Effect of White Turmeric Extract (Curcuma zedoaria) Using Zam-zam Solvent Compare with Ethanol Solvent Against Breast Cancer Cell T47D
DOI:
https://doi.org/10.30659/sainsmed.v6i2.601Keywords:
T47D, Cytotoxicity, Curcuma, Zedoaria, Zam-zamAbstract
Introduction: Curcuma zedoaria is one of the herbal plants potentially protects and cures many diseases, particularly as anti-cancer and anti-tumor. Anti-cancer active compounds of it are flavonoid, phenolic, and saponin. Objectives: This study aimed to explore the cytotoxicity of Curcuma zedoria extract (CZE).
Methods: Experimental Quasi with post test non equivalent control group design on T47D cell line at Biology laboratory, Faculty of Medicine, Sultan Agung Islamic University, Semarang. The research was consisted of 2 groups, namely: intervention group with CZE zam-zam solvent and intervention group with CZE ethanol solvent given 10 different dosages each for 1.000 μg/mL; 500 μg/mL; 250 μg/mL; 125 μg/mL; 62.5 μg/mL; 31.25 μg/mL; 15.62 μg/mL; 7.81 μg/mL; 3.90 μg/mL; 1.95 μg/mL. Cytotoxicity test of IC50 using direct counting method and analyzed by probit analysis.
Results: IC50 value of CZE in zam-zam and ethanol solvent were 28.24 μg/ml and 13.71 µg/ml respectively at the same 24 hours incubation period. Chi-square test revealed p value was 0.000 (α = 0.05), meaning that there was significant relationship.
Conclusion: CZE activity using ethanol is highly active while CZE using zam-zam water is moderate and both of them have the toxicity on breast cancer cell. As the result, apoptosis process may occur.
References
Baratawidjaya, K. G. R. I., 2012. Imunologi Dasar Edisi ke 10. Badan Penerbit Fakultas Kedokteran Universitas Indonesia; Jakarta. hal 469-475.
Hiroko D, Teruhiko F, Shino N, Toshihiro K, Kazuo S., 2002. Analisys of Cell growth inhibitory effect of cathechin through MAPK in human breast cancer cell-line T47D. Int J. Oncol.
Kampa, M., Alexaki, I.V., Notas, G., Nifli, P.A., Nistikaki, A., Hatzoglou, A., Bakogeorgou, E., Kouimtzoglou, E., Blekas, G., Boskou, D., Gravanis, A., Castanas, E., 2004. Antiproliferative and Poptotic Effects of Selective Phenolic Acids on T47D Human Breast Cancer Cells: Potential Mechanisme of Action, Breast Cancer Res, 6(2):R63- R74.
Ma’at, Dr. Drs. MS., Apt. Suprapto., 2011. Teknik dasar Kultur Sel. Airlangga University Press. Surabaya.
Manuaba dan Tjakra W, 2010. Panduan Penatalaksanaan Kanker Solid PERBOI 2010. Sagung Seto. Denpasar. hal 17-47.
Peter Boyle and Bernard Levin, 2008. World Cancer Report 2008. Published by the International Agency for Research on Cancer,150 cours Albert Thomas, 69372 Lyon Cedex 08, France. hal 5.
RS Dharmais, 2009. Pasien dan Informasi Kanker. RS.Dharmais Pusat Kanker Nasional. http://www.dharmais.co.id/inde.php/kemoterapi.html (accessed on October 5th, 2014).
Tanamatayarat, P., Limtrakul, P. Chunsakaow, S., Duangrat, C., 2003, Screening of some rubiaceous Plant for Cytotoxic Activity Against Cervix Carcinoma (KB-3-1) Cell line, Thai J,. Pharm, Sci. 27 (3-4): 167-172
Downloads
Published
Issue
Section
License
Journal Author(s) Rights
To publish and disseminate research articles, Sains Medika: Jurnal Kedokteran dan Kesehatan requires non-exclusive publishing rights (transferred from author(s) to publisher). This is determined by a publishing agreement between the Author(s) and Sains Med. J. Kedokt. dan Kesehat. This agreement governs with the transfer or license of the copyright of publishing to Sains Med. J. Kedokt. dan Kesehat, while Authors still retain significant rights to use and share their own published articles. Sains Med. J. Kedokt. dan Kesehat supports the need for authors to share, disseminate and maximize the impact of their research and these rights, in any databases.
