PERBEDAAAN PENURUNAN MASA BIOFILM Streptococcus mutans ANTARA PEMBERIAN FRAKSI N-HEKSANA DAN ETIL ASETAT EKSTRAK ETANOL BAWANG PUTIH SIUNG TUNGGGAL (Allium sativum L.)
DOI:
https://doi.org/10.30659/odj.6.0.21-28Keywords:
Masa biofilm, Streptococcus mutans, bawang putih suing tunggalAbstract
Background: Garlic (Allium sativum L) is one of the most important Allium species consumed worldwide and has been used for decades as a cure for various diseases. The aim of this research was to compare the efficacy of single-clove garlic’s n-hexane and ethyl acetate fractions in inhibiting the growth of Streptococcus mutans biofilm
Method: This was a true experimental research. The biofilm mass was determined by absorbance value at 590 nm wavelength with ELISA reader in a microplate using safranin. Data was analyzed using ANOVA followed by post hoc analysis with Ï value <0.05.
Result: The result showed that average decrease of biofilm mass after 1-30 minutes of ethyl acetate fraction administration was 33.4 ± 8.03 mg/mL, n-hexane 23.6 ± 0.97 mg/mL and chlorhexidine 35.5 ± 0.98 mg/mL. The average decrease of biofilm mass after 30-60 minutes of ethyl acetate fraction administration was 11.94 ± 7.44 mg/mL, n-hexane 43.87 ± 41.6 mg/mL and chlorhexidine 16.35 ± 4.6 mg/ mL. Statistic analysis showed that there was a notable difference in the decrease of Streptococcus mutans biofilm mass (Ï value= 0.00).
Conclusion: The conclusion of this research was that administrating either n-hexane or ethyl acetate fractions of a single-clove garlic has the ability to decrease
S. mutans biofilm mass.
References
Sabir A. Aktivitas Antibakteri flavonoid propolis Trigona sp terhadap bakteri Streptococcus mutans (in vitro). Dent J. 2005;38(3):135-41.
Ngantung RA, Pangemanan DHC, Gunawan PN. Pengaruh tingkat sosial ekonomi orang tua terhadap karies anak di TK Hang Tuah Bitung. Jurnal e-GiGi(eG). 2015;3(2).
Noviani N. Faktor-faktor yang berhubungan dengan status karies gigi (Dmft) santri pesantren Al Ashriyyah Nurul Iman Parung Bogor Tahun 2010. Tesis. 2010:3-4.
Winda SU, Gunawan P, Wicaksono DA. Gambaran karies rampan pada siswa pendidikan anak usia dini di Desa Pineleng II Indah. Jurnal e-GiGi(eG). 2015;3:1.
Widayati N. Faktor yang berhubungan dengan karies gigi pada anak usia 4-6 tahun. Jurnal Berkala Epidemiologi.2(2):196-205.
Fejerskov O, Kidd E. Dental caries; the diesease and its clinical management. Blackwell Publishing Ltd, UK. 2008:166-73.
Sherbiny GME. Control of growth Streptococcus mutans isolated from saliva and dental caries. Curr Microbiol App Sci 2014;3(10):1-10.
David BU, Linda OO, Charles OE. Isolation, characterization and antibiotic susceptibility studies of clinical isolates of Streptocococcus mutans obtained from patients visiting major dental clinics in Nsukka, Nigeria. AJPSP. 2011;2(1):1-15.
Cameron AC, Widmer RP. Dental caries and restorative paediatric dentistry. In Cameron, Editor. Handbook of Pediatric Dentistry. Mosby. 2003:44-5.
Lamont RJ, Burne RA, Lsntz MS, Blance DJL. Oral microbiology and immunology. ASM Press. Washington DC. 2006:233-52.
Pinkham J, Fields HW. Periodontal problem in children and adolescents in Pediatric Dentistry by Pinkham. Saunders Co. 2005.
Hendarwati M. Efek Antibakteri ekstrak daun jeruk purut (Citrus hystrix) terhadap bakteri Streptococcus mutans (In Vitro). Skripsi. 2014:1-3.
Lawal B, Shittu OK, Oibiokpa FI, Mohammed H, Umar SI. Antimicrobial evaluation, acute and sub-acute toxicity studies of Allium sativum. Acute Disease. 2016;5(4):296-301.
Maryati L. Perbandingan daya hambat ekstrak etanol bawang putih (Allium sativum) terhadap Streptococcus mutans ATCC 25175 dalam bentuk planktonik dengan bentuk biofilm secara invitro. Tesis. Universitas Padjadjaran. 2015.
Rayani I. Uji aktivitas antibakteri ekstrak etanol dan beberapa fraksi daun ekor naga (Rhaphidopora Pinnata) terhadap bakteri Streptococcus mutans dan Pseudomonas aeruginosa. Universitas Sumatera Utara Skripsi. 2012:6.
Lestari M. Uji aktivitas antibakteri ekstrak etanol, fraksi n-heksan, fraksi kloroform dan fraksi etilasetat daun jambu mete (Anacardium occidentale L.). Universitas Sumatera Utara Skripsi. 2016:6.
Octareza R. Penapisan Ekstrak daun Zingiberaceae sebagai antibakteri Streptococcus mutans dan pendegradasi biofilm pada gigi. Institut Pertanian Bogor Skripsi. 2016.
Novita W. Uji Aktivitas antibakteri fraksi daun sirih (Piper Betle L) terhadap pertumbuhan bakteri Streptococcus mutans secara in vitro. JMJ. 2016;4(2):140-55.
Nalina T, Sarah P, Nick ZHA. The Crude aqueous extract of pipper betle l and its antibacterial effect towards Streptococcus mutans. Am J Biochem & Biotech. 2003;3(7):105.
Hariana AH. Tumbuhan Obat dan Khasiatnya. Jakarta : Penebar Swadaya. 2007.
Balagopal SRA. Chlorhexidine: The gold standard antiplaque agent. Journal of Pharmacheutical Sciences and Research. 2013;5(12):270-4.
Poureslami HR, Poureslami P, Salari Z, Salari S. Comparison of antibacterial effect of fluoride and chlorhexidine on two cariogenic bacteria: an in vitro study. Journal of Dental Biomaterials. 2014;1(1).
Susanto M. Potensi minyak atsiri beberapa daun Zingiberaceae sebagai antibakteri Streptococcus mutans dan degradator biofilm pada gigi [skripsi]. Bogor (ID): Fakultas Matematika dan ilmu pengetahuan alam jurusan kimia, Institut Pertanian Bogor. 2015.
Ardani M, Pratiwi SUT, Hertiani T. Efek campuran minyak atsiri daun cengkeh dan kulit manis sebagai antiplak gigi. Majalah Farmasi Indonesia. 2010;21(3):191-201.
DePaola LG, Spolarich AE. Safety and efficiency of antimicrobial mouthrinses in clinical practice. J of Dent Hyg. 2007;81(5):1-16.
Sari SPW, Rahmapuspita F, Iriyani N, Pratiwi SUT, Hertiani T. Penelusuran potensi kapulaga, temu putri dan senggugu sebagai penghambat pembentukan biofilm. Jurnal Ilmu Kefarmasian Indonesia. 2014;12(1):17-24.
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