COMPARISON OF THE NUMBER OF MACROPHAGES IN THE RAT REVERSIBLE PULPITIS AFTER APPLICATION OF BIODENTIN AND SIWAK EXTRACT

Authors

DOI:

https://doi.org/10.30659/medali.4.3.6-11

Keywords:

macrophages, reversible pulpitis, direct pulp capping, biodentin, siwak extract

Abstract

Background: Oral disease still need special attention, especially in dentistry. Inflammation of the pulp is caused by the presence of irritants. One of the mechanical irritants is iatrogenic factors caused by operator error during preparation so that roof of pulp chamber is exposed. This condition is called reversible pulpitis/inflammation of the pulp. Cells that play a role during inflammation are macrophages which are the second defense cell after neutrophil apoptosis. A direct pulp capping procedure is indicated for this condition. The material commonly used is biodentin, but because the price tends to be expensive, alternative materials are needed. Siwak contains flavonoids which are known to play an important anti-inflammatory role in the healing of exposed pulp. The purpose of this study was to compare the number of macrophages in the dental pulp of rats with reversible pulpitis after administration of biodentin and siwak extract.
Method: This research is a true experimental with a post test only group design, consisting of 2 treatment groups, namely the 75% siwak extract group and the biodentin group. The material is applied after the tooth has been prepared. The research sample used male wistar rats and sacrificed on the 3rd day. Samples were stained with Hematoxylin Eosin to see macrophage cells.
Result: The data obtained were analyzed using the independent T-test. The test results showed a significant difference, with a value of p = 0.008 (p <0.05).
Conclusion: The conclusion of this study that the siwak extract group showed a lower mean number of macrophage cells than the biodentin group.

Author Biographies

  • Andina Rizkia Putri Kusuma, Sultan Agung Islamic University
    Departemen Konservasi Gigi
  • Rahmawati Sri Praptiningsih, Sultan Agung Islamic University
    Departemen Biologi Oral
  • Arlina Nurhapsari, Sultan Agung Islamic University
    Departemen Konservasi Gigi

References

Kemenkes RI, Profil Kesehatan Indonesia 2016. 2011.

J. Ingle, Leif K. Bakland, H. C, and B. J. Craig, “modern endodontic therapy:past ,present and future,†in modern endodontology, 2019, p. 368.

S. Puspita, “Fungsi Jaringan Pulpa dalam Menjaga Vitalitas Gigi,†no. 4, 2010.

E. W. Bachtiar, M. Yuniastuty, A. Monica, and B. M. Bachtiar, “Pengaruh Pemberian Kombinasi Sel Punca Pulpa Gigi dan Recombinant Human Bone Morphogenetic Protein-2 terhadap Kadar Fosfatase Alkali pada Pulpa Gigi Tikus Terinflamasi,†dentika Dent. J., vol. 16, no. 1, pp. 36–40, 2011.

Grossman, “Grossman’s Endodontics Practice 13th Edition,†India: Wolters Kluwer, 2014, p. 576.

N. Fatimatuzzahro, T. Haniastuti, and J. Handajani, “Respon Inflamasi Pulpa Gigi Tikus Sprague Dawley Setelah Aplikasi Bahan Etsa Ethylene Diamine Tetraacetic Acid 19% dan Asam Fosfat 37%,†Dent. J. (Majalah Kedokt. Gigi), vol. 46, no. 4, p. 190, 2013, doi: 10.20473/j.djmkg.v46.i4.p190-195.

Soepribadi, “Regenerasi dan Penyembuhan,†Jakarta: EGC, 2013, p. 12.

I. A. Soedjono, P. Pendidikan, D. Gigi, I. K. Gigi, F. K. Gigi, and U. Indonesia, “Perbandingan Penglepasan Ion Kalsium,†Univ. Indones., 2014.

T. Annisa and A. S. P. Pertiwi, “Biodentine pada Pulpotomi Vital Gigi Sulung,†Indones. J. Paediatr., vol. 1, no. 2, pp. 197–203, 2018.

G. Nayak and M. F. Hasan, “Biodentine- a Novel Dentinal Substitute for Single Visit Apexification,†Restor. Dent. Endod., vol. 39, no. 2, p. 120, 2014, doi: 10.5395/rde.2014.39.2.120.

F. Fatkhurrohman and A. Medawati, “Efektifitas Ekstrak Etanol Kayu Siwak (Salvadora Persica L.) Dengan Metode Perkolasi Terhadap Pertumbuhan Sta phylococcus Aureus Isolat 248 Yang Resisten Multiantibiotik,†Insisiva Dent. J. Maj. Kedokt. Gigi Insisiva, vol. 2, no. 2, pp. 35–42, 2013.

