Alfa Mangostin Extract (Garcinia Mangostana L) Effectiveness on the Biofilm Thickness Growth of Streptococcus Sanguinis.

Rahmawati Sri Praptiningsih, Rosa Pratiwi, tazkia Yustisiani Nurafifah

Abstract


Background: Periodontal disease is caused by the accumulation of bacteria from biofilms in the periodontal tissues. Streptococcus sanguinis is one of bacteria lives in biofilm. One of the herbal ingredients that can be used as an antibacterial is the mangosteen fruit (Garcinia mangostana L.), especially the rind of the mangosteen fruit. The main compound in the mangosteen rind is known as alfa-mangostin, the compound alfa-mangostin has anti-bacterial benefits. The purpose of this research is to determine the effectiveness usage of alpha mangostin toward Streptococcus sanguinis biofilm thickness growth.
Method: Experimental research design, with post-test only control group design with a sample size of 27. The treatment group consisted of alpha mangostin 3.125, 6.25, and 12.5 g/ml and the control group were distilled water. Measurement of Streptococcus sanguinis biofilm thickness was carried out by ELISA-reader. The statistical test was carried out using the One Way Anova test.
Results: The results showed the lowest average thickness of Streptococcus sanguinis with the addition of -mangostin 12.5 g/ml was 0.371, while the thickness of Streptococcus sanguinis with the addition of chlorhexidine was the highest at 1.860. One Way Anova test obtained a significance number of 0.001 (p <0.05) so it can be concluded that there are significant differences in
the 4 groups of Streptococcus sanguinis.
Conclusion: Alpha mangostin has antibacterial properties that can reduce the thickness of the Streptococcus sanguinis biofilm.

Keywords


alpha mangostin, biofilm thickness, Streptococcus sanguinis

Full Text:

PDF

References


REFERENCES

Zakki, M. (2017) ‘Uji Aktivitas Antibakteri Ekstrak Cathechin Teh Putih Terhadap’, Odonto Dental Journal, 4(2), Pp. 108–113.

Academy, T. A. et al. (2015) ‘American Academy of Periodontology Task Force Report on the Update to the 1999 Classification of Periodontal Diseases and Conditions’, Journal of Periodontology, 86(7), pp. 835–838. doi: 10.1902/jop.2015.157001.

Ahyar Afiana; Rakhmawatie, Maya Dian, F. W. R. (2017) ‘in Vitro Effect of Alfa Mangostin on Multiresistant Uropathogenic Escherichia Coli’, Sains Medika: Jurnal Kedokteran dan Kesehatan, 8(Vol 8, No 1 (2017): January-June 2017), pp. 20–27. Available at: http://jurnal.unissula.ac.id/index.php/sainsmedika/article/view/1002.

Amanda, E. A., Oktiani, B. W. and Panjaitan, F. U. (2019) ‘Efektivitas Antibakteri Ekstrak Flavonoid Propolis trigona sp (Trigona thorasica) Terhadap Pertumbuhan Bakteri Porphyromonas gingivalis’, Dentin Jurnal Kedokteran Gigi, 3(1), pp. 23–28.

Arini, N. W., Astuti, S. A. P. D. and Nahak, M. M. (2014) ‘EFEKTIVITAS KUMUR-KUMUR AIR REBUSAN KULIT BUAH MANGGIS PASCA ORAL FISIOTERAPI UNTUK PENYEMBUHAN GINGIVITIS Ni Wayan Arini 1 , Sagung Agung Putri Dwi Astuti 2 , Maria Martina Nahak 3’, Jurnal Skala Husada, 11(1), pp. 6–10.

Attamimi, F. A., Ruslami, R. and Maskoen, A. M. (2017) ‘Uji Aktivitas Antibakteri Ekstrak Kasar Umbi Sarang Semut (Myrmecodia pendens) Dibanding dengan Klorheksidin terhadap Streptococcus sanguinis’, Majalah Kedokteran Bandung, 49(2), pp. 94–101. doi: 10.15395/mkb.v49n2.1053.

Azeredo, J. et al. (2017) ‘Critical review on biofilm methods’, Critical Reviews in Microbiology, 43(3), pp. 313–351. doi: 10.1080/1040841X.2016.1208146.

Basri, Fina Maghfirah, D. S. (2016) ‘Aktivitas Pembentukan Biofilm Streptococcus Mutans dan Candida Albicans setelah dipapar Dengan Cigarette Smoke Condensate dan Minuman Probiotik’, 2(July), pp. 1–23.

Bin, Z. et al. (2018) ‘Streptococcus Sanguinis Biofilm Formation & Interaction with Oral Pathogens’, Future Microbiology, 13(8), pp. 915–932. Available at: http://www.embase.com/search/results?subaction=viewrecord&from=export&id=L622901472%0Ahttp://dx.doi.org/10.2217/fmb-2018-0043.

Darmawansyih (2014) ‘Khasiat Buah Manggis untuk Kehidupan’, Jurnal Al Hikmah, XV, pp. 60–68. Available at: https://media.neliti.com/media/publications/30612-ID-khasiat-buah-manggis-untuk-kehidupan.pdf.

