Cd8+ expression in oral potentially malignant disorders associated with risk factors in selected population of east Indonesia

Irene Tania Bijaya, Firstine Kelsi Hartanto, Rahmi Amtha, Indrayadi Gunardi

Abstract


Background: Oral potentially malignant disorder (OPMD) is a lesion with a high potential to turn into oral squamous cell carcinoma (OSCC). The discovery of OPMD lesions precedes most cases of OSCC. Sensitive and specific biomarkers can help in the early detection of high-risk patients, one of which is CD8+, part of the cytotoxic T lymphocytes, which have a role in eliminating cancer cells. Several studies use infiltration density and CD8+ T cells' activity as biomarkers in malignancy. This study aims to observe the expression of CD8+ in OPMD lesions and correlate with risk factors
Method: Twelve paraffin blocks samples consist of OPMD lesions. One oral lichen planus (OLP) as the positive control. Samples were stained using HE to observe the degree of dysplasia and immunohistochemistry to observe the expression of CD8+ T cells. Observation of results using a microscope with 100x magnification to select the field of view and 400x magnification to count the number of positive CD8+ T cells with a cut-off point of 500 cells from a total of 5 fields of view and determined by weak intensity (<500 cells) and strong intensity (≥ 500 cells).
Result: 61,5% of samples showed weak intensity CD8+ T cell, and 38.5% showed strong intensity. There is a significant correlation between risk factors and CD8+ expression (p=0.005).
Conclusion: Expression of CD8+ T cells with strong intensity was found in OPMD lesions with moderate to severe dysplasia. Patients with risk factors showed CD8+ T-cell expression at a weaker intensity than those without risk factors.

Keywords


OPMD; dysplasia,CD8+ T cells; biomarkers; immunohistochemistry

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References


Bergmeier LA. Oral mucosa in health and disease: A concise handbook. Oral Mucosa in Health and Disease: A Concise Handbook. 2018. 105–115 p.

Prestiyanti NMI. The role of salivary biomarker as a diagnostic tool in oral cancer: a literature review. Intisari Sains Medis. 2020;11(1):112.

Wetzel SL, Wollenberg J. Oral Potentially Malignant Disorders. Vol. 64, Dental Clinics of North America. 2020. 25–37 p.

Kirita T, Tokyo KOOCC. Oral cancer. Kirita T, editor. Vol. 19, Springer Japan. Japan: Springer Berlin Heidelberg; 2015. 83–95 p.

Farah CS, Woo S Bin, Zain RB, Sklavounou A, McCullough MJ, Lingen M. Oral cancer and oral potentially malignant disorders. Int J Corros. 2014;2014.

Mello FW, Miguel AFP, Dutra KL, Porporatti AL, Warnakulasuriya S, Guerra ENS, et al. Prevalence of oral potentially malignant disorders: A systematic review and meta-analysis. Vol. 47, Journal of Oral Pathology and Medicine. 2018. 633–640 p.

Sarode SC, Sarode GS, Tupkari J V. Oral potentially malignant disorders: A proposal for terminology and definition with review of literature. J Oral Maxillofac Pathol. 2014;18(5):77–80.

Porter S, Gueiros LA, Leão JC, Fedele S. Risk factors and etiopathogenesis of potentially premalignant oral epithelial lesions. Oral Surg Oral Med Oral Pathol Oral Radiol. 2018;125(6):603–11. Available from: https://doi.org/10.1016/j.oooo.2018.03.008

Hartanto FK, Karen-Ng LP, Vincent-Chong VK, Ismail SM, Wan Mustafa WM, Abraham MT, et al. KRT13, FAIM2 and CYP2W1 mRNA expression in oral squamous cell carcinoma patients with risk habits. Asian Pacific J Cancer Prev. 2015;16(3):953–8.

Ojeda D, Huber MA, Kerr AR. Oral Potentially Malignant Disorders and Oral Cavity Cancer. Dermatol Clin. 2020;38(4):507–21. Available from: https://doi.org/10.1016/j.det.2020.05.011

Maymone MBC, Greer RO, Kesecker J, Sahitya PC, Burdine LK, Cheng AD, et al. Premalignant and malignant oral mucosal lesions: Clinical and pathological findings. J Am Acad Dermatol [Internet]. 2019;81(1):59–71. Available from: https://doi.org/10.1016/j.jaad.2018.09.060

Vail M, Robinson S, Condon H. Recognition of oral potentially malignant disorders and transformation to oral cancer. JAAPA. 2020;33(11):14–8.

Halim E, Dwiaidina Y, Hadriyanto W, Santosa P, Mulyawati E, Kristanti Y. The role of magnetic resonance imaging in the diagnosis and staging of oral squamous cell carcinoma: case reports. ODONTO Dent J. 2022;9(1):115–30.

