Combined Effects Of Aloe Vera And Manuka Honey On IL-6 Level In Periodontitis-Induced Rats

Isa Anshori Muchaeroni*  -  Department of Oral Biology Dentistry, Universitas Islam Sultan Agung, Indonesia
Recita Indraswary  -  Department of Oral Biology Dentistry, Universitas Islam Sultan Agung
Anggun Feranisa  -  Department of Oral Biology Dentistry, Universitas Islam Sultan Agung, Indonesia
Rahmawati Sri Praptiningsih  -  Department of Oral Biology Dentistry, Universitas Islam Sultan Agung, Indonesia
Eko Hadianto  -  Departement of Dental Materials, Faculty of Dentistry, Universitas Islam Sultan Agung, Semarang, Indonesia, Indonesia
Irma Dewi Ratnawati  -  Department of Periodontology Faculty of Dentistry Sultan Agung Islamic University, Indonesia
Nindi Rahmawati Santoso  -  Undergraduate Programme, Faculty of Dentistry, Universitas Islam Sultan Agung, Semarang, Indonesia, Indonesia

(*) Corresponding Author
DOI: 10.30659/medali.8.1.25-31

Supp. File(s): Research Instrument

ABSTRACT

Background: Periodontitis is a multifactorial inflammatory condition that progressively damages the tooth-supporting structures, including the alveolar bone and periodontal ligament. This process involves a rise in pro-inflammatory cytokines, such as IL-6, which are essential agents in the inflammation and tissue destruction. Aloe vera (Aloe vera) and manuka honey are recognized for their ability to reduce inflammation and suppress the release of pro-inflammatory signaling molecules, such as IL-6. This study aims to evaluate the effectiveness of the combination of Aloe vera and Manuka honey in reducing IL-6 level in periodontitis rats.

Method: Thirty rats were randomly assigned to four experimental groups: a control group, Aloe vera group, Manuka Honey group, and a group receiving a combination of Aloe vera and Manuka Honey. The Aloe vera and Manuka Honey treatments were administered via a gingival injection at a dose of 150 uL. After five days treatment, the rats were euthanized. An ELISA (Enzyme-Linked Immunosorbent Assay) was  conducted on the gingival tissue to measure the concentration of Interleukin-6 (IL-6).

Result: The combination of Manuka honey and Aloe Vera significantly reduced IL-6 levels compared to the control group (p=0.000).

Conclusion: The combination of Aloe Vera and manuka honey are effectively and  significantly reduced on IL-6 level.

