PERBANDINGAN EFEKTIFITAS JUS LIDAH BUAYA DAN KLORHEXIDIN 0.06% TERHADAP JUMLAH KOLONI STREPTOCOCCUS MUTANS SALIVA ANAK DENGAN KARIES

Shanty Chairani, Sri W Rais, Rani Purba, Amalia AH

Abstract


Background: Aloe vera contains active components such as antrhaquinones,
saponin, glucomannans, and acemannans that have been known for its antimicrobial properties. In vitro study showed that Aloe vera extract can inhibit the growth of Streptococcus mutans. Studies reported that children with caries have high level of salivary S. mutans which is the main pathogen in dental caries. The gold standard mouthrinse for dental caries is chlorhexidine gluconate, but it has several side effects. The objective of this study was to compare the effect between Aloe vera juice and 0.06% chlorhexidine mouth rinse on salivary S. mutans in children with caries.
Methods: This experimental research with pretest and post-test control group design conducted on 30 children (6-12 years old) with number of carious teeth >4. Subjects divided into 2 groups: Aloe vera juice group and 0.06% chlorhexidine group, which instructed to rinse their mouth with 10 ml stated mouthwash, twice a day, during 7-day period. Unstimulated saliva was collected at baseline and after 7 days then inoculated on mitis salivarius bacitracin agar. S. mutans were counted and expressed in CFU/ml. Data were analyzed using paired t-test and independent t-test.
Result: The result showed that a significant reduction in salivary S.mutans
counts was observed with Aloe vera juice mouth rinse and 0.06% chlorhexidine (p<0.05). The mean reduction of S. mutans counts was not statistically significantly between grup (p>0.05).
Conclusion: These findings suggest that Aloe vera juice can reduce salivary S. mutans in children with caries so it can be considered as alternative mouthwash to prevent caries.

Keywords


Aloe vera juice; chlorhexidine; mouth rinse; Streptococcus mutans; saliva

Full Text:

PDF

References


Wahjono E, Koesnandar. Mengebunkan lidah buaya secara intensif. Jakarta: Agromedia Pustaka. 2002. p. 2-12.

Surjushe A, Vasani R, Saple DG. Aloe vera: a short review. Indian J Dermatol. 2008; 53(4): 163-6.

Basetti A, Sala S. The great aloe book. USA: Zuccani. 2005. p. 46-56.

Nonong YH, Mieke HS, Ratna I, Selly P. Antibacterial test between Aloe vera and chlorhexidine based on the number of colony of Streptococcus mutans ATCC 25 175 in vitro. IJSR. 2016; 5(1):1379-85.

Meyer-Lueckel H, Paris S, Ekstrand KR. Caries management—science and clinical practice. USA: Thieme; 2013. p. 10-12, 22, 28-32.

Nishimura J, Saito T, Yoneyama H, Bai LL, Okumura K, Isogai E. Biofilm formation by Streptococcus mutans and related bacteria. Advances in Microbiology. 2012; 2: 208-15.

Parampreet P, Ramandeep G, Amrita S. Correlation between the salivary Streptococcus mutans levels and dental caries experience in adult population of Chandigarh, India. European

Journal of Dentistry. 2013; 7(2): 191-5.

Ja’far ZJ, Al-Bayati YAA, Taha GI. Correlation between caries related bacteria in plaque and saliva in different age group children. J Bagh College Dentistry. 2012; 24(3): 140-4.

Nanda J, Scahdev V, Sandhu M, Nanda KDP. Correlation between dental caries experience and mutans streptococci counts using saliva and plaque as microbial risk indicators in 3-8 year old children. A cross sectional study.J Clin Exp Dent. 2015; 7(1):e114-8.

Badan Penelitian dan Pengembangan Kesehatan Kementerian Kesehatan RI. Riskesdas 2013 dalam angka; 2013. p. 215-6.

Ramayanti S, Purnakarya I. Peran makanan terhadap karies gigi. Jurnal Kesehatan Masyarakat. 2013; 7(2): 89-93.

Shruti B, Radhika A. Chlorhexidine: the gold standard antiplaque agent.J Pharm Sci & Res. 2013; 5(12): 270-4.

Nonong YH, Mieke HS, Ratna I, Selly P. Antibacterial test between Aloe vera and chlorhexidine based on the number of colony of Streptococcus mutans ATCC 25 175 in vitro. IJSR. 2016; 5(1):1379-85.

Jayaprakash R, Sharma A, Moses J. Comparative evaluation of the efficacy of different concentrations of chlorhexidine mouth rinses in reducing the mutans streptococci in saliva: An in vivo study. 2010; 28(3): 162-6.

Kumar GR, Gupta D, Bhaskar DJ, Yadav A, Obaid K, Mishra S. Preliminary antiplaque efficacy of Aloe vera mouthwash on 4 day plaque re-growth model: randomized control trial. Ethiop J Health

Sci. 2014; 24(2): 139-44.

Nandan N, Mythri P, Prem KK. Effect of green tea as a mouth rinse on Streptococcus mutans. Journal of Ayurveda and Integrated Medical Sciences. 2016; 1(1): 7-11.

Sajadi FS, Moradi M, Pardakhty A, Yazsizadeh R, Madani F. Effect of fluoride, chlorhexidine and fluoride-chlorhexidine mouthwashes on salivary Streptococcus mutans count and the prevalence of oral side effects. JODDD. 2015; 9(1): 49.52.

Pankaj KS, Deen DG, Ritu S, Priyanka P, Sharmistha G, Atul KS, et al. Therapeutic and medicinal uses of Aloe vera: a review.

Pharmacology & Pharmacy. 2013; 4: 599-610.

Subramiam P, Dwivedi S. Uma E, Girish BKL. Effect of pomegranate and Aloe vera extract on Streptococcus mutans: An in vitro study. Dental Hypotheses. 2012; 3(3): 99-105.

Fani M, Kohanteb J. Inhibitory activity of Aloe vera gel on some clinically isolated cariogenic and periodontopathic bacteria. Journal of Oral Science.2012; 54(1):15-21.

Gao X, Seneviratne CJ, Lo ECM, Chu CH, Samaranayake LP. Novel and conventional assays in determining abundance of Streptococcus mutans in saliva. IJPD. 2011: 1-6.




DOI: http://dx.doi.org/10.30659/odj.5.1.54-59

Refbacks

  • There are currently no refbacks.


Lisensi Creative Commons
This work is licensed under a Lisensi Creative Commons Atribusi-BerbagiSerupa 4.0 Internasional.
Contact us: Odonto Dental Journal: Jl. Raya Kaligawe Km.4, PO BOX 1054/SM Semarang, Central Java, Indonesia, 50112. Email: odontodentaljournal@unissula.ac.id