THE EFFECT OF VARIOUS DESENSITIZING AGENT APPLICATION ON IN-OFFICE BLEACHING ON THE NUMBER OF FIBROBLASTS
Abstract
Background: Reactive Oxygen Species (ROS) released from hydrogen peroxide causes inflammation of the pulp. Remineralization action by fluoride-containing desensitizing agent (Casein Phosphopeptides-Amorphous Calcium Fluoride Phosphate/CPP-ACFP) occluding dentinal tubule, thereby minimizing the penetration of hydrogen peroxide into the pulp and affect the number of fibroblasts. The aim of this study was to determine the effect of fluoride-containing desensitizing agent (CPP-ACFP) application: before, after, and before-after in-office bleaching techniques using 40% hydrogen peroxide on the number of fibroblasts.
Method: Twenty-eight maxillary molar teeth were divided into 4 groups, Control Group (bleaching treatment), Group I (CPP-ACFP application before bleaching), Group II (CPP-ACFP application after bleaching), and Group III (CPP-ACFP application before and after bleaching). Rats were sacrificed 5 days after and histological preparations were stained with HE. Fibroblasts counting was performed using a 1000x magnification light microscope.
Result: ANOVA test showed that there were significant mean differences in the number of fibroblasts among the groups. The LSD test showed that there were statistically significant differences in almost all of the compared groups except between the Control Group and Group II.
Conclusion: The number of fibroblasts in the pulp of rats applied CPP-ACFP before and after in-office bleaching was less than applied CPP-ACFP before or after in-office bleaching.
Keywords
Full Text:
PDFReferences
Cartagena, A.F., Parreiras, S.O., Loguercio, A.D., Reis, A., Campanha, N.H. In-office bleaching effects on the pulp flow and tooth sensitivity – case series. Braz. Oral Res. 2015;29(1): 1-6
Silva-Costa, R.S.G., Ribeiro, A.E.L., Assuncao, I.V., Araujo Junior, R.F., Araujo, A.A., Guerra, G.C.B., Borges, B.C.D. In-office tooth bleaching with 38% hydrogen peroxide promotes moderate/severe pulp inflammation and production of ll-1β, TNF-β, GPX, FGF-2 and osteocalcin in rats. JAOS. 2018;26: 1-9
Sato, C., Rodrigues, F.A., Garcia1, D.M., Vidal, C.M.P., Pashley, D.H., Tjäderhane, L., Carrilho, M.R., Nascimento, F.D., Tersariol, I.L.S. Tooth Bleaching Increases Dentinal Protease Activity. J Dent Res. 2013;92(2): 187-192
Alqahtani, M. Q. Tooth Bleaching Procedures and Their Controversial Effects: A Literature Review. The Saudi Dental Journal, 2014;26: 33-46
Maran, B.M., Vochikovski, L., Hortkoff, D.R.A., Stanislawczuk, R., Loguercio, A.D., Reis, A. Tooth sensitivity with a desensitizing – containing at-home bleaching gel – a randomized triple-blind clinical trial. Journal of Dentistry. 2018;72: 64-70
Akbari, M., Nejat, A.H., Farkhondeh, N., Moghadam, S.M., Mohammadipour, H.S. Does at-home bleaching induce systemic oxidative stress in healthy subjects. Australian Dental Journal. 2017;62: 58–64
Abutalib, A.M., Fensa, H. Evaluation of the Human Enamel Surface Morphology after Tooth Bleaching Followed by Remineralizing Agents. IHJSR. 2017;7(3) : 181-189
Pashley, D.H. Dentin Permeability, Dentin Sensitivity, and Treatment Through Tubule Occlusion. JOE. 1986;12(10): 465-474
Arrais, C.A.G., Chan, D.C.N., Giannini, M. Effects Of Desensitizing Agents On Dentinal Tubule Occlusion. J Appl Oral Sci. 2004;12(2): 144-8
Torres, C.R.G., Silva, T.M., Fonseca, B.M., Sales, A.L.L.S., Holleben, P., Di Nicolo, R., Borges, A.B. The Effect of Three Desensitizing Agents on Dentin Hypersensitivity: A Randomized, Split-mouth Clinical Trial. Operative Dentistry. 2014;39(5) : E186-E194
