No Structural Change in Mice Intestinal Gut-Associated Lymphoid Tissue After Trichuris muris Egg Infestation
Introduction: It has been known that the infestation of Trichuris muris egg in the intestinal mice may induce a host immune response to eliminate the worms. Worms antigen will stimulate host immune response in the intestinal secondary lymphoid organs. One of the secondary lymphoid organs in the intestinum is GALT (gut-associated lymphoid tissue). Worm antigen activate naive T cells and naive B cells in GALT and trigger differentiation of T cells into Th2 and stimulate B cell to produce gut IgE. However a structural change of GALT structure is still poorly understood. Objective: This study aims to find influence of embrionic Trichuris muris egg infestation to the structural changes of mice intestinum GALT.
Methods: This research uses true experimental method with post-test only with control group design. Mice are grouped into 3 groups. The first group was given 40 Trichuris muris embryonic egg peroral, second group was given 200 eggs, and third was control group. On the 30th day after treatment, the mice were sacrificed and intestinal GALT structural change has been analyzed (p<0.05).
Results: There was no effect of Trichuris muris embryonic egg infestation on the structure of mice intestinum GALT. However, there is a unique finding of GALT in the form of a Peyer’s patch in the basal plica transversalis colon proximalis of 1 mice given egg T. peroral murmus, at low doses or high doses.
Conclusion: There was no effect of Trichuris muris embryonic egg infestation on the structure of mice intestinum GALT
Keywords : GALT; Peyer’s patch; Trichuris muris; sel Th2; sel B
- Antignano, F., Mullaly, S. C., Burrows, K. & Zaph, C. 2011. Trichuris muris infection: a model of type 2 immunity and inflammation in the gut. J Vis Exp.
- Bao, K. & Reinhardt, R. L. 2015. The differential expression of IL-4 and IL-13 and its impact on type-2 immunity. Cytokine, 75, 25-37.
- Cornes, J. S. 1965. Number, size, and distribution of Peyer's patches in the human small intestine: Part I The development of Peyer's patches. Gut, 6, 225-9.
- Elieh Ali Komi, D., Sharma, L. & Dela Cruz, C. S. 2018. Chitin and Its Effects on Inflammatory and Immune Responses. Clin Rev Allergy Immunol, 54, 213-223.
- Gilbert, J. A., Blaser, M. J., Caporaso, J. G., Jansson, J. K., Lynch, S. V. & Knight, R. 2018. Current understanding of the human microbiome. Nature Medicine, 24, 392-400.
- Granger, D. N., Grisham, M. B. & Kevil, C. G. 2006. CHAPTER 46 - Recruitment of Inflammatory and Immune Cells in the Gut: Physiology and Pathophysiology. In: JOHNSON, L. R. (ed.) Physiology of the Gastrointestinal Tract (Fourth Edition). Burlington: Academic Press.
- Holm, J. B., Sorobetea, D., Kiilerich, P., Ramayo-Caldas, Y., Estellé, J., Ma, T., Madsen, L., Kristiansen, K. & Svensson-Frej, M. 2015. Chronic Trichuris muris Infection Decreases Diversity of the Intestinal Microbiota and Concomitantly Increases the Abundance of Lactobacilli. PLOS ONE, 10, e0125495.
- Houlden, A., Hayes, K. S., Bancroft, A. J., Worthington, J. J., Wang, P., Grencis, R. K. & Roberts, I. S. 2015. Chronic Trichuris muris Infection in C57BL/6 Mice Causes Significant Changes in Host Microbiota and Metabolome: Effects Reversed by Pathogen Clearance. PLOS ONE, 10, e0125945.
- Kato, LM., Kawamoto, S., Maruya, M., Fagarasan S. 2014. The role of the adaptive immune system in regulation of gut microbiota. Immunol Rev. 260(1):67-75. doi: 10.1111/imr.12185. PMID: 24942682.
- Kimura, S. 2018. Molecular insights into the mechanisms of M-cell differentiation and transcytosis in the mucosa-associated lymphoid tissues. Anat Sci Int, 93, 23-34.
- Klementowicz, J. E., Travis, M. A. & Grencis, R. K. 2012. Trichuris muris: a model of gastrointestinal parasite infection. Semin Immunopathol, 34, 815-28.
- Kong, S., Zhang, Y. H. & Zhang, W. 2018. Regulation of Intestinal Epithelial Cells Properties and Functions by Amino Acids. BioMed research international, 2018, 2819154-2819154.
- Linden, M., Ward, J. M. & Cherian, S. 2012. 19 - Hematopoietic and Lymphoid Tissues. In: TREUTING, P. M. & DINTZIS, S. M. (eds.) Comparative Anatomy and Histology. San Diego: Academic Press.
- Meier, DG., Docena, H., Ramisch, D., Toscanini, U., Berardi, G., Gondolesi, GE., M. Rumbo, M. 2014. Immunological Status of Isolated Lymphoid Follicles After Intestinal Transplantation, American Journal of Transplantation, 10.1111/ajt.12797, 14, 9, (2148-2158), (2014).
- Mestecky, J., Strober, W., Russel, M., BL, B. K., Cheroutre, H. & Volume, B. L. 2015. Mucosal Immunology USA, Elsevier
- Mörbe, U. M., Jørgensen, P. B., Fenton, T. M., von Burg, N., Riis, L. B., Spencer, J. & Agace, W. W. 2021. Human gut-associated lymphoid tissues (GALT); diversity, structure, and function. Mucosal Immunology.
- Nochi, T., Denton, P. W., Wahl, A. & Garcia, J. V. 2013. Cryptopatches are essential for the development of human GALT. Cell Rep, 3, 1874-84.
- Senda, T., Dogra, P., Granot, T., Furuhashi, K., Snyder, M. E., Carpenter, D. J., Szabo, P. A., Thapa, P., Miron, M. & Farber, D. L. 2019. Microanatomical dissection of human intestinal T-cell immunity reveals site-specific changes in gut-associated lymphoid tissues over life. Mucosal Immunology, 12, 378-389.
- Stagg, A. J. 2018. Intestinal Dendritic Cells in Health and Gut Inflammation. Frontiers in Immunology, 9.
- Stahl, M. G. & Belkind-Gerson, J. 2021. 1 - Development of the Gastrointestinal Tract. In: WYLLIE, R., HYAMS, J. S. & KAY, M. (eds.) Pediatric Gastrointestinal and Liver Disease (Sixth Edition). Philadelphia: Elsevier.
- Viswanath, A., Yarrarapu, S. N. S. & Williams, M. 2021. Trichuris Trichiura. StatPearls. Treasure Island (FL): StatPearls Publishing.
- Zheng, D., Liwinski, T. & Elinav, E. 2020. Interaction between microbiota and immunity in health and disease. Cell Research, 30, 492-506.