A. Sabir, “THE USES OF FLAVONOIDS IN DENTISTRY.†pp. 81–87, 2013.

S.-J. Kim, M.-H. Lim, I.-K. Chun, and Y.-H. Won, “Effect of Flavonoids of Ginkgo biloba on Proliferation of Human Skin Fibroblast,†Ski. Pharmacol, vol. 10, pp. 200–205, 1997.

F. I. Putri, “Respons Antiinflamasi Ekstrak Kulit Buah Manggis (Garcinia mangostana Linn) Terhadap Jumlah Sel Neutrofil Pada Gingiva Tikus Wistar Jantan Pasca Diinduksi Oleh Porphyromonas gingivalis,†Univ. Jember, 2015.

M. C. Nuria, “Maulita Cut Nuria, dkk Uji Aktivitas Antibakteri .........,†vol. 5, no. 2, pp. 26–37, 2009.

T. U. Sapara and O. Waworuntu, “Efektivitas Antibakteri Ekstrak Daun Pacar Air (Impatiens Balsamina L.) Terhadap Pertumbuhan Porphyromonas Gingivalis,†Pharmacon, vol. 5, no. 4, pp. 10–17, 2016, doi: 10.35799/pha.5.2016.13968.

T. P. T. Cushnie and A. J. Lamb, “Antimicrobial activity of flavonoids,†Int. J. Antimicrob. Agents, vol. 26, no. 5, pp. 343–356, 2005, doi: 10.1016/j.ijantimicag.2005.09.002.

Murray, “Bahan Ajar Uji Bioaktivitas : Antioksidan,†Univ. Udayana, no. April, pp. 1–51, 2009, [Online]. Available: https://simdos.unud.ac.id/uploads/file_pendidikan_1_dir/75b8895f814f85fe9ae5ce91dc5411b1.pdf.

S. H. Narváez and A. L. V. Rodriguez, “Biodentine: Un nuevo material en terapia pulpar / Biodentine: A New Material for Pulp Therapy,†Univ. Odontol., vol. 34, no. 73, pp. 69–76, 2015, doi: 10.11144/javeriana.uo34-73.bmtp.

A. Marapita, A. Rizkia, P. Kusuma, and M. Muhtar, “Perbandingan Jumlah Sel Makrofag Pulpa Gigi Tikus Wistar Setelah Aplikasi Tiga Jenis Medikamen Kaping Pulpa,†Konf. Ilm. Mhs. Unissula 3, pp. 21–28, 2020.

S. Deviyanti, “SEMEN TRIKALSIUM SILIKAT SEBAGAI BAHAN ALTERNATIF UNTUK PENATALAKSANAAN HIPERSENSITIF DENTIN (Kajian Pustaka),†J. Ilm. dan Teknol. Kedokt. Gigi, vol. 13, no. 1, p. 12, 2017, doi: 10.32509/jitekgi.v13i1.852.

D. Mukhtar, “Makrofag Pada Jaringan Adiposa Obes Sebagai Penanda Terjadinya Resistensi Insulin,†Maj. Ilm. Widya, vol. 3, no. 317, pp. 30–31, 2013, [Online]. Available: https://e-journal.jurwidyakop3.com/index.php/majalah-ilmiah/article/view/52.

K. G. Baratawidjaja and I. Rengganis, Imunologi Dasar, XI (cetaka. Jakarta: Fakultas Kedokteran Universitas Indonesia, 2016.

I. D. Sarihati, “Makrofag Dan Aterosklerosis,†Meditory J. Med. Lab., vol. 5, no. 1, pp. 61–67, 2017, doi: 10.33992/m.v5i1.113.

F. M. Siregar, “Immunosenescence : Penuaan Pada Sel Makrofag,†J. Ilmu Kedokt., vol. 13, no. 1, p. 14, 2019, doi: 10.26891/jik.v13i1.2019.14-22.

H. Taurina, “Jumlah Makrofag M1 dan M2 Dan Derajat Histologi Tumor Pada Model Karsinoma Payudara Tikus Sprague Dawley Yang Diinduksi 7,12 Dimethylbenz Anthracene (DMBA),†Dr. Diss. Univ. Gajah Mada, 2016.

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Published

2022-12-12

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Vol. 4: Special Issue 1. Desember 2022

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Articles

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