Santosh A, Jones T, Harvey J. A review on oral cancer biomarkers: Understanding the past and learning from the present. J Cancer Res Ther. 2016;12(2):486–92.

Mishra R. Biomarkers of oral premalignant epithelial lesions for clinical application. Oral Oncol. 2012;48(7):578–84. Available from: http://dx.doi.org/10.1016/j.oraloncology.2012.01.017

Sukswai N, Khoury JD. Immunohistochemistry Innovations for Diagnosis and Tissue-Based Biomarker Detection. Curr Hematol Malig Rep. 2019;14(5):368–75.

Yang Z, Yan G, Zheng L, Gu W, Liu F, Chen W, et al. YKT6, as a potential predictor of prognosis and immunotherapy response for oral squamous cell carcinoma, is related to cell invasion, metastasis, and CD8+ T cell infiltration. Oncoimmunology. 2021;10(1):1–18. Available from: https://doi.org/10.1080/2162402X.2021.1938890

Chaves ALF, Silva AG, Maia FM, Lopes GFM, de Paulo LFB, Muniz LV, et al. Reduced CD8 + T cells infiltration can be associated to a malignant transformation in potentially malignant oral epithelial lesions. Clin Oral Investig. 2019;23(4):1913–9.

Zhou C, Wu Y, Jiang L, Li Z, Diao P, Wang D, et al. Density and location of CD3+ and CD8+ tumor-infiltrating lymphocytes correlate with prognosis of oral squamous cell carcinoma. J Oral Pathol Med. 2018;47(4):359–67.

Shimizu S, Hiratsuka H, Koike K, Tsuchihashi K, Sonoda T, Ogi K, et al. Tumor-infiltrating CD8+ T-cell density is an independent prognostic marker for oral squamous cell carcinoma. Cancer Med. 2019;8(1):80–93.

Enomoto A, Sato E, Yasuda T, Isomura T, Nagao T, Chikazu D. Intraepithelial CD8+ lymphocytes as a predictive diagnostic biomarker for the remission of oral lichen planus. Hum Pathol [Internet]. 2018;74:43–53. Available from: https://doi.org/10.1016/j.humpath.2017.12.008

Surendran S, Aboelkheir U, Tu AA, Magner WJ, Sigurdson SL, Merzianu M, et al. T-Cell Infiltration and Immune Checkpoint Expression Increase in Oral Cavity Premalignant and Malignant Disorders. Biomedicines. 2022;10(8).

Speight PM, Khurram SA, Kujan O. Oral potentially malignant disorders: risk of progression to malignancy. Oral Surg Oral Med Oral Pathol Oral Radiol. 2018;125(6):612–27. Available from: https://doi.org/10.1016/j.oooo.2017.12.011

Amtha R, Gunardi I, Astoeti TE, Roeslan MO. Characteristic of oral medicine patient using teledentistry during covid-19 pandemic. ODONTO Dent J. 2021;8(1):25. Available from: https://jurnal.unissula.ac.id/index.php/odj/article/view/15273

Woo S Bin. Oral Epithelial Dysplasia and Premalignancy. Head Neck Pathol [Internet]. 2019;13(3):423–39. Available from: http://dx.doi.org/10.1007/s12105-019-01020-6

Öhman J, Magnusson B, Telemo E, Jontell M, Hasséus B. Langerhans Cells and T Cells Sense Cell Dysplasia in Oral Leukoplakias and Oral Squamous Cell Carcinomas - Evidence for Immunosurveillance. Scand J Immunol. 2012;76(1):39–48.

Stasikowska-Kanicka O, Wągrowska-Danilewicz M, Danilewicz M. CD8+ and CD163+ infiltrating cells and PD-L1 immunoexpression in oral leukoplakia and oral carcinoma. Apmis. 2018;126(9):732–8.

Kumar M, Nanavati R, Modi T, Dobariya C. Oral cancer: Etiology and risk factors: A review. J Cancer Res Ther. 2016;12(2):458–63.

Sari RK, Hendarti HT, Soebadi B, Hadi P, Radithia D, P AE. Correlation of ki67 expression to degree of dysplasia in lingual epithelial dysplasia in rattus norvegicus exposured by cigarette smoke (Hubungan Ekspresi Ki67 Terhadap Derajat Displasia Pada Epitel Mukosa Lidah Rattus norvegicus yang Dipapar Asap Rokok). ODONTO Dent J. 2019;6(2):76.

Zhong L, Liu Y, Wang K, He Z, Gong Z, Zhao Z, et al. Biomarkers: paving stones on the road towards the personalized precision medicine for oral squamous cell carcinoma. BMC Cancer. 2018;18(1):1–20.




DOI: http://dx.doi.org/10.30659/odj.10.1.52-60

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