INHIBITORY POWER OF PROBIOTICS DRINKS ON THE STREPTOCOCCUS MUTANS GROWTH

Rosida Intan Andica, Welly Anggarani, Andina Rizkia Putri Kusuma

Abstract


Background: Streptococcus mutans is an oral cavity bacteria that is dominantly found. The acidogenic nature of bacteria can affect the damage to the physical and biochemical structures of dental biofilms. Streptococcus mutans growth becomes important to control. One way to control its growth is through the use of probiotic bacteria available in processed foods. The objective of this study is to investigate the effect of probiotic drinks against the Streptococcus mutans mutans.
Method: This study is experimental research with a posttest-only group design, using two test groups. Streptococcus mutans suspension with McFarland turbidity standard of 0.5 in MRS Agar medium was placed on a paper disc that had been soaked in aqua dest (control group) or probiotic drink (treatment group) for ± 20 minutes and then incubated for 48 hours at 37oC. Each group was replicated 16 times. After incubation, the diameter of the Streptococcus mutans growth inhibition zone formed was measured using a caliper.
Result: The control group did not show an inhibitory zone, while the treatment group resulted in a mean diameter inhibition zone of 1.18 mm. The Mann Whitney test proved that the treatment group had a significantly higher diameter of the inhibition zone compared to the control one (p=0.000).
Conclusion: Probiotic drink had an inhibitory power on the Streptococcus mutans growth


Keywords


Probiotic drink; inhibitory power; Streptococcus mutans

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References


Tahir L, Nazir R. Dental Caries, Etiology, and Remedy through Natural Resources. In: Dental Caries - Diagnosis, Prevention and Management.IntechOpen; 2018. p. 19–33.

Soesilawati P. Imunogenik Karies Gigi. Surabaya: Airlangga University Press; 2020. 2 p.

Whawell SA, Lambert DW. Basic Sciences for Dental Students. West Sussex: John Wiley & Sons Ltd; 2018. 116 p.

Alejandra BM, Daniel OM. Virulence Factors of Streptococcus mutans Related to Dental Caries. In: Staphylococcus and Streptococcus. IntechOpen; 2020. p. 137–44.

Putra INK. Substansi Nutrasetikal Sumber dan Manfaat Kesehatan. Yogyakarta: Deepublish Publisher; 2020. 44 p.

Sugiaman VK. Manfaat Keasaman Yogurth dalam Pencegahan Karies Gigi. Zenit J Ilm Univ Kristes Maranatha. 2014;3(2):103–10.

Claudia FSZ, Wilvia W, Nababan I, Erawati S. Pengaruh Berkumur Larutan Probiotik Terhadap Peningkatan pH Saliva pada Anak-Anak di Panti Asuhan Terima Kasih Kota Medan Tahun 2019. Prima JODS. 2019;2(2):2.

Parameswari A, Kuntari S, Herawati. Streptococcus mutans (Growth Inhibition of Probiotics on The Growth of Streptococcus mutans). 2010;2(2)

Kamara DS, Rachman SD, Pasisca RW, Djajasoepena S, Suprijana O, Idar I, et al. Pembuatan dan Aktivitas Antibakteri Yogurt Hasil Fermentasi Tiga Bakteri (Lactobacillus bulgaricus, Streptococcus thermophilus, Lactobacilus acidophilus). Al-Kimia. 2017;4(2):22–32.

Goraya MU, Ashraf M, Ur-Rahman S, Habib A. Short Communication Determination of Antibacterial Activity of Bacteriocins of Lactic Acid. J Infect Mol Biol. 2013;1(1):8–10.

Anggrainy H, Darwin AM. Uji Aktivitas Antibakteri Ekstrak Etanol Batang Inggu (Ruta angustifolia (L.) Pers) Terhadap Streptococcus mutans. Maj Farm. 2017;14(01):49–54.

Aljufri S. Uji Aktivitas dan Mekanisme Penghambatan Antibakteri Ekstrak Air Campuran Daun Sirih (Piper Betle L.) dan Gambir (Uncaria Gambir (Hunter) Roxb.), Terhadap Bakteri Gram Positif. Jurusan Farmasi. Fakultas Kedokteran dan Ilmu Kesehatan UIN Syarif Hidayatullah. UIN Syarif Hidayatullah; 2013.

Wahyuni W, Karim SF. Uji Aktivitas Antibakteri Ekstrak Etanol Daun Kacapiring (Gardenia jasminoides Ellis) terhadap Bakteri Streptococcus mutans. J Sains dan Inform. 2020;4(4):399–404.

Paramita L, Hanum F, Firdausy MD. Perbedaan Efektifitas Antara Keju Cheddar dan Yoghurt Plain Terhadap Pertumbuhan Bakteri Streptococcus Mutans Secara In Vitro. Medali J. 2015;2(1):59–63.

Lestari LA, Helmyati S. Peran Probiotik di Bidang Gizi dan Kesehatan. Yogyakarta: Gadjah Mada University Press; 2018. 72 p.

Nur F. Potensi Bakteri Asam Laktat yang Diisolasi dari Dangke sebagai Kandidat Probiotik dalam Menghambat Bakteri Patogen. Program Pasca Sarjana Unhas. Universitas Hasanuddin; 2017.

Lindawati Y, Simanjuntak DV. Perbedaan nilai pH dan jumlah koloni Streptococcus species sebelum dan setelah mengonsumsi minuman probiotik. Padjadjaran J Dent Res Students. 2020;4(2):115–20.

Glavina D, Gorseta K, Skrinjarić I, Vranić DN, Mehulić K, Kozul K. Effect of LGG yoghurt on Streptococcus mutans and Lactobacillus spp. salivary counts in children. Coll Antropol [Internet]. 2012;36(1):129–32.

Ilyas M, Phielip C. Drinking yogurt reducts colony of cariogenic bacteria in young adults saliva ( Meminum yogurt menurunkan jumlah koloni bakteri kariogenik dalam saliva pada usia remaja ). Dentofasial. 2014;13(1):28–32.

Parasthi LYE, Afifah DN, Nissa C, Panunggal B. Total Lactic Acid Bacteria and Antibacterial Activity in Yoghurt with Addition of Ananas comosus Merr. and Cinnamomum burmannii. Amerta Nutr. 2020;4(4):257.

Mulyawan PH. Pengaruh Berbagai Minuman Probiotik Terhadap Hambatan Pertumbuhan Streptococcus mutans In Vitro. Naskah Publ FK UMS. 2014;1–12.

Corry JEL, Curtis GDW, Baird RM. de man, rogosa and sharpe (MRS) agar. Prog Ind Microbiol. 2003;37:511–3.

Rahman NZA, Hanafiah RM, Ghafar SAA, Abdullah N, Azman NN. Isolation and Antimicrobial Activity of Lactic Acid Bacteria against Streptococcus Mutans. J Int Dent Med Res. 2020;13(2):417–21.




DOI: http://dx.doi.org/10.30659/medali.4.2.97-103

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