Antioxidant activity and phytochemical profiling of peaches, Arabica coffee, and their combination

Authors

  • Dhea Angela Putri Medical Study Program, Faculty of Medicine, YARSI University, Central Jakarta
  • Himmi Marsiati Doctoral Program, Biomedical Sciences, YARSI University, Central Jakarta
Published 2024-12-05
Issue Vol. 15 No. 2 (2024): December 2024
Section Original Research Articles
DOI: https://doi.org/10.30659/sainsmed.v15i2.33505
Keywords: Peach, Coffee Arabica, Combination, DPPH

Abstract

Antioxidant compounds scavenge unstable free radical reactions and are abundant in certain plants. Peaches (Prunus persica L.) are rich in phytochemicals such as carotenoids, anthocyanins, phenolic acids, and flavonoids, while Arabica coffee (Coffea arabica) contains alkaloids, flavonoids, tannins, steroids, and terpenoids. This study evaluated the antioxidant activity and phytochemical profile of peaches, Arabica coffee, and their combination using ethanol, ethyl acetate, and water as solvents. Extraction was performed via maceration with 96% ethanol and fractionation using water and ethyl acetate. Antioxidant activity was assessed using the DPPH (1,1-Diphenyl-2-picrylhydrazyl) assay, and phytochemical tests evaluated secondary metabolites such as alkaloids, flavonoids, phenolics, steroids/terpenoids, tannins, and saponins. Results indicated that the ethyl acetate extract of peaches showed moderate antioxidant activity (IC50 = 141.91 ppm), while the water extract of Arabica coffee exhibited robust activity (IC50 = 22.30 ppm). Notably, combining water extracts from both sources demonstrated enhanced antioxidant capacity (IC50 = 37.85 ppm), improving peach extract activity by over threefold. These findings underscore the potential synergistic effects of combining these natural sources for health-promoting antioxidants.

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Author Biographies

  • Dhea Angela Putri, Medical Study Program, Faculty of Medicine, YARSI University, Central Jakarta
    Medical Study Program, Faculty of Medicine, YARSI University, Central Jakarta, Indonesia.
  • Himmi Marsiati, Doctoral Program, Biomedical Sciences, YARSI University, Central Jakarta

    Doctoral Program, Biomedical Sciences, YARSI University, Central Jakarta, Indonesia.

License

Creative Commons License

© 2024 Author(s). Published by Sains Medika: Jurnal Kedokteran dan Kesehatan. This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

How to Cite

Putri, D. A., & Marsiati, H. (2024). Antioxidant activity and phytochemical profiling of peaches, Arabica coffee, and their combination. Sains Medika: Jurnal Kedokteran Dan Kesehatan, 15(2), 57-61. https://doi.org/10.30659/sainsmed.v15i2.33505

References

Ajhar, M. N., & Meilani, D. (2020). Skrining fitokimia dan uji aktivitas antioksidan dari ekstrak etanol biji kopi arabika (Coffea arabica) yang tumbuh di Daerah Gayo dengan Metode DPPH. Pharma Xplore, 5(1), 34–40. https://doi.org/10.36805/jpx.v5i1.978

Asra, R., Yetti, D. Y., Misfadhila, S., Audina, S., Agustina, A., & Nessa. (2019). Aktivitas Antioksidan dari ekstrak kering kulit dan daging buah naga (Hylocereus lemairei (Hook) Britton & Rose). Jurnal Farmasi Higea, 11(1):17-22. http://dx.doi.org/10.52689/higea.v11i1.207.

Aziz, S., & Rahman, H. (2013). Biological activities of Prunus persica L. Journal of Medicinal Plants Research, 7(15): 947–951. http://dx.doi.org/10.5897/JMPR12.232

Bintang, M. (2017). Biokimia Teknik Penelitian. Erlangga.Jakarta.

Cahyani, Y. N. (2015). Perbandingan Kadar Fenol total dan aktivitas antioksidan ekstrak metanol daun kopi robusta (Coffea canephora) dan Kopi Arabika (Coffea arabica). Skripsi. Fakultas Farmasi. Universitas Jember.

