The Effect of Honey in Second Degree Burn Healing on Wistar Rats (Overview of Angiogenesis and the Number of Fibroblasts)

Ayyasi Izaz Almaz  -  Medicine Faculty, Diponegoro University, Semarang, Indonesia
Ratna Dama Purnawati  -  Medicine Faculty, Diponegoro University, Semarang, Indonesia
Hermawan Istiadi  -  Medicine Faculty, Diponegoro University, Semarang, Indonesia
Neni susilaningsih  -  Medicine Faculty, Diponegoro University, Semarang, Indonesia

(*) Corresponding Author

Introduction: Burn therapy is generally done using a burn ointment. However, honey can also be used as an alternative treatment for burns. Honey contains active flavonoid as an antioxidant and hydrogen peroxide which can stimulate fibroblasts proliferation also angiogenesis on burn healing phase.

Objective: to determine the effect of honey in second degree burn healing from angiogenesis and the number of fibroblasts on Wistar rats.

Methods: This research used a posttest only controlled group design on 15 rats with random selection and divided into 3 groups: H-G was given honey, B-G was given Bioplacenton®, N-G without any treatment after burn was induced. Rats were given a second degree burn in a size of 2 cm x 2cm on the back area and were treated twice a day for 14 days. Burn healing was measured by calculating the observation results of microscopic VEGF expression, the number of neovascularization, and the number of fibroblasts. Hypothesis analysis used Annova post hoc LSD and Mann-Whitney.

Results: The highest mean in VEGF expression and the number of neovascularization was obtained from group B-G followed by group H-G and group N-G. Significant differences in the number of neovascularization between group B-G with group N-G, p<0.05.

Conclusion: Honey can provide a healing effect on the second degree of burns in Wistar rats in terms of the number of neovascularization as well as Bioplacenton®