Egi, M, et al (2018) ‘Efek Berkumur Sari Buah Tomat ( Solanum lycopersicum’, Sound of Dentistry, 3(2), pp. 70–84.

García-huerta, O. E. et al. (2012) ‘Periodontal Diseases - A Clinician’s Guide’, Periodontal Diseases - A Clinician’s Guide. doi: 10.5772/1274.

Hamsar, A. and Ramadhan, E. S. (2019) ‘Penggunaan Chlorhexidine Kumur dalam Perbaikan Indeks Kebersihan Gigi Pegawai Poltekkes Kemenkes RI Medan’, Jurnal Kesehatan Gigi, 6(2), pp. 99–103. doi: 10.31983/jkg.v6i2.5482.

Kemala, D., Hendiani, I. and Satari, M. H. (2018) ‘Uji daya antibakteri ekstrak etanol kulit buah manggis (Garcinia mangostana L) terhadap Streptococcus sanguinis ATCC 10556’, Padjadjaran Journal of Dental Researchers and Students, 2(2), p. 137. doi: 10.24198/pjdrs.v3i1.21447.

Kementerian Kesehatan Republik Indonesia (2018) ‘Laporan Nasional Riset Kesehatan Dasar’, Kementrian kesehatan RI, pp. 1–582. Available at: https://dinkes.kalbarprov.go.id/wp-content/uploads/2019/03/Laporan-Riskesdas-2018-Nasional.pdf.

Kwartiningsih, E. et al. (2009) ‘Zat Pewarna Alami Tekstil Dari Kulit Buah Manggis’, Ekuilibrium, 8(1), pp. 41–47.

Lumentut, R. A. N., Gunawan, P. N. and Mintjelungan, C. N. (2013) ‘Status Periodontal Dan Kebutuhan Perawatan Pada Usia Lanjut’, e-GIGI, 1(2), pp. 79–83. doi: 10.35790/eg.1.2.2013.2619.

Mirawati, E. (2014) ‘Efektivitas Obat Kumur Yang Mengandung Cengkeh dan Clorhexidine Gluconat 0,2% Dalam Pencegahan Pembentukan Plak’, Paper Knowledge . Toward a Media History of Documents, 16(2), pp. 34–39.

Nazir, M. et al. (2020) ‘Global Prevalence of Periodontal Disease and Lack of Its Surveillance’, Scientific World Journal, 2020. doi: 10.1155/2020/2146160.

Newman, M. G. and Carranza, F. (2006) Clinical Periodontology 10o Edition, Saunders Elsevier.

Nguyen, P. T. M. et al. (2014) ‘Α-Mangostin Disrupts the Development of Streptococcus Mutans Biofilms and Facilitates Its Mechanical Removal’, PLoS ONE, 9(10). doi: 10.1371/journal.pone.0111312.

Nisa, T. D. and Primartha, R. (2013) ‘Diagnosis Penyakit Gigi Periodontal Menggunakan Sistem Pakar Fuzzy’, Jurnal Generic, x(x), p. x ~ x. Available at: http://eprints.unsri.ac.id/4122/1/diagnosa_penyakit_gigi_periodontal.pdf.

Norhayati, N. et al. (2019) ‘ANTIBACTERIAL POTENTIAL OF KAPUL FRUIT SKIN (Baccaurea macrocarpa) ON Streptococcus sanguis’, ODONTO : Dental Journal, 6(2), p. 118. doi: 10.30659/odj.6.2.118-124.

Patogen, B., Primasari, V. S. and Ramadhani, A. R. (2021) ‘POTENSI MINYAK ESENSIAL KAYU MANIS ( Cinnamomum’, 1(2).

Pelczar, M. J. and Chan (2008) Dasar-dasar Mikrobiologi jilid 1. 1st edn. Edited by Ratna Siri Hadioetomo. jakarta: Universitas Indonesia.

Pratiwi, A. R., Hendiani, I. and Pribadi, I. M. S. (2016) ‘

Perbandingan berkumur larutan ekstrak kulit buah manggis dan Enkasari® terhadap penurunan indeks plak

Comparison of gargling solution of mangosteen pericarp extract and Enkasari® in decreasing plaque index

’, Jurnal Kedokteran Gigi Universitas Padjadjaran, 28(3). doi: 10.24198/jkg.v28i3.18696.

Pujiastuti, P. et al. (2012) ‘Obesitas dan penyakit periodontal’, Stomagtonatic, 9(2), pp. 82–85.

Ramadhani, Z. F., Putri, D. K. T. and Cholil (2014) ‘Prevalensi penyakit periodontal pada perokok di lingkungan batalyon infanteri 621/manuntung barabai hulu sungai tengah’, Dentino jurnal kedokteran gigi, II(2), p. 116.

Rubiyanti, R., Susilawati, Y. and Muchtaridi, M. (2016) ‘Potensi Ekonomi dan Manfaat Kandungan Alfa-Mangostin Serta Gartanin dalam Kulit Buah Manggis (Garcinia mangostana Linn)’, Farmaka, 15(1), pp. 15–25.

