Antibacterial Activity of 96% Ethanol Extract of Green Eggplant (Solanum Melongena L.) Fruit Against Staphylococcus Aureus Atcc-29213 Using Well Diffusion Method

Rose Malinda Andamari Wahyu Utami, Tri Nur Azizah, Ferina Damayanti, Hogi Noni Saputro, Nurul Syazwani, Lucky Restyanti Wahyu Utami

Abstract


Abstract: This study aims to evaluate the antibacterial activity of 96% ethanol extract of green gelatik eggplant (Solanum melongena L.) fruit against Staphylococcus aureus ATCC-29213 bacteria using the well method. The research process includes the preparation of simplicia, ethanol extraction through maceration, qualitative tests with phytochemical screening to identify active compounds, and antibacterial tests using the well method using Staphylococcus aureus ATCC-29213 bacteria as the test material and 96% ethanol extract of green gelatik eggplant (Solanum melongena L.) fruit made in concentrations of 5%, 10% and 15% for comparison. The results showed that the extract contains flavonoids, alkaloids, tannins, and steroids that have the potential as antibacterials. Antibacterial activity test at 5% concentration obtained an average inhibition zone diameter of 0.0033 cm, a concentration of 10% obtained an average inhibition zone diameter of 1.17 cm, and a concentration of 15% obtained an average inhibition zone diameter of 1.58 cm. The data shows an increase in the diameter of the inhibition zone as the concentration increases, although it is still lower than the positive control (amoxicillin). ANOVA analysis showed a significant difference between treatment groups (p = 0.000), indicating a positive relationship between extract concentration and inhibitory power against S. aureus.


Full Text:

PDF

References


Cheung GYC, Bae JS, Otto M. Pathogenicity and virulence of Staphylococcus aureus ABSTRACT. Virulence. 2021;12(1):547-

doi:10.1080/21505594.2021.1878688

Tong SYC, Davis JS, Eichenberger E, Holland TL, Fowler VG. Staphylococcus aureus Infections : Epidemiology ,

Pathophysiology , Clinical Manifestations , and Management. 2015;28(3):603-661. doi:10.1128/CMR.00134-14

Daud, M., Yuliani, F. S., & Wahyuni W. Aktivitas antibakteri ekstrak etanol buah terong ungu (Solanum melongena L.)

terhadap bakteri Staphylococcus aureus dan Escherichia coli. J Farm Galen. 2020;6(1):78–84.

doi:https://doi.org/10.22487/j24428744.2020.v6.i1.13481

Balouiri M, Sadiki M, Ibnsouda SK. Methods for in vitro evaluating antimicrobial activity : A review $. J Pharm Anal.

;6(2):71-79. doi:10.1016/j.jpha.2015.11.005

Qodri UL. Analisis Fitokimia Dan Penentuan Kadar Fenolik Total Pada Ekstrak Etanol Tebu Merah Dan Tebu Hijau (

Saccharum Officinarum L .) Phytochemical Analysis and Determination of Total Phenolic Content in Ethanol Extract of Red

Sugar Cane and Green Sugar Cane ( Saccharum Officinarum L ). 2023;4(2):91-102.

Assay A. Disc Plate Method of Microbiological Antibiotic Assay. 1971;22(4):659-665.

Abdallah, E. M., Khalid, A. S., & Ibrahim N. Antibacterial activity of extracts from Solanum melongena fruits. J Med Plants

Stud. 2016;4(3):196-199.

Othman L, Sleiman A, Abdel-massih RM. Antimicrobial Activity of Polyphenols and Alkaloids in Middle Eastern Plants.

;10(May). doi:10.3389/fmicb.2019.00911

Mekonnen, T. W. et al. Plant-derived alkaloids as antibacterial agents. Frontiers in Pharmacology. Front Pharmacol. 2020;11.

doi:https://doi.org/10.3389/fphar.2020.583868

Zhang, L. et al. (2019). Tannins and their impact on antimicrobial activity. Compr Rev Food Sci Food Safet. 2019;18(3):823-838.

doi:https://doi.org/10.1111/1541-4337.12438

Moglad EH. Antimicrobial potential of medicinal plants against multidrug-resistant bacteria. J King Saud Univ – Sci.

;33(2). doi:https://doi.org/10.1016/j.jksus.2021.101240

Sulianti, I., Fitriana, R., & Nurhayati I. Aktivitas antibakteri ekstrak tumbuhan obat tradisional Indonesia terhadap

Staphylococcus aureus. J Biol Trop. 2022;22(3):453-462. doi:https://doi.org/10.29303/jbt.v22i3.3959


Refbacks

  • There are currently no refbacks.