THE EFFECTIVITY OF SCAFFOLD FROM ANADARA GRANOSA SHELL-STICHOPUS HERMANNI ON BLOOD VESSEL COUNTS AFTER TOOTH EXTRACTION

Rima Parwati Sari, Chaterina Dyah Nanik Kusumawardani, Retno Pudji Rahayu, Sri Agoes Soedjarwo, Dian Widya Damaiyanti

Abstract


ABSTRACT

Background: Alveolar bone resorption can occur after tooth extraction. One source of bone scaffold material is hydroxyapatite and tricalcium phosphate (HA-TCP). The shell of Anadara granosa can be synthesized to be HA-TCP. Hyaluronic acid, which is widely contained in Stichopus hermanni, can stimulate endothelial progenitor cells for the healing process.
Purpose: This research aims to prove the Effectivity of scaffold from Anadara granosa shell-Stichopus hermanni on blood vessel counts after tooth extraction.
Methods: The sample in this study was male Wistar rats divided into four study groups randomly. Each group consists of 6 rats. Extraction was carried out on the mandibular first incisor, then was given gelatin as a placebo in the control group, treatment groups given scaffold from Anadara granosa shells (AG), and scaffold combination from Anadara granosa shells-Stichopus hermanni with concentrations of 0.4%-0.8%. (AGSH1-AGSH2). Development of HA-TCP synthesized from Anadara granosa combined with Stichopus hermanni extract for biomedical scaffolds using the freeze-dried method. Observations were made three days after tooth extraction. Data analysis has used one-way ANOVA followed by Tukey HSD (p<0.05).
Results: The results of the ANOVA test followed by the HSD-Tukey test showed a significant difference between C (7.33±1.51) and AGSH1 (11.50±1.38), AGSH2 (21.17±1.94), AG (9.33±1.63) and AGSH1 with AGSH2, but between AG and, AGSH1 no significant differences.
Conclusions: Combination scaffold from Anadara granosa shell and Stichopus hermanni 0.8% effectively increased blood vessel counts after tooth extraction.


Keywords


Anadara granosa shell; Stichopus hermanni; Tooth extraction, Blood vessel

Full Text:

PDF

References


Badan Penelitian Dan Pengembangan Kesehatan. Riset Kesehatan Dasar 2013. Badan Penelitian Dan Pengembangan Kesehatan, Kementerian Kesehatan RI. 2013. P. 110-119

Van der Weijden F, Dell’Acqua F, Slot DE. Alveolar bone dimensional changes of post-extraction sockets in humans: a systematic review. J Clin Periodontol 2009; 36: 1048–1058.

Velnar T, Bailey T, Smrkolj V. The Wound Healing Process: An Overview of The Celluler and Molecular Mechanisms. The Journal of International Medical Research, 2009, 37(5): 1528-1542.

Mitchell RN, Kumar V, Abbas AK, Fausto N, Aster J. Pocket companion to Robbins and Cotran: Pathologic Basis of Disease. 8th edition. Buku Saku Dasar Patologis Penyakit. Saunders. 2011. p. 57-75

Tonnesen MG, Feng X, Clark RAF. Angiogenesis in wound healing. Department of Dermatology, Health Sciences center T16060, SUNY at Stony Brook, Stony Brook, NY 11794-8165, New York. 2000. p. 40-46

Frisca, Sardjono CT dan Sandra F. Angiogenesis: Patofisiologi dan Aplikasi Klinis. JKM, 2009, 8(2): 174-187.

Da Costa GC, Aras M, Chitre V. Failures in Dental Implants. J Adv Med Dent Scie 2014;2(1):68-81.