Davari, A.R., Ataei E., Assarzadeh, H. Dentin Hypersensitivity: Etiology, Diagnosis, and Treatment; A Literature Review. J. Dent. Shiraz Univ. Med. Sci. 2013;14(3):136-45
Vanichvatana, S., Auychai, P. Efficacy of Two Calsium Phosphate Pastes on The Reminealization of Artificial Caries: A Randomized Controlled Double- Blind in Situ Study. International Journal of Oral Science. 2013;5, 224-228
Banomyong, D., Kanchanasantikul, P., and Rebecca, HW. Effects of casein phosphopeptide – amorphous calcium phosphate remineralizing paste and 8% arginine desensitizing paste on dentin permeability. Journal of Investigative and Clinical Dentistry. 2013;4: 200–206
Kristanti, Y., Asmara, W., Sunarintyas, S., and Juni H. Efektivitas Desensitizing Agent dengan dan tanpa Fluor pada Metode in Office Bleaching terhadap Kandungan Mineral Gigi (Kajian In Vitro). Maj Ked Gi. 2014; 21(2): 136 – 140
Staquet, M.J., Durand, S.H., Colomb, E., Romeas, A., Vincent, C., Bleicher, F., Lebecque, S., Farges, J.C. Different Roles of Odontoblasts and Fibroblasts in Immunity. J Dent Res. 2008;87(3): 256-261
Dewanti, I.D.A.R., Susilawati, I.D.A., Lestari, P.E., Yani, R.W.E., Wulandari, E., Budirahardjo, R., Setyorini, D., Wibisono, S. Robusta Coffee (Coffeacanephora) Decreasing IL-1α (Interleukin-1α) Expression and Increasing the Number of Fibroblasts in Healing Process in Dental Pulp in Wistar Rats. J. Math. Fund. Sci. 2019;51(1): 68-76
Jeanneau, C., Lundy, F.T., El Karim, I., About, I. Potential Therapeutic Strategy of Targeting Pulp Fibroblasts in Dentin-Pulp Regeneration. JOE. 2017;43(95): S17-S24
Kunarti, S. TGF-β1 As The Trigger Of Pulp Fibroblast Proliferation. Folia Medica Indonesiana. 2008;44(2):67-70
Mescher, A. L. Junquiera’s Basic Histology Text and Atlas. 14th Ed., McGraw Hill Education. New York. 2016;97-100
Nakanishi, T., Takegawa, D., Hirao, K., Takahashi, K., Yumoto, H., Matsuo, T. Roles of dental pulp fibroblasts in the recognition of bacterium-related factors and subsequent development of pulpitis. Japanese Dental Science Review. 2011;47:161-166
Han, N., Zheng, Y., Li, R., Li, X., Zhou, M., Niu, Y., Zhang, Q. β-Catenin Enhances Odontoblastic Differentiation of Dental Pulp Cells through Activation of Runx2. PLOS ONE. 2014;9(2):1-11
Shah, A. Desensitizing Agents: A Review. Medical & Clinical Review. 2017;3(3): 1-3
Oki, A.Q., Bimarahmanda, M.E., Rahardjo, M.B. Increased Number of Fibroblasts and Neovascularization after Tooth Extraction in Wistar Rats with Moderate-Intensity Continuous Exercise. Journal of International Dental and Medical Research. 2018;11(3): 840-845
Natalia, D., 2018, Pengaruh Aplikasi Desensitizing Agent terhadap Jumlah Pembuluh Darah pada Pulpa Gigi pada Perawatan Bleaching Ekstrakoronal dengan Hidrogen Peroksida 40% (Kajian In vivo pada Tikus Wistar), Thesis, Program pendidikan Dokter Gigi Spesialis Fakultas Kedokteran Gigi Universitas Gadjah Mada Yogyakarta
Sabir, A. Respons inflamasi pada pulpa gigi tikus setelah aplikasi ekstrak etanol propolis (EEP). Maj. Ked. Gigi. (Dent. J.). 2005;38(2): 77–83
Cintra, L. T. A., Benetti, F., Ferreira, L. L., Rahal, V., Ervolino, E., Jacinto, R. D., Filho, J. E. G. and Briso, A. L. F. Evaluation of an Experimental Rat Model for Comparative Studies of Bleaching Agents. J Appl Oral Sci. 2016;24 (1): 95-104
Fitria, L., Sarto, M. Profil Hematologi Tikus (Rattus norvegicus Berkenhout, 1769) Galur Wistar Jantan dan Betina Umur 4, 6, dan 8 Minggu. Biogenesis. 2014;2(2): 94-100
Dammaschke, T. Rat molar teeth as a study model for direct pulp capping research in dentistry. Laboratory Animal.2010;44: 1-6
Pribadi, H.A., 2017, Pengaruh Bahan Antioksidan Sodium Askorbat 10% dan 25% terhadap Jumlah Pembuluh Darah Pulpa pada Gigi Tikus Pasca Bleaching Ekstrakoronal dengan Hidrogen Peroksida 40%, Thesis, Program Pendidikan Dokter Gigi Spesialis Fakultas Kedokteran Gigi Universitas Gadjah Mada Yogyakarta