Supplement Files

Keywords: Periodontitis;IL- 6

  1. Kementerian Kesehatan Republik Indonesia (2023) Survei Kesehatan Indonesia 2023: Laporan Nasional. Jakarta: Kemenkes RI.
  2. Hendiani, I. et al. (2021) ‘Effectiveness of Mangosteen (Garcinia Mangostana L.) Peel Gel on the MMP-8 Levels in Chronic Periodontitis Patients after Scaling and Root Planing’, Journal of International Dental and Medical Research, 14(2), pp. 654–659.
  3. Saliem, S.S. et al. (2022) ‘Pathogenesis of periodontitis – A potential role for epithelial-mesenchymal transition’, Japanese Dental Science Review. Elsevier Ltd, pp. 268–278.
  4. Sczepanik, F.S.C. et al. (2020) ‘Periodontitis is an inflammatory disease of oxidative stress: We should treat it that way’, Periodontology 2000, 84(1), pp. 45–68.
  5. Timothy, C.N. and Rajasekar, A. (2020) ‘Efficacy Of Aloe vera gel as an adjunct to scaling and root planing in the management of chronic periodontitis’, Plant cell Biotechnology and Molecular Biology, 21(27–28), pp. 14–20.Chelu et al. (2023). Aloe vera-Based Hydrogels for Wound Healing: Properties and Therapeutic Effects.MDPI. page 1-3
  6. Al-Kubaisi, M.W. et al. (2023) ‘Anti-inflammatory effects of manuka honey on salivary cytokines (clinical study)’, Journal of Baghdad College of Dentistry, 35(1), pp. 2311–5270.
  7. Sęk, A., Porębska, A. and Szczęsna, T. (2023) ‘Quality of Commercially Available Manuka Honey Expressed by Pollen Composition, Diastase Activity, and Hydroxymethylfurfural Content’, Foods, 12(15).
  8. Russell, F.D., Visagie, J.C. and Noll, J.L. (2022) ‘Secretion of IL-6 by fibroblasts exposed to Australian honeys involves lipopolysaccharide and is independent of floral source’, Scientific Reports, 12(1).
  9. Kurnia, E.D., Ratnasari, D. and Helmiawati, Y., 2019. Pembuatan Gel Ekstrak Daun Petai Cina (Leucaena glauca, Benth) Dengan Basis Gel Lidah (Aloe Vera L.) Buaya Sebagai Obat Luka Terbuka. Journal of Holistic and Health Sciences (Jurnal Ilmu Holistik dan Kesehatan), 3(1), pp.39-45.
  10. Muchaeroni, I. A., Rochmah, Y. S., & Putra, A. (2023). The Effect of secretome-hypoxia mesenchymal-stem-cells and vitamin-d3 in type-2 diabetes-mellitus induced periodontitis rats. Odonto: Dental Journal, 10(1), 125-131.
  11. Kurnia, E.D., Ratnasari, D. and Helmiawati, Y., 2019. Pembuatan Gel Ekstrak Daun Petai Cina (Leucaena glauca, Benth) Dengan Basis Gel Lidah (Aloe Vera L.) Buaya Sebagai Obat Luka Terbuka. Journal of Holistic and Health Sciences (Jurnal Ilmu Holistik dan Kesehatan), 3(1), pp.39-45.
  12. Yazdani, N., Hossini, S.E. and Edalatmanesh, M.A. (2022) ‘Anti-inflammatory Effect of Aloe vera Extract on Inflammatory Cytokines of Rats Fed with a High-Fat Diet (HFD)’, Jundishapur Journal of Natural Pharmaceutical Products, 17(1).
  13. Muna, M.A.R. and Yuristra, F.S. (2024) ‘Kajian Potensi Antiinflamasi Tanaman Karamunting (Rhodomyrtus Tomentosa) Terhadap Penyakit Periodontal Gingivitis dan Periodontitis’, Jurnal Pendidikan Tambusai, 8(1), pp. 10271–10276
  14. Hermanto, N.R. and Syaify, A., 2015. Pengaruh Aplikasi Gel Aloe Vera Sebagai Bahan Tambahan Scaling dan Root Planing Terhadap Penyembuhan Jaringan Periodontal Pada Perawatan Periodontitis Kronis (Kajian pada Bleeding On Probing, Pocket Depth, dan Clinical Attachment Level). Jurnal Kedokteran Gigi, 6(3), pp.307-314.
  15. Budai, M.M., Varga, A., Milesz, S., Tőzsér, J. and Benkő, S., 2013. Aloe vera downregulates LPS-induced inflammatory cytokine production and expression of NLRP3 inflammasome in human macrophages. Molecular immunology, 56(4), pp.471-479.
  16. a Schmidlin, P.R., English, H., Duncan, W., Belibasakis, G.N. and Thurnheer, T., 2014. Antibacterial potential of Manuka honey against three oral bacteria in vitro. SWISS DENTAL JOURNAL SSO–Science and Clinical Topics, 124(9), pp.922-4.
  17. Safii, S.H., Tompkins, G.R. and Duncan, W.J. (2017) ‘Periodontal Application of Manuka Honey: Antimicrobial and Demineralising Effects In Vitro’, International Journal of Dentistry, 2017.
  18. Minden-Birkenmaier, B.A. et al. (2019) ‘The effect of manuka honey on dHL-60 cytokine, chemokine, and matrix-degrading enzyme release under inflammatory conditions’, Med one, 4(2).
  19. Putri, C.F. and Bachtiar, E.W. (2020) ‘Porphyromonas gingivalis dan patogenesis disfungsi kognitif: analisis peran sitokin neuroinflamasi’, Cakradonya Dental Journal, 12(1), pp. 15–23.

Lisensi Creative Commons
This work is licensed under a Lisensi Creative Commons Atribusi-BerbagiSerupa 4.0 Internasional.
Contact us: MEDALI Jurnal: Media Dental Intelektual : Jl. Raya Kaligawe Km.4, PO BOX 1054/SM Semarang, Central Java, Indonesia, 50112. Email: odontodentaljournal@unissula.ac.id
apps