Sulaiman SB, Keong TK, Cheng CH, Saim AB,Hj Idrus RB. Tricalcium phosphate/hydroxyapatite (TCP-HA) bone scaffold as potential candidate for the formation of tissue engineered bone. Indian J Med Res. 2013 Jun; 137(6): 1093–1101

Rujitanapanicha, Kumpapanb P, Wanjanoi P. Synthesis Of Hydroxyapatite From Oyster Shell Via Precipitation. Sawittree Energy Procedia 2014; 56 : p. 112 – 117

Mao K, Zhou F, Cui F, Li J, Hou X, Li P, Du M, Liang M, Wang Y. Preparation And Properties Of Α-Calcium Sulphate Hemihydrate And Β-Tricalcium Phosphate Bone Substitute. Biomed Mater Eng 2013. Vol. 23 (3):p.197-210

Mao T, Kamakshi V. Bone Grafts And Bone Substitutes. International Journal of Pharmacy and Pharmaceutical Sciences 2014; Vol 6 suppl 2 : 88-91

Kheirallah M, Almeshaly H. Bone Graft Substitutes for Bone Defect Regeneration. A. Collective Review. International Journal of Dentistry and Oral Science, 2016, 3: 247-257

Gomes JAP, Amankwah R, Powel-Richards A, Dua HS. Sodium Hyaluronate (Hyaluronic Acid) Promotes Migration of Human Corneal Epithelial Cells in Vitro. British Journal of Opthalmology. 2004; 88: 821-825

Sari RP, Wahjuningsih E, Karsini I. Modulation of FGF2 after topical application of Stichopus hermaniigelon traumatic ulcer in Wistar rats. Dent. J. (Maj. Ked. Gigi), September 2014; 47 (3) : 126–129

Sari RP, Sudjarwo SA, Rahayu ARP, Prananingrum W, Revianti S, Kurniawan H, Bachmid AF. The effects of Anadara granosa shell -Stichopus hermanni on bFGF expressions and blood vessel counts in the bone defect healing process of Wistar rats. Dent. J. (Maj. Ked. Gigi) 2017 Desember; 50(4): 194–198

Widiyanta E, Rahmat M, Rahajoe PS. Pengaruh pemberian Asam hialuronat jeli 0,8% terhadap kecepatan penyembuhan luka pasca fraktur simpisis mandibular. Jurnal kedokteran gigi, 2011; II (3): 164-173

Dahiya P, Kamal R. Hyaluronic Acid: A Boon In Periodontal Therapy. N Am J Med Sci 2013; Vol. 5(5):309-15

Earl JS, Wood DJ, Milne SJ. Hydrothermal synthesis of hydroxyapatite. Journal of Physics: Conference Series 2006; 26 : 268–271

Balaz P. Mechanochemistry in Nanoscience and Minerals Engineering. Springer-Verlag Berlin Heidelberg 2008 : 103-132

Tanideh N, Nazhvani SD, Jaberi FM, Mehrabani D, Rezazadeh S, Pakbaz S, Tamadon A, Nikahval B. The Healing Effect of Bioglue in Articular Cartilage Defect of Femoral Candyle in Experimental Rabbit Model. Iranian Red Crescent Medical Journal , 2011 ; 13(9): pp.629-33

Kyzas PA, Stefanou D, Batistatou A, Agnantis NJ. Hypoxia-induced tumor angiogenic pathway in head and neck cancer: an in vivo study. Cancer Lett 2005; 225:297-304.

Yin S, Ellis DE. First-Principles Investigations Of Ti-Substituted Hydroxyapatite Electronic Structure. Physical Chemistry Chemical Physics Issue. 2010.

Xiao X, Wang W, Liu D, Zhang H, Gao P, Geng L, Yuan Y, Lu J, Wang Z. The promotion of angiogenesis induced by three-dimensional porous beta-tricalcium phosphate scaffold with different interconnection sizes via activation of PI3K/Akt pathways. Scientific Reports, 2015; 5 (9409):1-11

Litwiniuk M, Krejner A; Grzela T. Hyaluronic Acid In Inflammation And Tissue Regeneration. Wounds 2016 ;28(3):78-88

Schwertfeger KL, Cowman MK, Telmer PG, Turley EA, McCarthy JB. Hyaluronan, Inflammation, And Breast Cancer Progression. Front Immunol. 2015; 6 (236) : 1-12




DOI: http://dx.doi.org/10.30659/odj.8.2.11-17

Refbacks

  • There are currently no refbacks.


Lisensi Creative Commons
This work is licensed under a Lisensi Creative Commons Atribusi-BerbagiSerupa 4.0 Internasional.
Contact us: ODONTO Dental Journal: Jl. Raya Kaligawe Km.4, PO BOX 1054/SM Semarang, Central Java, Indonesia, 50112. Email: odontodentaljournal@unissula.ac.id