The Effect of Antlion (Myrmeleon sp.) Extract Towards Histopathology Image of Pancreas in Diabetic Mice

Muhammad Naufal Widyatmaka, Akhmad Ismail


Introduction: Some studies indicated that antlions (Myrmeleon sp.) is traditionally used by many people in Indonesia as a diabetes treatmenter. However. reseach has not been done to find the effect of antlion extract in pancreas, an important organ in diabetes mellitus pathophysiology. Objective: To prove the effect of antlion extract towards histopathological feature of pancreas in diabetic mice.

Methods: In the post test only control group design, 25 Swiss mice were divided into five groups. Normal group (Nor-G), Negative control group (Neg-G), and the other 3 groups were intraperitoneally injected with Streptozotocine 200 mg/kg to induce diabetic state. In Neg-G had no antlion treatment, whereas in At-14, At-28, and At-56 were treated with antlion extract at the dose of 14, 28, and 56 mg/kg BW respectively for 24 days. Pancreas then collected and prepared to be examined under microscope with 400x magnification. The score of pancreatic islet injury was determined by assessing structural damage and inflammatory cells infiltration. Kruskal-Wallis and Mann-Whitney were adopted as statistical analysis.

Results: Mann-Whitney analysis indicated that the score of pancreatic islets injury in At-56 was significant lower compared to that of Neg-G, p<0.05. The score of pancreatic islets injury in At-56 also significant lower compared to that of At-14 and At-28, p<0.05. In contrary, when compared to that of Nor-G, the pancreatic islets injury in At-56 was not signicantly lower, p>0.05.

Conclusion: Antlion extract treatment at the dose of 56 mg/kg BW was capable of reducing pancreatic islet injury in diabetic mice induced by streptozotocine.


Streptozotocine, islet damage, tissue repair

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Widyatmaka and Ismail • pISSN: 2085-1545 • eISSN: 2339-093X Metformin Irrespective of Diabetes Status’, Circulation Research, 119(5), pp. 652–665. doi: 10.1161/CIRCRESAHA.116.308445.

Canetti, L., Werner, H. and Leikin-Frenkel, A. (2014) ‘Linoleic and alpha linolenic acids ameliorate streptozotocin-induced diabetes in mice’, Archives of Physiology and Biochemistry, 120(1), pp. 34–39. doi: 10.3109/13813455.2013.868002.

Furman, B. L. (2015) ‘Streptozotocin-Induced Diabetic Models in Mice and Rats’, Current protocols in pharmacology / editorial board, S.J. Enna (editor-in-chief) ... [et al.], 70(September), pp. 5.47.1-5.47.20. doi: 10.1002/0471141755.ph0547s70.

Griffin, S. J., Leaver, J. K. and Irving, G. J. (2017) ‘Impact of metformin on cardiovascular disease: a meta-analysis of randomised trials among people with type 2 diabetes’, Diabetologia. Diabetologia, 60(9), pp. 1620–1629. doi: 10.1007/s00125-017-4337-9.

Heckman-Stoddard, B. M. et al. (2017) ‘Repurposing metformin for the prevention of cancer and cancer recurrence’, Diabetologia, 60(9), pp. 1639–1647. doi: 10.1007/s00125-017-4372-6.

Ikwegbue, P. C. et al. (2018) ‘Roles of Heat Shock Proteins in Apoptosis , Oxidative Stress , Human Inflammatory Diseases , and Cancer’, pp. 1–18. doi: 10.3390/ph11010002.

International Diabetes Federation (2017) IDF DIABETES ATLAS. 8th editio. Edited by S. Karuranga et al. International Diabetes Federation. Available at:

ISMAIL, T. A., SOLIMAN, M. M. and NASSAN, M. A. (2015) ‘Molecular and immunohistochemical effects of metformin in a rat model of type 2 diabetes mellitus’, Experimental and Therapeutic Medicine, 9(5), pp. 1921–1930. doi: 10.3892/etm.2015.2354.

