Increased levels of Follicle Stimulating Hormone (FSH) and sertoli cells count in wistar rats induced with paraquat after green bean sprout extract supplementation
Abstract
Paraquat herbicide disrupts the hormone Follicle Stimulating Hormone (FSH), causes testicular damage and changes in spermatogenesis. This study aims to determine the effect of supplementation of green bean sprout extract (Vigna radiata L.) on FSH levels, Sertoli cell counts in male Wistar rats (Rattus norvegicus) induced by paraquat herbicide. This research uses true experimental with posttest only control group design. A total of 25 rats were divided into 5 groups: P0 was given standard feed, P1 was given paraquat 4 4 mg/g BW, P2 was given green bean sprout extract supplement (GBES) dose 21.6 4 mg/g BW and paraquat 4 mg/g BW P3 was given GBES dose 43.2 4 mg/g BW and paraquat is administered orally for 15 days. Measurement of FSH levels using ELISA, while Sertoli cells were observed using testicular histology preparate. Mann-Whitney test resulted significance of p<0.05, which showed that the administration of GBES influenced FSH levels and the number of Sertoli cells. GBES doses of 21.6 4 mg/g BW, 43.2 4 mg/g BW, and 86.4 4 mg/g BW were shown to increase FSH levels and Sertoli cell counts in paraquat-induced Wistar rats. GBES increased FSH levels and the number of Sertoli cells in paraquat-induced rats.
Downloads
License
© 2022 Author(s). Published by Sains Medika: Jurnal Kedokteran dan Kesehatan. This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
How to Cite
References
Anggraini, D., Sutyarso, S., Kanaedi, M, Busman, H. (2018). Pengaruh pemberian ekstrak etanol jahe merah (Zingiber officinale Roxb var Rubrum) terhadap kuantitas dan kualitas spermatozoa mencit jantan (Mus musculs L.) yang diinduksi paraquat diklorida. Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH), 5(2): 47-54. http://dx.doi.org/10.23960%2Fj_bekh.v5i2.2169
Durairajanayagam, D., Darbandi, S., Sadeghi, M. R., Darbandi, M., Henkel, R., Agarwal, A., & Sengupta, P. (2018). Reactive oxygen species and male reproductive hormones. Reproductive Biology and Endocrinology, 16(1), 1–14. https://doi.org/10.1186/s12958-018-0406-2
Gawarammana, I. B., & Buckley, N. A. (2011). Medical management of paraquat ingestion. British Journal of Clinical Pharmacology, 72(5), 745–757. https://doi.org/10.1111/j.1365-2125.2011.04026.x
Hidayat, E., Susanti, R., & Marianti, A. (2017). Protein profile and mda spermatozoa levels of hyperglycemic mice fed by bean sprouts extract. Indonesian Journal of Pharmacy, 26(4), 192. https://doi.org/10.14499/indonesianjpharm26iss4pp192
Ismael, Z. K., Al-Anbari, L. A., & Mossa, H. A. L. (2017). Relationship of FSH, LH, DHEA and testosterone levels in serum with sperm function parameters in infertile men. Journal of Pharmaceutical Sciences and Research, 9(11), 2056–2061. https://www.jpsr.pharmainfo.in/Documents/Volumes/vol9Issue11/jpsr09111717.pdf
Mahdi, A. A., Rajender, S., & Shukla, K. K. (2012). Apoptosis, spermatogenesis and male infertility. Frontiers in Bioscience-Elite (FBE), 4(2): 746–754; https://doi.org/10.2741/e415
Mulyani, S. S. T. E., Adriani, M., & Wirjatmadi, B. (2016). Effect of mung bean sprouts extract to the morphology and motility spermatozoa in mice exposed monosodium glutamate. International Research Organization for Life & Health Sciences (IROLHS), 2(3): 14–17. https://doi.org/10.17354/ijpphs/2016/34
Novadiyanto, N., Asrullah, M., Arsanti L, L., Helmyati, S., & Farmawati, A. (2019). Effect supplementation of mung bean sprouts (Phaseolus radiatus L.) and vitamin E in rats fed high fat diet. KnE Life Sciences, 4(11), 36-46. https://doi.org/10.18502/kls.v4i11.3850
Oduwole, O. O., Peltoketo, H., & Huhtaniemi, I. T. (2018). Role of Follicle-Stimulating Hormone in spermatogenesis. Frontiers in Endocrinology, 9(763), 1–11. https://doi.org/10.3389/fendo.2018.00763
Parekattil, S. J., & Agarwal, A. (2012). Male infertility: Contemporary clinical approaches, andrology, art & antioxidants. Springer New York, NY1–518. https://doi.org/10.1007/978-1-4614-3335-4
Prasad, S. V., Bb, G., & Bb, N. (2018). Role of oxidative stress and Vitamin C, E on male fertility: Mini role of oxidative stress and Vitamin C, E on Male Fertility: Mini Riview. December 2017. https://www.researchgate.net/publication/329482397_ROLE_OF_OXIDATIVE_STRESS_AND_VITAMIN_C_E_ON_MALE_FERTILITY_MINI_REVIEW
Singh, A., & Agarwal, A. (2011). The role of sperm chromatin integrity and dna damage on male infertility. The Open Reproductive Science Journal, 3: 65-71. http://dx.doi.org/10.2174/1874255601103010065
Wasiu, A. O., & Abdulfatai, A. (2019). Toxic effects of paraquat dichloride leachate on testes and sperm parameters of male wistar rats. International Journal of Anatomy and Research, 7(1.3): 6274–6279. https://doi.org/10.16965/ijar.2018.457
Yusoff, N. A., Budin, S. B., & Taib, I. S. (2017). Pesticide exposures induce male-mediated reproductive toxicity: A review. Journal of Agricultural Science, 9(13), 122-135. https://doi.org/10.5539/jas.v9n13p122
Zhai, L., Xue, Z., Yu, W., Zhou, F., Chang, H., Wang, C., & Kou, X. (2016). Bioactive compounds and antioxidant activity of mung bean (Vigna radiata L.), soybean (Glycine max L.) and black bean (Phaseolus vulgaris L.) during the germination process. Czech Journal of Food Sciences, 34(No. 1), 68–78. https://doi.org/10.17221/434/2015-cjfs
