EFFECT OF HYDROGEL PROVISION OF SHED AND PRP COMBINANTION ON OSTEOCLAST DECREASING IN THE ALVEOLAR BONE OF MALE WISTAR RAT (IN VIVO)

Rahmat Hidayat, Muhamat Muhtar, Mualif Dayu Pangestu

Abstract


ABSTRACT


Background: The process of orthodontic bone resorption and remodelling occur simultaneously. Areas under continuous stress experience nutritional deficiencies and hypoxia, which results in cell death and cause alveolar bone resorption. The increase in osteoclasts is associated with the inflammatory process because it is accompanied by an increase in inflammatory mediators. The combination of SHED and PRP can induce regeneration of the alveolar bone of teeth and can inhibit the bone resorption process thereby suppressing the inflammatory process.
Method: This research method was experimental post-test only control group design, with a total sample of 32 individuals treated with PRP gel, SHED gel, a combination of PRP and SHED, and povidone iodine with a duration of 14 days. Wedge application to the interdental teeth to produce the effect of orthodontic movement. Analysis of the one way ANOVA hypothesis test found that there was a significant difference in the reduction of osteoclasts in each experimental group (P <0.05).
Result: It can be concluded that the mean osteoclast reduction in the PRP group was 359±107.3, SHED 392±170.5, the combination of PRP and SHED 480±151.3, povidone iodine 480±151.3.
Conclusion: There is an effect of the combination of SHED and PRP on the reduction of osteoclasts in the alveolar bone of male Wistar rat.


Keywords


Osteoclast ; Stem cell human exfoliated decidious teeth (SHED); Platelet rich plasma (PRP)

References


Li Y, Chan YW. ScienceDirect Orthodontic tooth movement : The biology and clinical implications. 2016;5(6):653-9.https://doi.org/10.1016/j.kjms.2018.01.007

Younis, L. T. The Role of Components and Molecules of Periodontal Ligament in Orthodontic Tooth Movement. 2019;2(2), 1–12. https://doi.org/10.5281/zenodo.3555157

Feller, L., Khammissa, R. A. G., Schechter, I., Thomadakis, G., Fourie, J., & Lemmer, J. Biological Events in Periodontal Ligament and Alveolar Bone Associated with Application of Orthodontic Forces; 2015.

Bartold, P. Periodontal Regeneration - Fact of Fiction? Journal of the International Academy of Periodontology.2015;(3): 37–49

Figueiredo;, M. F. M. F. L. Non-Surgical Periodontal Therapy: Mechanical Debridement, Anti Microbial Agent, and other modalities. Journal of the International Academy of Periodontology. 2015;2(1):21–30

Lang, N. P. Non-Surgical Periodontal Therapy: Mechanical Debridement, Anti Microbial Agent, and other modalities. Bangkok: International Academy of Periodontology;2015.

Wang, L. H. Y. L. S. Stem cell- ­ based tooth and periodontal regeneration. 2018; 4(1):696–705. https://doi.org/10.1111/odi.12703

Lee, Y. H. H. J. C. Y. S. Y. Dental Stem Cell and Tooth Banking for Regenerative Medicine. J Exp Clin Med. 2010;1(2):111–117.

Kaur, P. Platelet Rich Plasma: A Novel Bioengineering Concept. Trends Biomater.2011;25 (2): 86–90

Sobhana, K. T. G. C. R. Clinical Evaluation of Use of Platelet Rich Plasma in Bone Healing. 2015;14(1):67–80.

Mokhtar, M. F. A. K. T. P. K. DPSCs and SHED in Tissue Engineering and Regenerative Medicine. The Open Stem Cell Journal.2013; 4(3): 1–6.

Castillo, T. N.Comparison of Growth Factor and Platelet Concentration From Commercial Platelet-Rich Plasma Separation Systems. The American Journal of Sports Medicine. SAGE Publications Inc STM.2010; 39(2): 266–271. doi: 10.1177/0363546510387517

Raymond C Rowe, P. J. S. and M. E. Q. Handbook of Pharmaceutical Excipients, Revue des Nouvelles Technologies de l’Information; 2015.

Bigliardi, P. L. et al. Povidone iodine in wound healing: A review of current concepts and practices. International journal of surgery (London, England). England.2017;44(2):260–268. doi: 10.1016/j.ijsu.2017.06.073

Interactions, O. HHS Public Access. 2018; 59(2): 99–107. https://doi.org/10.1080/03008207.2017.1290085.Osteoblast-Osteoclast

Ono, T.RANKL biology : bone metabolism, the immune system, and beyond’. Inflammation and Regeneration.2020; 3 (1):1–16

Prahasanti, C. Combined Hydroxyapatite Scaffold and Stem Cell from Human Exfoliated Deciduous Teeth Modulating Alveolar Bone Regeneration via Regulating Receptor Activator of Nuclear Factor-Κb and Osteoprotegerin System;2019.

Mihaylova, Z. Platelet Derived Products and Periodontal Ligament Stem Cells. Archives of Stem Cell Research.2017; 7(2):34-45

Jayaraman, P.Stem cells conditioned medium : a new approach to skin wound healing management’.2019; 9(9):1–7. doi: 10.1002/cbin.10138

Qian, Y. Platelet-rich plasma derived growth factors contribute to stem cell differentiation in musculoskeletal regeneration’, Frontiers in Chemistry.2017; 5(10): 1–8. doi: 10.3389/fchem.2017.00089.




DOI: http://dx.doi.org/10.30659/odj.8.2.113-118

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