Dabbou, S. (2017). Carotenoids, phenolic profile, mineral content and antioxidant properties in flesh and peel of Prunus persica fruits during two maturation stage. Plant Foods Hum Nutritiont Journal, 72(1):103–110. https://doi.org/10.1007/s11130-016-0585-y

Dhingra, N., Sharma, R., & Kar, A. (2014). Towards further understanding on the antioxidative activities of Prunus persica fruit: A comparative study with four different fractions. Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy, 132,:582–587. https://doi.org/10.1016/j.saa.2014.05.008

Hamzah, M. H. (2016). Uji aktivitas antioksidan fraksi air dan fraksi eter kombinasi ekstrak metanol daun kopi (Coffea arabica) dan ekstrak metanol daun pandan (Pandanus amaryllifolius) dengan metode DPPH. Skripsi. https://repository.unej.ac.id/handle/123456789/78253

Hasnaenil, & Aminah. (2019). Uji aktivitas antioksidan dan profil fitokimia ekstrak kayu beta-beta (Lunasia amara Blanco.). Jurnal Farmasi Galenika, 5(1): 101–107. https://doi.org/10.22487/j24428744.2019.v5.i1.12404.

Ishak, A. (2018). Analisis fitokimia dan uji aktivitas antioksidan biskuit biji labu kuning (Curcubita sp.) sebagai snack sehat. Skripsi Fakultas Kesehatan Masyarakat Universitas Hasanuddin. Makassar.

Mangiwa, S., & Maryuni, A. (2019). Skrining fitokimia dan uji antioksidan ekstrak biji kopi sangrai jenis arabika (Coffea arabica) asal Wamena dan Moanemani, Papua. Jurnal Biologi Papua, 11(2): 103–109. https://doi.org/10.31957/jbp.925

Mangkasa, M. Y., Johnly, A. R., & Audy, D. W. (2018). Uji fitokimia dan aktivitas antioksidan dari ektrak daun bawang kucai (Allium tuberosum Rottl . Ex Spreng) menggunakan spektrofotometer UV-VIS. Phamacon Jurnal ILmiah Farmasi, 7(4): 12–22. https://doi.org/10.35799/pha.7.2018.21417

Mariyah, Y. (2020). Uji Fitokimia dan Aktivitas Antioksidan Ekstrak Kesambi (Schleichera oleosa (Lour.) Oken) Dengan Pelarut Metanol. Skripsi. Fakultas Sains dan Teknologi Universitas Islam Negeri Mualana Malik Ibrahim. Malang. http://etheses.uin-malang.ac.id/24086/1/16620051.pdf.

Molyneux, P. (2004). The use of the stable free radical diphenylpicryl-hydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin Journal of Science and Technology, 26 (3): 211–219. https://www.thaiscience.info/journals/article/song/10462423.pdf

Purwanto, D., Bahri, S., & Ridhay, A. (2017). Uji aktivitas antioksidan ekstrak buah purnajiwa (Kopsia arborea Blume.). dengan berbagai pelarut. Kovalen. 3(1), 24–32.https://dx.doi.org/10.22487/j24775398.2017.v3.i1.8230

Sasmita, S. O., Purwanti, L., & Sadiyah, E. R. (2014). Perbandingan aktivitas antioksidan ekstrak etanol daun, kulit buah dan biji kopi arabika (Coffea arabica L.) dengan Metode peredaman radikal bebas DPPH. Prosiding Farmasi, 5(2): 699–705. http://dx.doi.org/10.29313/.v0i0.18095

Setiadi, Y. (2016). Uji Aktivitas antioksidan fraksi air dan fraksi eter kombinasi ekstrak metanol daun kopi arabika (Coffea arabica) dan kelopak bunga rosella (Hibiscus sabdariffa). Skripsi Digital Repository Universitas Jember, 141. https://repository.unej.ac.id/handle/123456789/78322

Tristantini, D., Ismawati, A., Pradana, B. T., & Gabriel, J. (2016). Pengujian aktivitas antioksidan menggunakan metode DPPH pada daun tanjung ( Mimusops elengi L ). Prosiding Seminar Nasional Teknik Kimia “Kejuangan”. pages G1-1-G1-7. http://jurnal.upnyk.ac.id/index.php/kejuangan/article/view/1547/1420