism

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DOI : 10.30659/sainsmed

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