Santoso, A. P. R. and Wulandari, D. D. (2020) ‘Pengaruh Pemberian Alfa Mangostin Terhadap Kadar Glukosa Pada Tikus Yang Diinduksi Diet Tinggi Fruktosa’, Medical Technology and Public Health Journal, 4(2), pp. 127–133. doi: 10.33086/mtphj.v4i2.1710.

Skogman, M. E., Vuorela, P. M. and Fallarero, A. (2012) ‘Combining biofilm matrix measurements with biomass and viability assays in susceptibility assessments of antimicrobials against Staphylococcus aureus biofilms’, Journal of Antibiotics, 65(9), pp. 453–459. doi: 10.1038/ja.2012.49.

Soegiharto, C., Pratiwi, R. and Wardhana, E. S. (2022) ‘Efektivitas Nanoemulsi Gel Daun Pegagan ( Centella Asiatica L . ) Konsentrasi 25 %, 50 % Dan 75 % Terhadap Ketebalan Biofilm Bakteri Staphylococcus Aureus ( In Vitro )’, 110, pp. 94–101.

Srihari, E. and Lingganingrum, F. S. (2015) ‘Ekstrak Kulit Manggis Bubuk’, Jurnal Teknik Kimia, 10(1), pp. 1–7.

Sumiati, T., Eem, M. and Khamim, N. M. (2021) (2021) ‘Formulasi Obat Kumur Herbal Ekstrak Etanol Kulit Buah Nanas (Ananas comosus (L.) Sebagai Antibakteri Streptococcus sanguinis Penyebab Plak Gigi’, Jurnal Farmamedika (Pharmamedica Journal), 6(1), pp. 15–23. doi: 10.47219/ath.v6i1.112.

Syaravina, C. B., Amalina, R. and Hadianto, E. (2018) ‘PENGARUH EKSTRAK DAUN BELUNTAS (Pluchea indica (L.) Less) 25% TERHADAP BIOFILM STREPTOCOCCUS MUTANS - in vitro’, ODONTO : Dental Journal, 5(1), p. 28. doi: 10.30659/odj.5.1.28-33.

Wahyuni, S. and Wijaya, W. P. (2019) ‘Pengaruh Penambahan Bahan Kompatibilisasi Pada Nilon Daur Ulang Terhadap Kekuatan Fleksural Basis Gigi Tiruan Nilon Termoplastik’, B-Dent: Jurnal Kedokteran Gigi Universitas Baiturrahmah, 6(1), pp. 42–48. doi: 10.33854/jbd.v6i1.129.

Yanura, A. (2016) ‘POTENSI α-MANGOSTIN DARI EKSTRAK PERIKARP MANGGIS (Garcinia mangostana L.) DALAM MENGHAMBAT SEKRESI PROTEIN CULTURE FILTRATE PROTEIN (CFP)-10 PADA Mycobacterium tuberculosis H37Rv’, Pharmaceutical Journal of Indonesia, 2(1), pp. 12–17. doi: 10.21776/ub.pji.2016.002.01.3.

Yulianti Kemal, Robert Lesang, Natalina, Boy Muchlis Bachtiar, L. H. M. (2012) ‘Analisis Morfologi Koloni dan Keberagaman Genotip Streptococcus sangunis Yang Berasal Dari Plak Gigi Dan Saliva Penderita Penyakit Jantung Koroner’, analisi genotip STREPTOCOCCUS SANGUINIS YANG BERASAL DARI PLAK GIGI DAN SALIVA PENDERITA PENYAKIT JANTUNG KORONER, 17, pp. 153–156.

Yusran, A. and Muhasbir, M. (2018) ‘Daya Hambat Minyak Kelapa Murni Terhadap Pertumbuhan Bakteri Streptococcus sanguinis’, Makassar Dental Journal, 7(3), pp. 146–150.

Zakki, M. (2017a) ‘Uji Aktivitas Antibakteri Ekstrak Cathechin Teh Putih Terhadap’, ODONTO Dental Journal, 4(2), pp. 108–113.

Zakki, M. (2017b) ‘Uji Aktivitas Antibakteri Ekstrak Cathechin Teh Putih Terhadap Streptococcus Sanguinis’, ODONTO : Dental Journal, 4(2), p. 108. doi: 10.30659/odj.4.2.108-113.

Suratri, M. A. L. (2020) ‘Pengaruh Hipertensi Terhadap Kejadian Penyakit Jaringan Periodontal (Periodontitis) pada Masyarakat Indonesia (Data Riskesdas 2018)’, Buletin Penelitian Kesehatan, 48(4), pp. 227–234. doi: 10.22435/bpk.v48i4.3516.




DOI: http://dx.doi.org/10.30659/odj.10.0.7-14

Refbacks

  • There are currently no refbacks.


Lisensi Creative Commons
This work is licensed under a Lisensi Creative Commons Atribusi-BerbagiSerupa 4.0 Internasional.
Contact us: Odonto Dental Journal: Jl. Raya Kaligawe Km.4, PO BOX 1054/SM Semarang, Central Java, Indonesia, 50112. Email: odontodentaljournal@unissula.ac.id