BAY LEAF (SYZYGIUM POLYANTHUM) EXTRACT EFFECT ON IL-10 EXPRESSION IN ORAL ULCER

Arina Shafia, Rochman Mujayanto, Anggun Feranisa

Abstract


Background: Traumatic ulcers were pathological conditions characterized by loss of epithelial and basal layers resulting from tissue surface excavation caused by trauma. Ulcer healing process consisted of inflammatory, proliferation, and maturation phases. One of the cytokines that play a role in the anti-inflammatory process was IL-10 which worked by balancing the excessive amount of pro-inflammatory cytokines. Bay leaf contained flavonoids which had anti-inflammatory effects. This study’s objective is to determine the effect of bay leaf extract gel on IL-10 expression in the healing process of traumatic ulcers.
Method: This study was an analytical study of true experimental laboratories post-test only, consisting of four groups and a sample of 20 male Wistar rats. Mucous ulcers on rat lip were treated with bay leaf extract gel until the 3rd and 5th days. Rat mucosal tissue would be made histological preparations with immunohistochemical staining and calculated IL-10 expression using the Hotspot method. Data obtained then analyzed using Independent T-Test.
Result: Results explained that there was a significant increase in IL-10 expression in the group treated using 10% bay leaf extract gel than the control group on 3rd and 5th day, which was marked by a significant p <0.05.
Conclusion: The conclusion obtained was 10% bay leaf extract (Syzygium polyanthum) Gel effective in increasing IL-10 expression in the healing of the mucous traumatic ulcer of the mouth of the Wistar rat.


Keywords


Bay Leaf; IL-10; Traumatic Ulcers

References


Sunarjo L, Hendari R, Rimbyastuti H. Manfaat Xanthone Terhadap Kesembuhan Ulkus Rongga Mulut Dilihat dari Jumlah Sel PMN dan Fibroblast. ODONTO Dent J. 2015;2(2):14–21.

Langlais RP, Miller CS, Gehrig JS. Color Atlas of Common Oral Diseases. 5th Edition. Philadelphia: Lippincott Williams & Wilkins; 2016.

Kresno SB. IMUNOLOGI: Diagnosis dan Prosedur Laboratorium. Edisi 5. Jakarta: Badan Penerbit Fakultas Kedokteran Universitas Indonesia; 2013. 114 p.

Silalahi M. Syzygium polyanthum (Wight) Walp. (Botani, Metabolit Sekunder dan Pemanfaatan). J Din Pendidik. 2017;10(1):1–16.

Harismah K, Chusniatun. Pemanfaatan daun salam (Eugenia polyantha) Sebagai Obat Herbal dan Rempah Penyedap Makanan. War LPM. 2016;19(2):110–8.

Utama TIW. Formulasi Sediaan Krim Ekstrak Etanolik Daun Salam (Eugenia polyantha Wight.) Dengan Pengujian Aktivitasnya Sebagai Anti Inflamasi Topikal Pada Tikus. Universitas Sanata Dharma; 2014. 53 p.

Sastroasmoro S, Ismael S. Dasar-dasar Metodologi Penelitian Klinis. 4th ed. Jakarta: Sagung Seto; 2011. 86 p.

Megawati, Yacobus AR, Akhir LO. Formulasi Dan Uji Stabilitas Fisik Sediaan Gel Ekstrak Kulit Buah Rambutan (Nephelium lappaceum L.) Sebagai Obat Sariawan Menggunakan Variasi Konsentrasi Basis Carbopol. [JFS] J Farm Sandi Karsa. 2019;5(1):5–10.

Mujayanto R, Harijanti K, Hernawan I. Topical application of 1% ZnSO4 on oral ulcers increases the number of macrophages in normal or diabetic conditions of wistar rats. Dent J (Maj Ked Gigi). 2016;49(3):133–6.

Kartikandari RM. Analisa Gel Ekstrak Daun Kelor (Moringa oleifera) Terhadap Jumlah Makrofag Pada Penyembuhan Ulkus Traumatikus Mukosa Mulut (In Vivo). Universitas Islam Sultan Agung; 2017. 30 p.

Astari ED. Pengaruh Sari Bonggol Nanas (Ananas comosus (L.) Merr.) terhadap Jumlah Fibroblas pada Proses Penyembuhan Ulkus Traumatikus pada Tikus Galur Wistar. Universitas Islam Sultan Agung; 2016. 47 p.

Setiorini Y, Wresdiyati T. Deteksi Secara Imunohistokimia Imunoglobulin A (IgA) pada Usus Halus Tikus yang Diberi Bakteri Asam Laktat (BAL) dan Enteropatogenik Escherichia coli (EPEC). Institut Pertanian Bogor; 2012. 64 p.

Hartayati MD. Hubungan Positif Ekspresi Cyclooxygenase-2 dengan Microvessel Density pada Undifferentiated Carcinomanasophar YNX di RSUP Sanglah Denpasar. Universitas Udayana Denpasar; 2015. 38 p.

Agustina R, Indrawati DT, Masruhin MA. Aktivitas Ekstrak Daun Salam (Eugenia polyantha) Sebagai Antiinflamasi pada Tikus Putih (Rattus norvegicus). J Trop Pharm Chem. 2015;3(2):120–3.

Rizky MI, Hariandja M. Review : Aktivitas Farmakologis , Senyawa Aktif , dan Mekanisme Kerja Daun Salam (Syzygium polyanthum). In: Prosiding Seminar Nasional & Workshop “Perkembangan Terkini Sains Farmasi & Klinik 5.†Padang: Prodi Farmasi FMIPA, Universitas Lambung Mangkurat, Banjarbaru; 2015. p. 239–44.

López NL, Grijalva EPG, Perez DLA, Heredia JB. Flavonoids as cytokine modulators: A possible therapy for inflammation-related diseases. Int J Mol Sci. 2016;17(6):921.

Ramadhani N, Sumiwi SA. Aktivitas Antiinflamasi Berbagai Tanaman Diduga Berasal Dari Flavonoid. Farmaka. 2015;14(2):111–23.

Saqib U, Sarkar S, Suk K, Mohammad O, Baig MS, Savai R. Phytochemicals as modulators of M1-M2 macrophages in inflammation. Oncotarget. 2018;9(25):17937–50.

Landén NX, Li D, Ståhle M. Transition from Inflammation to Proliferation: A Critical Step During Wound Healing. Cell Mol Life Sci. 2016;73(20):3861–3885.

Duque GA, Descoteaux A. Macrophage Cytokines: Involvement in Immunity and Infectious Diseases. Front Immunol. 2014;5(491):1–12.

Larouche J, Sheoran S, Maruyama K, Martino MM. Immune regulation of skin wound healing: Mechanisms and novel therapeutic targets. Adv Wound Care. 2018;7(7):209–31.




DOI: http://dx.doi.org/10.30659/odj.7.1.53-59

Refbacks

  • There are currently no refbacks.


Lisensi Creative Commons
This work is licensed under a Lisensi Creative Commons Atribusi-BerbagiSerupa 4.0 Internasional.
Contact us: Odonto Dental Journal: Jl. Raya Kaligawe Km.4, PO BOX 1054/SM Semarang, Central Java, Indonesia, 50112. Email: odontodentaljournal@unissula.ac.id