INDONESIAN JOURNAL OF SUSTAINABILITY
CRISPR/Cas9-Mediated Editing of OsPYL9 Enhances Drought Tolerance in Rice (Oryza sativa L.) through ABA and Circadian Rhythm Signaling
Abstract
Rice (Oryza sativa L.) is a major staple crop worldwide, yet its productivity is severely constrained by increasing drought stress driven by climate change and water scarcity. Abscisic acid (ABA) signaling plays a central role in regulating plant responses to drought, with the PYR/PYL/RCAR family of ABA receptors acting as key molecular regulators. Among these, OsPYL9 has emerged as an important candidate gene due to its involvement in drought tolerance and circadian rhythm–associated stress regulation. This study aims to enhance drought resilience in rice through precise genome editing of OsPYL9 using CRISPR/Cas9 technology. A locally adapted, high-yielding rice variety will be subjected to targeted gene modification, followed by molecular validation, physiological screening under controlled drought stress, and comprehensive agronomic evaluation. The expected outcomes include improved water-use efficiency, enhanced antioxidant activity, optimized stomatal regulation, and stable grain yield under water-limited conditions. This research provides a sustainable and precise breeding strategy for developing climate-resilient rice varieties and offers significant potential for strengthening food security in drought-prone regions.
Keywords: CRISPR/Cas9, OsPYL9, Drought tolerance, Abscisic acid signaling, Rice genomics
References
References
Gross, B. L., & Zhao, Z. (2014). Archaeological and genetic insights into the origins of domesticated rice. Proceedings of the National Academy of Sciences, 111(17), 6190–6197.
https://doi.org/10.1073/pnas.1308942110
Huang, X., Kurata, N., Wei, X., Wang, Z. X., Wang, A., Zhao, Q., … Han, B. (2012). A map of rice genome variation reveals the origin of cultivated rice. Nature, 490(7421), 497–501.
https://doi.org/10.1038/nature11432
Rashid, A., & Ahmad, Z. (2020). Economic significance of rice crop and its contribution to rural livelihoods in Pakistan. Sarhad Journal of Agriculture, 36(4), 1123–1132.
https://doi.org/10.17582/journal.sja/2020/36.4.1123.1132
Fitzgerald, M. A., McCouch, S. R., & Hall, R. D. (2009). Not just a grain of rice: The quest for quality. Trends in Plant Science, 14(3), 133–139.
https://doi.org/10.1016/j.tplants.2008.12.004
Gao, X., Xie, J., Zhang, Y., & Wang, X. (2020). Rice consumption and its role in global food security and nutrition. Food Security, 12(4), 673–687.
https://doi.org/10.1007/s12571-020-01038-9
Abedullah, N., Khalid, M., & Kouser, S. (2021). Impact of rice production on farm income and rural employment in Pakistan. Journal of the Saudi Society of Agricultural Sciences, 20(4), 273–281.
https://doi.org/10.1016/j.jssas.2021.03.002
Farooq, M., Hussain, M., & Siddique, K. H. M. (2014). Drought stress in wheat during flowering and grain-filling periods. Critical Reviews in Plant Sciences, 33(4), 331–349.
https://doi.org/10.1080/07352689.2014.875291
Shaban, M., Khan, A., & Ahmad, S. (2022). Global rice production, trade, and consumption trends under changing climate. Climate Risk Management, 36, 100423.
https://doi.org/10.1016/j.crm.2022.100423
Hussain, S., Khan, M. A., & Raza, M. A. (2021). Analysis of rice production, export performance and growth trends in Pakistan. Journal of Asian Business and Economic Studies, 28(3), 219–232.
https://doi.org/10.1108/JABES-04-2020-0039
Fidler, A., Biesiada, T., & Borkowski, K. (2022). The PYR/PYL/RCAR family of ABA receptors: Structure, function, and regulation in abiotic stress tolerance. Plant Molecular Biology, 108(1–2), 1–18.
https://doi.org/10.1007/s11103-021-01169-
Wei, H., Wang, X., & Chen, Z. (2023). Circadian regulation of abscisic acid signaling in rice: Functional characterization of ABA receptors and downstream targets. Plant Physiology and Biochemistry, 195, 152–164.
https://doi.org/10.1016/j.plaphy.2023.02.019
Li, J., Manghwar, H., Sun, L., Wang, P., Wang, G., Sheng, H., Zhang, X., & Jin, S. (2022). CRISPR/Cas genome editing and precision plant breeding in agriculture. Frontiers in Genome Editing, 4, 812546.
https://doi.org/10.3389/fgeed.2022.812546
Wei, H., Wang, X., & Chen, Z. (2023). Circadian regulation of abscisic acid signaling in rice: Functional characterization of ABA receptors and downstream targets. Plant Physiology and Biochemistry, 195, 152–164.
https://doi.org/10.1016/j.plaphy.2023.02.019
Fidler, A., Biesiada, T., & Borkowski, K. (2022). The PYR/PYL/RCAR family of ABA receptors: Structure, function, and regulation in abiotic stress tolerance. Plant Molecular Biology, 108(1–2), 1–18.
https://doi.org/10.1007/s11103-021-01169-0
Wei, H., Wang, X., & Chen, Z. (2023). Circadian regulation of abscisic acid signaling in rice: Functional characterization of ABA receptors and downstream targets. Plant Physiology and Biochemistry, 195, 152–164.
https://doi.org/10.1016/j.plaphy.2023.02.019
Fidler, A., Biesiada, T., & Borkowski, K. (2022). The PYR/PYL/RCAR family of ABA receptors: Structure, function, and regulation in abiotic stress tolerance. Plant Molecular Biology, 108(1–2), 1–18.
https://doi.org/10.1007/s11103-021-01169-0
Article Statistic
Abstract view : 178 timesPDF views : 146 times
How To Cite This :
Refbacks
- There are currently no refbacks.