Jiang, G. J. et al. (2017) ‘Metformin ameliorates insulitis in STZ-induced diabetic mice’, PeerJ, 2017(4), p. e3155. doi: 10.7717/peerj.3155.

Kumar, V., Abbas, A. K. and Aster, J. C. (2014) Robbins & Cotran Pathologic Basis of Disease. 9th edn. Philadelphia, PA: Elsevier Saunders.

Malcicka, M., Visser, B. and Ellers, J. (2018) ‘An Evolutionary Perspective on Linoleic Acid

Synthesis in Animals’, Evolutionary Biology. Springer US, 45(1), pp. 15–26. doi: 10.1007/s11692-017-9436-5.

Maryati, Y. et al. (2019) ‘Antlion (Myrmeleon sp.) Infusion as Antidiabetic in Dexamethasone Induced Mice’, Journal of Physics: Conference Series, 1179(1). doi: 10.1088/1742-6596/1179/1/012177.


Mescher, A. L. (2016) Junqueira’s Basic Histology, Text and Atlas. 14th edn. New York: McGraw-Hill Education. Available at:

Morgan, N. G. and Richardson, S. J. (2018) ‘Fifty years of pancreatic islet pathology in human type 1 diabetes: insights gained and progress made’, Diabetologia. Diabetologia, 61(12), pp. 2499–2506. doi: 10.1007/s00125-018-4731-y.

Muadifah, A., Sulistyarti, H. and Prasetyawan, S. (2017) ‘Liquid Chromatography for Analysis of Metformin in Myrmeleon sp.’, The Journal of Pure and Applied Chemistry Research, 6(3), pp. 196–206. doi: 10.21776/ub.jpacr.2017.006.03.338.

Mujahid, M. Z. et al. (2013) ‘A combination of bitter gourd ethanolic extract with ant lion larvae aqueous extract for a blood glucose-lowering agent’, International Food Research Journal, 20(2), pp. 851–855.

Nahdi, A. M. T. Al, John, A. and Raza, H. (2017) ‘Elucidation of Molecular Mechanisms of Streptozotocin-Induced Oxidative Stress , Apoptosis , and Mitochondrial Dysfunction in Rin-5F Pancreatic β -Cells’, Oxidative Medicine and Cellular Longevity, 2017. doi:

Prihatin, J. et al. (2019) ‘Antihyperglycaemic and tissue-repair effects of Myrmeleon formicarius extract in streptozotocin-induced diabetic mice’, Journal of Taibah University Medical Sciences. Elsevier Ltd, 14(2), pp. 149–155. doi: 10.1016/j.jtumed.2019.01.004.

Rahma, H. H., Sundhani, E. and Nurulita, N. A. (2016) ‘Antidiabetic activity of powder and ethanolic extract of antlion (Myrmeleon sp.) on wistar strain white male rats with glucose preload’Proceeding ICMHS 2016, pp. 14–16.

Rena, G., Hardie, D. G. and Pearson, E. R. (2017) ‘The mechanisms of action of metformin.’, Diabetologia. Diabetologia, 60(9), pp. 1577–1585. doi: 10.1007/s00125-017-4342-z.

Roglic, G. (2016) Global report on diabetes, World Health Organization. Available at:

Rojas, J. et al. (2018) ‘Review Article Pancreatic Beta Cell Death: Novel Potential Mechanisms in Diabetes Therapy’, 2018(Dm).

Saisho, Y. (2015) ‘Metformin and Inflammation: Its Potential Beyond Glucose-lowering Effect’, Endocrine, Metabolic & Immune Disorders-Drug Targets, 15(3), pp. 196–205. doi: 10.2174/1871530315666150316124019.

Volpe, C. M. O. et al. (2018) ‘Cellular death, reactive oxygen species (ROS) and diabetic complications’, Cell Death & Disease, 9(2), p. 119. doi: 10.1038/s41419-017-0135-z.

Walvekar, M. V. et al. (2016) ‘Histological studies on islets of langerhans of pancreas in diabetic mice after curcumin administration’, International Journal of Pharmaceutical and Clinical Research, 8(9), pp. 1314–1318.


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