The influence of chlorhexidine gluconate and povidone iodine on the minimum inhibitory cencentration and quantitative of streptococcus mutans
DOI:
https://doi.org/10.30659/odj.12.2.183-189Keywords:
Dental caries, streptococcus mutans, minimum inhibitory concentration, chlorhexidine, povidone iodineAbstract
Background: An imbalance in the normal flora of the oral cavity can cause dental caries. The prevalence of caries in primary teeth in Indonesia which experiences caries is 90.2%, which shows that the prevalence of caries is still quite high. One way to prevent bacterial activity is using mouthwash. Chlorhexidine gluconate and Povidone Iodine are antibacterial agents that are effective in inhibiting bacterial growth, through bacteriostatic and bactericidal actions. This study aims to determine whether there is an inhibitory effect of chlorhexidine gluconate and povidone iodine on the growth of Streptococcus mutans (quantity).
Method: This study used the broth microdilution method by treating Streptococcus mutans using 0.1 % chlorhexidine gluconate and 1% povidone iodine. The effect of inhibitory power was measured using the broth microdilution technique and the total plate count technique in the form of Colony Forming Units (CFU). Statistical calculations use the Shapiro-Wilk normality test, Levene's homogeneity test and Tukey's Post Hoc test to determine differences between treatments.
Result: The results of the research showed that the treatment group in the form of 0.1% chlorhexidine gluconate and 1% povidone iodine had a very significant effect on the growth of bacterial colonies, which was very significant for the growth of Streptococcus mutans bacterial colonies.
Conclusion: Chlorhexidine gluconate 0.1% and povidone iodine 1% were effective in reducing the growth of Streptococcus mutans bacteria.References
Garg N, Garg A, eds. Textbook of Operative Dentistry. Third edition. Jaypee. 2016.
Chen X, Daliri EBM, Kim N, Kim JR, Yoo D, Oh DH. Microbial Etiology and Prevention of Dental Caries: Exploiting Natural Products to Inhibit Cariogenic Biofilms. Pathogens. 2020;9(7):569.
Gao L, Xu T, Huang G, Jiang S, gu Y, Chen F. Oral Microbiome : More and More Importance in Oral Cavity and Whole Body. Protein Cell. 2018;9(5):488-500.
Samaranayake L. essential Microbiology for Dentistry. 4th ed. Churchill Livingstone, Elsevier. 2012.
Delfani S, Mohammadrezaei-Khorramabadi R, Ghamari S, et al. Systematic Review for Phytotherapy in. J Pharm Sci. 2017;9:11.
Lemos J, Palmer S, Zeng L, et.al. The Biology of Streptococcus mutans. Published online 2019:26.
Rao Arathi. Principles and Practice of Pedodontic. 3th ed. Jaypee. 2012.
Brookes ZLS, Bescos R, Belfield LA, Ali K, Roberts A. Current uses of chlorhexidine for management of oral disease: a narrative review. Journal of Dentistry. Published online 2020:10.
Bathla S. Periodontics Revisited. 1st ed. Jaypee Brothers Medical Publishers; 2011.
Lepelletier D, Maillard JY, Pozzetto B, Simon A. Povidone Iodine: Properties, Mechanisms of Action, and Role in Infection Control and Staphylococcus aureus Decolonization. Antimicrob Agents Chemother. 2020;64(9):e00682-20.
Szymanska J, Olejnik E. Active Ingredients of Mouthwashes. Acta Poloniae Pharmaceutica - Drug Research. 2021;77(6):825-832.
Gauba K, Goyal A, Lnu N, Gupta A, Sharda S. Comparing the Effectiveness of Topical Fluoride and Povidone Iodine with Topical Fluoride Alone for the Prevention of Dental Caries among Children: A Systematic Review and Meta-analysis. International Journal of Clinical Pediatric Dentistry. 2021;13(5):559-565.
Murphy PB, Bistas KG, Patel P, Le JK. Clindamycin. StatPearls Publ LLC. Published online 2024.
Gerung WHP, Fatimawali, Antasionasti I. Uji Aktivitas Antibakteri Ekstrak Daun Belimbing Botol (Averrhoa bilimbi L.) Terhadap Pertumbuhan Bakteri Propionibacterium acne Penyebab Jerawat. Pharmacon. 2021;10(4):1087-1093.
James P, Worthington HV, Parnell C, et al. Chlorhexidine mouthrinse as an adjunctive treatment for gingival health. Cochrane Database Syst Rev. 2017;3. doi:10.1002
Chairani S, Aisyah. Perbandingan Pengaruh Ekstrak Bawang Putih Dan Klorheksidin Terhadap Jumlah Candida albicans Pada Pengguna Ortodonti Cekat. J Kesehat Gigi Dan Mulut. 2022;4(2).
Scannapieco FA, Yu J, Raghavendran K, et al. A randomized trial of chlorhexidine gluconate on oral bacterial pathogens in mechanically ventilated patients. Crit Care. Published online July 2019.
Khan MM, Parab SR. Tolerability and Usability of 0.5% PVP-I Gargles and Nasal Drops in 6692 Patients: Observational Study. Am J Otolaryngol. 2021;42(2): 1-4.
Harshitha VR, Jeevitha M. Comparative Evaluation of Wettability of Chlorhexidine, Betadine, and Herbal Mouthwash: An In Vitro Study. World J Dent. 2023;14(7): 634-638.
Lestari D, Ananda LDD, Setiawan H, Prasetyorini T. The Decreased of Germs Number Before and After Gargling with Povidone - Iodine. J Ilmu Dan Teknol Kesehat. 2024;11(2): 268-278.
Downloads
Published
Issue
Section
License
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
This work is licensed under a Creative Commons Attribution 4.0 International License.
