Adisty Restu Poetri, Kwartarini Murdiastuti, Sudibyo sudibyo


Background: Gingival recession is a condition that occurs when the gingival margin is more apically than the cemento-enamel junction and causes the tooth root to open, so a root closure procedure is required. Platelet rich plasma (PRP) contains growth factors, including Fibroblast growth factor which plays a role in the process of angiogenesis and cell proliferation. The activation time is known to affect platelet count and FGF expression. The purpose of this study was to determine the effect of the activation time of platelet rich plasma and collagen on the expression of fibroblast growth factor in the gingival crevicular fluid
Method: The research sample was gingival crevicular fluid which was taken using paper point #30 for 30 seconds as many as 18 samples. Samples were taken before surgery, day 5 and day 7 after surgery. The samples were divided into 2 groups based on the activation time, 1 hour and 24 hours PRP-collagen. Changes in FGF levels were examined using ELISA. Repeated Meassure ANOVA test was conducted to compare the differences in each time observation and post hoc was performed to compare the differences between groups for each time observation.
Result: There was no significant difference in FGF levels in the gingival crevicular fluid at each time and between groups.
Conclusion: There was no effect of the activation time of platelet rich plasma and collagen on the levels of fibroblast growth factor in gingival crevicular fluid


Platelet rich plasma; Collagen; Fibroblast growth factor; Gingival Crevicular Fluid; Gingival recession


Lafzi, A., Nader, A., Amir, E., Assessment of the Etiologic Factors of Gingival Recession in a Group of Patients in Northwest Iran. Journal of Dental Research, Dental Clinics, Dental Prospects, 2009, 3 (3): 90.

Avinash, K., Selvan, T., Coronally Advanced Flap In The Treatment Of Recession Coverage, Int J Dent Case Reports, 2014, 4(1): 1-10.

Naik, AR., Ramesh, AV., Dwarkanath, CD., Use of Autologous Platelet Rich Plasma to Treat Gingival Recession in esthetic Periodontal Surgery, J Indian Soc Periodontol,2013, 17(3): 345-53.

Wolf, HF., Rateitschak, KH., Hassell, TM., Color Atlas of Dental Medicine Periodontology, 3rd revised and expanded edition, 2005, Thieme, New York.

Petrungaro, PS., Using Platelet Rich Plasma to Accelerate Soft Tissue Maturation in Esthetic Periodontal Surgery, Compendium, 2001, 22(9): 1-8.

Albanese, A., Licata, ME., Polizzi, B., Campisi, G., Platelet-rich plasma (PRP) in dental and oral surgery: from the wound healing to bone regeneration, Albanese et al. Immunity & Ageing, 2013, 10: 23.

Park, Hong-Bun, Yang, Jeong-Hee, Chung, Kwang-Hoe, Characterization of the Cytokine Profile of Platelet Rich Plasma (PRP) and PRP-inducted Cell Proliferation and Migrations: Upregulation of Matrix Metalloproteinase-1 amd -9 in HaCaT Cells, The Korean Journal of Hematology, 2011, 46 (4): 265-72.

Rodrigues, SV., Acharya, AB., Thakur, SL., Platelet-Rich Plasma A Review, The New York State Dental Journal, 2012, 2012: 26-30.

Fufa, D., Shealy, B., Jacobson, M., Kevy, S., Murray, MM., Activation of Platelet-Rich Plasma Using Soluble Type I Collagen, J Oral Maxillofac Surg, 2008, 66(4): 684-90.

Harrison, S., Vavken, P., Kevy, S., Jacobson, M., Zurakowski, D., Murray, MM., Platelet Activation by Collagen Provides Sustained Release of Anabolic Cytokines, Am J Sports Med, 2011, 39(4): 729-34.

Cho, HS., Song, IH., Park, SY., Sung, MC., Ahn, MW., Song, KE., Individual Variation in Growth Factor Concentrations in Platelet Rich Plasma and Its Influence on Human Mesenchymal Stem Cells, Korean J Lab Med, 2011, 31: 212-18.

Sood, S., Gupta, S., Mahendra, A., Gene Therapy With Growth Factors for Periodontal Tissue Engineering – A Review, Med Oral Patol Oral Cir Bucal, 2012, 17(2): e301-310.

Nath, SG., Raveendran, R., An Insight Into The Possibilites of Fibroblast Growth Factor in Periodontal Regeneration, Journal of Indian Society of Periodontology, 2014, 18(3): 289-92.

Tanimoto, K., Ohkuma, S., Tanne, Y., Kunimatsu, R., Hirose, N., Mitsuyoshi, T., Yoshimi, Y., Su, S., Tanne, K., , Effects of bFGF on the Modulation of Apoptosis in Gingival Fibroblasts with Different Host Ages, International Journal Dentistry, 2013, 2013: 1-7.

Kaval, B., Renaud, DE., Scott, DA., Buduneli, N., The Role of Smoking and Gingival Crevicular Fluid Markers on Coronally Advanced Flap Outcomes, J Periodontol, 2014, 85(3): 395-405.

Ozdemir., B., Ozmeric, N., Elgun, S. dan Baris, E., Smoking and gingivitis: focus on inducible nitric oxide synthase, nitric oxide and basic fibroblast growth factor, J Periodont Res, 2015.

Vermolen, FJ., Van Baaren, E. dan Adam, JA., A Simplified Model for Growth Factor Induced Healing of Wounds, Mathematical and Computer Modelling, 2006, 44: 887-98.

Kaigler, D., Cirelli, JA., Giannobile, WV., Growth factor delivery for oral and periodontal tissue engineering. Expert Opin Drug Deliv, 2006, 3: 647-62.

DOI: http://dx.doi.org/10.30659/odj.9.0.162-169


  • There are currently no refbacks.

Lisensi Creative Commons
This work is licensed under a Lisensi Creative Commons Atribusi-BerbagiSerupa 4.0 Internasional.
Contact us: ODONTO Dental Journal: Jl. Raya Kaligawe Km.4, PO BOX 1054/SM Semarang, Central Java, Indonesia, 50112. Email: odontodentaljournal@unissula.ac.id