ALUMINUM CHLORIDE IMPAIRED SPATIAL MEMORY, BUT NOT SENILE PLAQUES FORMATION IN THE RAT MODEL OF ALZHEIMER’S DISEASE

Muhammad Ihwan Narwanto*  -  Department of Anatomy, Faculty of Medicine, University of Jember, Jember, Indonesia., Indonesia

(*) Corresponding Author

Background: aluminum compounds can be easily found in the environment. Aluminum contamination is the environmental factor as one of the risk factors in Alzheimer's disease (AD). In the animal model, aluminum chloride (AlCl3) causes inflammation and oxidative stress. Inflammation and oxidative stress are important pathogenesis pathways in AD. Objective: This study is to investigate whether AlCl3 can impair spatial memory and induce senile plaques formation.

Methods: we used 24 young adult Wistar rats in this study. The rats were divided into four groups; one control group and three AlCl3 treated groups with doses of 150 mg/kg, 300 mg/kg, and 600 mg/kg, respectively for 8 weeks. The spatial memory test was measured using Morris water maze and the histopathology was done by identification of senile plaques formation in the hippocampal tissue.

Results: we showed that there are significant differences between the control group and all of the AlCl3 treatment groups in the memory test, however, there is no change in the senile plaques expression in all groups.

Conclusion: in this study, we demonstrated that administration of AlCl3 for 8 weeks can cause the impaired of spatial memory without senile plaques formation.

Keywords : aluminum chloride, spatial memory, senile plaques, Alzheimer’s disease

  1. Agostinho, P., Cunha, R. a and Oliveira, C. (2010) ‘Neuroinflammation, Oxidative Stress and the Pathogenesis of Alzheimer ’ s Disease’, Current Pharmacutical Design, 16, pp. 2766–2778.
  2. Aguilar, F, Autrup H, Barlow S, Castle L, Crebelli R, Dekant W, et al. (2008) ‘Safety of aluminium from dietary intake - Scientific Opinion of the Panel on Food Additives, Flavourings, Processing Aids and Food Contact Materials (AFC)’, EFSA Journal, 6(7), pp. 1–34. doi: 10.2903/j.efsa.2008.754.
  3. Baydar T, Papp A, Aydin A, Nagymajtenyi L, Schulz H, Isimer A, et al. (2003) ‘Accumulation of aluminum in rat brain’, Biological Trace Element Research, 92(3), pp. 231–244. Available at: http://dx.doi.org/10.1385/BTER:92:3:231.
  4. Bromley-Brits K, Deng Y, Song W. (2011) Morris Water Maze test for learning and memory deficits in Alzheimer’s disease model mice. J Vis Exp.(53, pp. 2–6.
  5. Cao Z, Yang X, Zhang H, Wang H, Huang W, Xu F, et al. (2016) ‘Aluminum chloride induces neuroinflammation, loss of neuronal dendritic spine and cognition impairment in developing rat’, Chemosphere. Elsevier Ltd, 151, pp. 289–295. doi: 10.1016/j.chemosphere.2016.02.092.
  6. Chen SM, Fan CC, Chiue MS, Chou C, Chen JH, Hseu RS. (2013) ‘Hemodynamic and neuropathological analysis in rats with aluminum trichloride-induced Alzheimer’s disease’, PLoS ONE, 8(12), pp. 1–11. doi: 10.1371/journal.pone.0082561.
  7. Deture, M. A. and Dickson, D. W. (2019) ‘The neuropathological diagnosis of Alzheimer’s disease’, Molecular Neurodegeneration. Molecular Neurodegeneration, 14(1), pp. 1–18. doi: 10.1186/s13024-019-0333-5.
  8. Jayakar JP, Huang J. (2010) Diagnostic review Alzheimer’s disease : A review of diagnostic criteria. Univ West Ont Med J. 79(2), pp.7–9.
  9. Killin LOJ, Starr JM, Shiue IJ, Russ TC. (2016) Environmental risk factors for dementia: a systematic review. BMC Geriatr.16(1), pp.1–28. http://dx.doi.org/10.1186/s12877-016-0342-y
  10. Lee J-E, Han P-L. (2013) An Update of Animal Models of Alzheimer Disease with a Reevaluation of Plaque Depositions. Exp Neurobiol.22(2), pp.84–95.
  11. Lin WT, Chen RC, Lu WW, Liu SH, Yang FY. (2015) ‘Protective effects of low-intensity pulsed ultrasound on aluminum-induced cerebral damage in Alzheimer’s disease rat model’, Scientific Reports, 5, pp. 1–7. doi: 10.1038/srep09671.
  12. Nobakht M, Hoseini SM, Mortazavi P, Sohrabi I, Esmaeilzade B, Rooshandel NR, et al. (2011) Neuropathological changes in brain cortex and hippocampus in a rat model of Alzheimer’s disease. Iran Biomed J.15(1–2) pp.51–8.
  13. Rajamohamedsait, H. B. and Sigurdsson, E. M. (2012) ‘Histological staining of amyloid and pre-amyloid peptides and proteins in mouse tissue’, Methods in Molecular Biology, 849, pp. 411–424. doi: 10.1007/978-1-61779-551-0_28.
  14. Rondeau V, Jacqmin-Gadda H, Commenges D, Helmer C, Dartigues JF. (2009) ‘Aluminum and silica in drinking water and the risk of Alzheimer’s disease or cognitive decline: Findings from 15-year follow-up of the PAQUID cohort’, American Journal of Epidemiology, 169(4), pp. 489–496. doi: 10.1093/aje/kwn348.
  15. Wall AM, Mukandala G, Greig NH, Sciences B, Branch G. (2016) ‘HHS Public Access’, 93(5), pp. 815–829. doi: 10.1002/jnr.23540.
  16. Wenk GL, Mcgann K, Hauss-Wegrzyniak B, Rosi S. (2003) ‘The toxicity of tumor necrosis factor-α upon cholinergic neurons within the nucleus basalis and the role of norepinephrine in the regulation of inflammation: Implications for Alzheimer’s disease’, Neuroscience, 121(3), pp. 719–729. doi: 10.1016/S0306-4522(03)00545-1.
  17. Yuan, C. Y., Lee, Y. J. and Hsu, G. S. W. (2012) ‘Aluminum overload increases oxidative stress in four functional brain areas of neonatal rats’, Journal of Biomedical Science, 19(1), pp. 1–9. doi: 10.1186/1423-0127-19-51.

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ISSN: 2339-093X (Online) | 2085-1545 (Print)
DOI : 10.30659/sainsmed

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