Muhamat Muhtar S Abdurrohman, Rama Putranto, Budi Suhartono, Dhiya almanda Fa'adiyah



Background: Aloe vera (AV) contains a variety of characteristic antibiotic and anti-inflammatory components that have potential advantages for periodontal disease therapy. Diagnostic and prognostic markers of periodontal disease through the activity of alkaline phosphatase (ALP) in gingival crevicular fluid. This study was conducted to evaluate the effect of giving AV gel on alkaline phosphatase levels in rats with bacteria-induced periodontitis
Method: Thirty-six male Sprague dawley were healthy and active, weighing 170-200 grams, aged 2 months were distinguished into two groups: the control group of periodontitis given therapy Gel Metronidazole plus 25% and AV gel 99.03% as a treatment group. Aloe vera gel is administered topically in gingival sulcus at a dose of 300 mg/kg of rat weight. Samples were taken from gingival sulcus liquid on days 1, 2 and 3 using periopaper paper. Alkaline phosphatase levels are measured using a UV/Vis Spectrophotometer with a wavelength of 405 nm. Variant analysis is used to compare ALP levels in different groups
Result: There was significant difference in alkaline phosphatase levels between the control group and the treatment group (p>0.05). In Sprague dawley rats with periodontitis, there was a significant decrease in ALP levels on days 1, 2 and 3 given Metronidazole gel plus 25% therapy and AV gel 99.03%. But the treatment group showed lower ALP levels than the control group
Conclusion: This study revealed that the use of AV gel locally has an effect and can be as an adjunct therapy for periodontitis


Alkaline phosphatase; Aloe vera; Periodontitis


Soud P, Gupta HL, Kumar P, Sethi S, Yadav N. Estimation and Comparison of Levels of Alkaline Phosphatase ( ALP ), Acid Phosphatase ( ACP ), Calcium ( Ca ) and Potassium ( K ) in Serum of Subjects with and Without Periodontal Disease ( PD ). Int J Appl Dent Sci. 2015;1(4):90-93.

Amalina R, Mahdalena M, Aditya G. Differences in the salivary inorganic ions levels and salivary flow rate of the periodontitis and non-periodontitis patients. Padjadjaran J Dent. 2020;32(2):160-165. doi:10.24198/pjd.vol30no3.21201.

Yendluri Durga Bai, R A. Estimation of GCF Alkaline Phosphatase Levels in Health and Periodontal Disease – A Clinico Biochemical Study. Int J Contemp Med Res [IJCMR]. 2018;5(7):5-8. doi:10.21276/ijcmr.2018.5.7.7.

Malhotra R, Grover V, Kapoor A, Kapur R. Alkaline phosphatase as a periodontal disease marker. Indian J Dent Res. 2010;21(4):531-536. doi:10.4103/0970-9290.74209.

Maschinen B, Investition A, Beschaffungen G, Ersatzbeschaffungen B, Mittelherkunft S. Relationship between bone-type alkaline phosphatase levels in gingival crevicular fluid and clinical parameters during supportive periodontal therapy. J Jpn Soc Periodontal. 2018.

Gupta S, Chhina S, Arora SA. A systematic review of biomarkers of gingival crevicular fluid : Their predictive role in diagnosis of periodontal disease status Journal of Oral Biology and Craniofacial Research A systematic review of biomarkers of gingival crevicular fl uid : Their pre. J Oral Biol Craniofacial Res. 2019;8(2):98-104. doi:10.1016/j.jobcr.2018.02.002.

Miani PK, Do Nascimento C, Sato S, Filho A V., Da Fonseca MJV, Pedrazzi V. In vivo evaluation of a metronidazole-containing gel for the adjuvant treatment of chronic periodontitis: Preliminary results. Eur J Clin Microbiol Infect Dis. 2012;31(7):1611-1618. doi:10.1007/s10096-011-1484-7.

Ashouri Moghaddam A, Radafshar G, Jahandideh Y, Kakaei N. Clinical Evaluation of Effects of Local Application of Aloe vera Gel as an Adjunct to Scaling and Root Planning in Patients with Chronic Periodontitis. J Dent (Shiraz, Iran). 2017;18(3):165-172.

Singh P, Jain M, Saxena V, Sharva V, Boddun M, Jain N. Evaluation of local – delivery system containing 80% aloe vera gel used as an adjunct to scaling and root planning in chronic periodontitis: A clinical study. Dent Oral Maxillofac Res. 2019;5(4):1-5. doi:10.15761/domr.1000309.

Heng HC, Zulfakar MH, Ng PY. Pharmaceutical applications of Aloe vera. Indones J Pharm. 2018;29(3):101-116. doi:10.14499/indonesianjpharm29iss3pp101.

Virdi H, Jain S, Sharma S. Effect of locally delivered aloe vera gel as an adjunct to scaling and root planing in the treatment of chronic periodontitis: A clinical study. Indian J Oral Sci. 2012;3(2):84. doi:10.4103/0976-6944.106460.

Bhat G, Kudva P, Dodwad V. Aloe vera: Nature’s soothing healer to periodontal disease. J Indian Soc Periodontol. 2011;15(3):205-209. doi:10.4103/0972-124X.85661.

Hermanto NR, Syaify A, Sudibyo. Pengaruh Aplikasi Gel Aloe Vera Sebagai Bahan Tambahan Scaling Dan Root Planing Terhadap Penyembuhan Jaringan Periodontal Pada Perawatan Periodontitis Kronis. J Kedokt Gigi. 2015;6(3):307-314.

Shetty S V., Patil AK, Ganeshkar S V. Assessment of acid phosphatase and alkaline phosphatase in gingival crevicular fluid in growing and adult orthodontic patients: An in vivo study. J Indian Orthod Soc. 2015;49(1):10-14. doi:10.4103/0301-5742.158627.

Sunao Uehera H ito et al. Relationship between bone-type alkaline phosphatase levels in gingival crevicular fluid and clinical parameters during supportive periodontal therapy. 2018:26-34.

Hajishengallis G. Immunomicrobial pathogenesis of periodontitis: Keystones, pathobionts, and host response. Trends Immunol. 2014;35(1):3-11. doi:10.1016/

Serio FG, Duncan TB. The Pathogenesis and Treatment of Periodontal Disease. 2015.

Deepika V, Vishnu Priya V, Bedre A, Harsha L. Salivary AST, ALP and CK levels in patients with periodontitis. J Pharm Sci Res. 2015;7(6):341-343



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