Hargreaves, K.M., Berman, L.H. Cohen’s Pathway of the Pulp, 11th Ed., Elsevier, Missouri, 2016; 537-538, 590-591, 644, e-100
Addis, R., Cruciani. S., Santaniello, S., Bellu, E., Sarais, G., Ventura, C., Maioli, M., Pintore, P. Fibroblast Proliferation and Migration in Wound Healing by Phytochemicals: Evidence for a Novel Synergic Outcome. International Journal of Medical Sciences. 2020;17(8): 1030-1042
Vasconcelos, A.A.M., Gama Cunha, AA.G., Borges, B.C.D., Vitoriano, J.D., et al. Enamel properties after tooth bleaching with hydrogen/carbamide peroxides in association with a CPP-ACP paste. Acta Odontologica Scandinavica. 2012; 70: 337–343
Ghafournia, M., Tehrani, Nekouei, A., et al. In vitro evaluation of dentin tubule occlusion by three bioactive materials: A scanning electron microscopic study. Dental Research Journal. 2018;16(3):166-171
Martini, E.C., Parreiras, S.O., Szez, A.L. Bleaching-induced tooth sensitivity with application of a desensitizing gel before and after in-office bleaching: a triple-blind randomized clinical trial. Clinical Oral Investigations 2018;1-10. https://doi.org/10.1007/s00784-019-02942-9
Andriani, A., Handajani, J., Haniastuti, T. Pulpal inflammation after vital tooth bleaching with 38% hydrogen Peroxide. Dent. J. (Maj. Ked. Gigi). 2012;45(2):89–92
Vaz, M., Lopez, LG., Cardoso, PC., Souza, JB., Batista, AC., Costa, NL., Torres, EM., Estrela, C. Inflammatory Response of Human Dental Pulp to At- Home and In Office Bleaching. J Appl Oral Sci. 2016;24(5): 509-17
Le Bras, M., Clement, M.V., Pervaiz, S., Brenner, C. Reactive oxygen species and the mitochondrial signaling pathway of cell death. Histol Histopathol. 2005;20: 205-220
Li, R. Jia, Z., Trush, M.A. Defining ROS in Biology and Medicine. React Oxyg Species (Apex). 2016;1(1): 9–21
Cintra, L.T.A., Benetti, F., Ferreora, L.L., Gomes-Filhp, J.E., Ervolino, E., Gallinari, M.O., Rahal, V., Briso, A.L.F. Penetration Capacity, Color Alteration and Biological Response of Two In-office Bleaching Protocols. Braz Dent J. 2016;27(2):169-175
Heshmat, H., Ganjkar, M.H., Miri, Y., Fard, M.J.K. The effect of two remineralizing agents and natural saliva on bleached enamel hardness. Dental Research Journal. 2015;13(1):52-27
Ortiz, M.I.G., Alencar, C.M., Paula B.L.F., dkk. Effect of the casein phosphopeptideamorphous calcium phosphate fluoride (CPPACPF) and photobiomodulation (PBM) on dental hypersensitivity: A randomized controlled clinical trial. Plos One. 2019;1-14
Luschaks, V.I., , Free radicals, reactive oxygen species, oxidative stress and its classification. Chemico-Biological Interactions. 2014;224: 164–175
Goldberg, M., Hirata, A. The Dental Pulp: Composition, Properties and Functions. JSM Dent. 2017;5(1): 1079 :1-10
Parreiras, S.O., Favoreto, M.W., Lenz, R.E., dkk. Effect of Prior Application of Desensitizing Agent on the Teeth Submitted to In-Office Bleaching. Braz Dent J. 2020;31(3):236-243
Berkathullah, M., Farook, M.S., Mahmoud, O. The Effectiveness of Remineralizing Agents on Dentinal Permeability. BioMed Research International. 2018;1-13
Pinto, S.C.S., Pochapski, M.T., Wambier, D.S., dkk. In vitro and in vivo analyses of the effects of desensitizing agents on dentin permeability and dentinal tubule occlusion. Journal of Oral Science. 2010;52(1):23-32
DOI: http://dx.doi.org/10.30659/odj.8.1.132-140
Refbacks
- There are currently no refbacks.
This work is licensed under a Lisensi Creative Commons Atribusi-BerbagiSerupa 4.0 Internasional.
Contact us: Odonto Dental Journal: Jl. Raya Kaligawe Km.4, PO BOX 1054/SM Semarang, Central Java, Indonesia, 50112. Email: odontodentaljournal@unissula.ac.id