Keywords: honey, flavonoid, antioxidant, angiogenesis

  1. Barbara, A.B. et al. (2013) Willard and spackman’s occupational therapy. 12 ed. Philadephia: Wolters Kluwer Health.
  2. Boukraâ, L. (2013) Honey in traditional and modern medicine. Boca Raton, FL: CRC Press.
  3. Christy, E. Manyi-Loh, Clarke, A. M. and Ndip, R. N. (2012) “An overview of honey: Therapeutic properties and contribution in nutrition and human health,” African Journal of Microbiology Research, 5(9), hal. 844–852. doi: 10.5897/ajmr10.008.
  4. Dai, T. et al. (2010) “Topical Antimicrobials for Burn Wound Infections,” Recent Pat Antiinfect Drug Discov, 5(2), hal. 124–51.
  5. Darby, I.A. et al. (2016) “The myofibroblast, a key cell in normal and pathological tissue repair,” Cell Mol Life Sci, 73(6), hal. 1145–57.
  6. Dewi, S. P. (2010) Perbedaan Efek Pemberian Lendir Bekicot (Achatina fulica) dan Gel Bioplacenton TM terhadap Penyembuhan Luka Bersih pada Tikus Putih. Surakarta: Fakultas Kedokteran Universitas Sebelas Maret.
  7. Fatmawati, H., Rudijanto, A. and Indra, M. R. (2010) “Pengaruh Likopen terhadap Penurunan Aktivitas Nuclear Factor kappa Beta ( NF-kB ) dan Ekspresi Intracelular Cell Adhesion Molecule-1 ( ICAM-1 ) pada Kultur HUVECs yang Dipapar Leptin The Role Of Lycopene to Nuclear Factor Kappa Beta ( Nf- κ B ) Activities and on Leptin-Induced Endothelial Cell,” 11(2), hal. 143–150.
  8. Frans, J. B. (2013) Sehat dengan Terapi Lebah. Elex Media Komputindo.
  9. Fromm, D. (2011) Principles of Surgery, Archives of Surgery. doi: 10.1001/archsurg.1975.01360120080021.
  10. Halim, A., Singh, K. K. and Sukur, S. (2011) “Evaluations of bacterial contaminated full thickness burn wound healing in Sprague Dawley rats Treated with Tualang honey,” Indian Journal of Plastic Surgery, 44(1), hal. 112. doi: 10.4103/0970-0358.81459.
  11. Kwakman, P. H. S. and Zaat, S. A. J. (2012) “Antibacterial components of honey,” IUBMB Life, 64(1), hal. 48–55. doi: 10.1002/iub.578.
  12. Majtan, J. (2011) “Methylglyoxal - A potential risk factor of manuka honey in healing of diabetic ulcers,” Evidence-based Complement Altern Med, 2011, hal. 5–10.
  13. Mobayyen, M. et al. (2017) “Partial-thickness burn wounds healing by topical treatment,” Medicine, 96(9), hal. e6168. doi: 10.1097/md.0000000000006168.
  14. Munshi, R. et al. (2014) “Exploration of the Angiogenic Potential of Honey Exploration of the Angiogenic Potential of,” Br. J. Pharm. Res., 4, hal. 477–489. doi: 10.9734/BJPR/2014/6930.
  15. Putri, N.A. and Asparini, R.R. (2017) “Peran Madu dalam Menghambat Pertumbuhan Bakeri pada Luka Bakar,” Fakultas Kedokteran Universitas Muhammadiyah Malang, 13(2), hal. 63–68.
  16. Rao, P. V., Salleh, N. and Gan, S. H. (2016) “Biological and therapeutic effects of honey produced by honey bees and stingless bees: a comparative review,” Revista Brasileira de Farmacognosia. Sociedade Brasileira de Farmacognosia, 1(26), hal. 1–8. doi: 10.1016/j.bjp.2016.01.012.
  17. Specht, E. et al. (2015) “Comparison of immunoreactive score, HER2/neu score and H score for the immunohistochemical evaluation of somatostatin receptors in bronchopulmonary neuroendocrine neoplasms,” Histopathology, 67, hal. 368–377. doi: his.12662.
  18. Stephenson, J.A. et al. (2013) “Tumour angiogenesis: a growth area—from John Hunter to Judah Folkman and beyond,” J Cancer Res, 2013(895019), hal. 1–6. doi: 10.1155/2013/895019.
  19. Svendsen, A. J. et al. (2013) “On the Origin of Rheumatoid Arthritis : The Impact of Environment and Genes — A Population Based Twin Study,” Plos One, 8(2), hal. 1–7. doi: 10.1371/journal.pone.0057304.
  20. Syamsuhidayat, R. (2010) Buku Ajar Ilmu Bedah, Edisi III. Jakarta: EGC.
  21. Vinay Kumar, Abdul K. Abbas, Nelson Fausto, J. A. (2012) Tissue renewal, regeneration, and repair. Elsevier Inc.
  22. Wang, N. et al. (2012) “Ellagic acid , a phenolic compound , exerts anti-angiogenesis effects via VEGFR-2 signaling pathway in breast cancer,” Breast Cancer Res Treat, 134, hal. 943–955. doi: 10.1007/s10549-012-1977-9.
  23. Wardhana, A. et al. (2017) “The epidemiology of burns in Indonesia’s national referral burn center from 2013 to 2015,” Burns Open, 1(2), hal. 67–73. doi: 10.1016/j.burnso.2017.08.002.
  24. WHO. (2014). Burns: Violence and injury prevention. Tersedia pada: (Diakses: 3 Maret 2019).
  25. Wu, J. et al. (2012) “NIH Public Access,” Cancer Lett, 308(1), hal. 43–53. doi: 10.1016/j.canlet.2011.04.012.Caffeic.
  26. Zulfitri, A. et al. (2012) “The Effect of extract Binahong leaf gel (Anredera cordifolia) to improve guinea pigs (Cavia cobaya) fibroblast cell and capillaries number over wound healing process after tooth extraction,” Journal Media Oral Biology Dental Journal, 4(2).

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DOI : 10.30659/sainsmed

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