The Effect of Green Tea and Stevia Extract on Liver Histopathology Wistar Rats Type II Diabetes Mellitus

Elmawati Novit, Udadi Sadhana, Endang Mahati


Introduction: Glucose metabolism abnormality on type 2 diabetes mellitus (T2DM) caused liver damage due to dyslipidemia and increasing oxidative stress. Antidiabetic and antioxidants within green tea leaf and stevia leaf extract prevent liver damage due to T2DM complications. Objective: To investigate green tea and stevia leaf extract effects on liver histopathology on T2DM rats.
Methods: This study used Post Test Only Control Group Design. A total of 30 Wistar rats were divided into 5 groups: control group (C-G), normal rats; negative control group (NC-G), T2DM induction; positive control group (PC-G), treated by Metformin; the first treatment group (T1-G), was treated by green tea leaf extract and stevia leaf extract; and the second treatment group (T2-G) was treated by a Metformin also green tea and stevia leaf extract. The treatment was given to subjects for 21 days starting 3 days after the induction of Nicotinamide (NA) and Streptozotocine (STZ). Histopathological assessment of liver cells uses assessment parameters in the form of features of necrosis, vacuolization, sinusoidal dilation and number of inflammatory cells. Hypothesis will be tested by Kruskal-Wallis (p<0,05) dan Mann-Whitney test (P<0,05)
Results: The difference of liver damage in all group shows a significant difference, p<0.05. Significant differences in liver damage scores between the 2 groups there are between the negative control group and the control group (0.031), positive control (0.042), and second treatment group (0.011), and between first treatment group and second treatment group (0.041).
Conclusion: The administration of green tea extract, stevia leaf, and standard drug may reduce liver damage score of Wistar with T2DM


green tea leaf; stevia leaf; liver; T2DM

Full Text:



Assaei, R. et al. (2016) ‘Hypoglycemic Effect of Aquatic Extract of Stevia in Pancreas of Diabetic Rats: PPAR γ -dependent Regulation or Antioxidant Potential’, Avicenna J Med Biotech, 8(2), pp. 65–74.

Bayat, E. et al. (2017) ‘Effect of the Aquatic Extract of Stevia on the Serum Level of Interleukin-6 in Streptozotocin-Nicotinamide Induced Diabetic Rats’, Shiraz E-Medical Journal, 18(2), p. e4501. doi: 10.17795/semj45015.

Bilous, R. and Donelly, R. (2014) Buku Pegangan Diabetes Edisi Ke 4. 4th edn. Edited by N. B. Bariid. Jakarta: Bumi Medika.

Chacko, S. M. et al. (2010) ‘Beneficial effects of green tea: A literature review’, Chinese Medicine, 5(1), p. 13. doi: 10.1186/1749-8546-5-13.

Effendi, A. teruna and Waspadji, S. (2012) Aspek Biomolekular Diabetes Melitus II. Jakarta: Badan Penerbit FKUI.

Ghasemi, A., Khalifi, S. and Jedi, S. (2014) ‘Streptozotocin-nicotinamide-induced rat model of type 2 diabetes (review)’, Acta Physiologica Hungarica, 101(4), pp. 408–420. doi: 10.1556/APhysiol.101.2014.4.2.

Hidayat, R. S. and Napitupulu, R. M. (2015) Kitab Tumbuhan Obat. Jakarta: AgriFlo (Penebar Swadaya Group).

Ilma, W. Z. (2016) Pengaruh Pemberian Ekstrak Teh Hijau (Camellia sinensis L.) Terhadap Kadar Glukosa Darah dan Gambaran Histopatologi Hepar Mencit Diabetes yang Diinduksi Aloksan. Universitas Jember. Available at:

Iman, M. et al. (2017) ‘Potensi Ekstrak Stevia rebaudiana (Bert.) sebagai Penurun Kadar Gula dalam Darah’, Repository Universitas Kristen Satya Wacana, 1(1), pp. 94–98.

Khiraoui, A. et al. (2017) ‘Stevia rebaudiana Bertoni (Honey Leaf): A Magnificent Natural Bio-sweetener, Biochemical Composition , Nutritional and Therapeutic Values’, Journal of Naturan Sciences Research, 7(August), pp. 75–85.

Liu, K. et al. (2013) ‘Effect of green tea on glucose control and insulin sensitivity: a meta-analysis of 17 randomized controlled trials’, The American Journal of Clinical Nutrition, 98(2), pp. 340–348. doi: 10.3945/ajcn.112.052746.

Mohamed, J. et al. (2016) ‘Mechanisms of Diabetes-Induced Liver Damage: The role of oxidative stress and inflammation’, Sultan Qaboos University Medical Journal, 16(2), pp. e132-141. doi: 10.18295/squmj.2016.16.02.002.

Mohd-radzman, N. H. et al. (2013) ‘Potential Roles of Stevia rebaudiana Bertoni in Abrogating Insulin Resistance and Diabetes: A Review’, Evidence-Based Complementary and Alternative Medicine, 2013, pp. 1–10. doi: 10.1155/2013/718049.

Pandey, S. (2018) ‘Morphology, Chemical Composition and Therapeutic Potential Of Stevia Rebaudiana’, Indo American Journal Of Pharmaceutical Sciences (IAJPS), 05(April), pp. 2260–2266. doi: 10.5281/zenodo.1217075.

Raini, M. and Isnawati, A. (2011) ‘Kajian: Khasiat dan Keamanan Stevia sebagai Pemanis Pengganti Gula’, Media Penelitian dan pengembangan Kesehatan, 21(4), pp. 145–156.

Shen, K. et al. (2017) ‘ScienceDirect Eugenosedin-A improves glucose metabolism and inhibits MAPKs expression in streptozotocin / nicotinamide-induced diabetic rats’, Kaohsiung Journal of Medical Sciences. Published by Elsevier Taiwan LLC, pp. 1–8. doi: 10.1016/j.kjms.2017.11.003.

Sherwood, L. (2014) Fisiologi Manusia dari Sel ke Sistem. 8th edn, Fisiologi Manusia dari Sel ke Sistem. 8th edn. Edited by H. octavius Ong, albertus agung Mahode, and D. Ramadhani. Jakarta: EGC.

Sulaiman, H. A. et al. (2012) Buku Ajar Ilmu Penyakit Hati. Jakarta: CV SAGUNG SETO.

Uribe, M. (2012) ‘Role of Oxidative Stress and Molecular Changes in Liver Fibrosis: A Review’, Current Medicinal Chemistry, 19(28), pp. 4850–4860.

World Health Organization (2016) Global Report On Diabetes. WHO Library Cataloguing-in-Publication Data.

World Health Organization (2018) Noncommunicable Diseases Country Profiles 21018. WHO Library Cataloguing-in-Publication Data.


Article Metrics

Metrics Loading ...

Metrics powered by PLOS ALM


  • There are currently no refbacks.

This work is licensed under a Creative Commons Attribution 4.0 International License.
Based on a work at

View My Stats.