As a journal Author, you have rights for a wide variety of uses of your article, including use by your institution or company of employment. These Author rights can be exercised without the need to obtain specific permission. Authors publishing in Sains Med. J. Kedokt. dan Kesehat have wide rights to use their works for teaching and scholarly purposes without needing to seek permission, including:
- Use for classroom teaching by Author or Author's institution and presentation at a meeting or conference and distributing copies to attendees;
- Use for internal training by author's company;
- Distribution to colleagues for their research use;
- Use in a subsequent compilation of the author's works;
- Inclusion in a thesis or dissertation;
- Reuse of portions or extracts from the article in other works (with full acknowledgement of final article);
- Preparation of derivative works (other than commercial purposes) (with full acknowledgement of final article);
- Voluntary posting on open web sites operated by author or author's institution for scholarly purposes,
Authors/Readers/Third Parties can copy and redistribute the material in any medium or format, as well as remix, transform, and build upon the material for any purpose, including commercial purposes, but they must give appropriate credit (the name of the creator and attribution parties (authors detail information), a copyright notice, an open access license notice, a disclaimer notice, and a link to the material), provide a link to the license, and indicate if changes were made (Publisher indicates the modification of the material (if any) and retain an indication of previous modifications using a CrossMark Policy and information about Erratum-Corrigendum notification).
Authors/Readers/Third Parties can read, print and download, redistribute or republish the article (e.g. display in a repository), translate the article, download for text and data mining purposes, reuse portions or extracts from the article in other works, sell or re-use for commercial purposes, remix, transform, or build upon the material, they must distribute their contributions under the same license as the original Creative Commons Attribution (CC BY).
Copyright Transfer Agreement for Publishing (Publishing Right)
The Authors submitting a manuscript do so on the understanding that if accepted for publication, the non-exclusive right for publishing (publishing right) of the article shall be assigned/transferred to the Publisher of Sains Medika: Jurnal Kedokteran dan Kesehatan and Faculty of Medicine Universitas Islam Sultan Agung (FK UNISSULA).
Upon acceptance of an article, authors will be required to complete a 'Copyright Transfer Agreement for Publishing (CTAP)'. An e-mail will be sent to the Corresponding Author confirming receipt of the manuscript together with a 'Copyright Transfer Agreement for Publishing' form by online version of this agreement.
Sains Medika: Jurnal Kedokteran dan Kesehatan and Faculty of Medicine Universitas Islam Sultan Agung (FK UNISSULA), the Editors and the Advisory International Editorial Board make every effort to ensure that incorrect or misleading data, opinions or statements be published in the journal. In any way, the contents of the articles and advertisements published in the Sains Medika: Jurnal Kedokteran dan Kesehatan are sole and exclusive responsibility of their respective authors and advertisers.
Remember, even though we ask for a transfer of copyright for publishing (CTAP), our journal Author(s) retain (or are granted back) significant scholarly rights as mentioned before.
The Copyright Transfer Agreement for Publishing (CTAP) Form can be downloaded here: [Copyright Transfer Agreement for Publishing (CTAP) Form Sains Medika 2022]
The copyright form must be electronically signed and emailed to the Editorial Office in the original form below:
Dr. Suparmi, S.Si, M.Si (ERT) (Editor-in-Chief)
Editorial Office of Sains Medika: Jurnal Kedokteran dan Kesehatan
Gedung Dekanat Lantai 1 Fakultas Kedokteran Universitas Islam Sultan Agung
Jl. Raya Kaligawe KM 4 Semarang 50112, Central Java, Indonesia
Telp/Whatsapp: +62 812-2993-3369
E-mail: sainsmedika@unissula.ac.id
(This policy statements has been updated 19 October 2022)
What is Crossmark?
CrossMark, a multi-publisher initiative from CrossRef, provides a standard way for readers to locate the authoritative version of a document. Sains Medika: Jurnal Kedokteran dan Kesehatan acknowledges the significance of the integrity and completeness of the scholarly record to scholars and librarians, and places the utmost importance on preserving the credibility of its electronic archive. Clicking on the CrossMark icon will inform the reader of a document's current status and may provide additional publication record information.
