Invitro Antioxidant and Cytotoxic Potential of Ficus carica L. and Olea europeae L. Against Cervical Cancer

Naniek Widyaningrum*  -  Pharmacy Program, Faculty of Medicine, Sultan Agung Islamic University, Indonesia
Atina Hussaana  -  Faculty of Medicine, Sultan Agung Islamic University, Semarang, Indonesia
Nesya Ayu Puspitasari  -  Faculty of Medicine, Sultan Agung Islamic University, Semarang, Indonesia

(*) Corresponding Author
DOI: 10.30659/sainsmed.v11i1.7030

INTRODUCTION:Cervical cancer is a malignant infectious disease caused by Human Papilloma Virus (HPV) in the cervix. Fig and olive oil containing flavonoid has been shown to have antioxidant and anticancer activity.

OBJECTIVE:This study aimed to evaluate antioxidant and cytotoxic activity of combined Ficus carica L. and Olea europeae L. against HeLa cervical cancer cells.

METHODS: This is an experimental study with a post-test only control group design. The HeLa cells were divided into 5 groups: fig extract, olive oil, combined fig extract and olive oil (at the ratio of 1:3, 1:1, 3:1), positive control (doxorubicin). The cytotoxic and antioxidant activity were evaluated by using MTT Assay and DPPH, respectively. The cytotoxic results were analyzed using probit and antioxidant activity was analyzed by using linear regression to obtain IC50 values.

RESULTS:The IC50 cytotoxic of fig extract, olive oil, combined fig extract and olive oil (at the ratio of 1:3, 1:1, 3:1) with positive control (doxorubicin) were 13063,915 μg/mL, 679,593 μg/mL, 1562,356 μg/mL, 746,923μg/mL, 563,626 μg/mL and 13,707 μg/mL respectively. The IC50 antioxidant of fig extract, olive oil, and combination of fig extract and olive oil (3:1) was 105.9272 ppm, 23.1276 ppm, and 21,0689 ppm respectively.

CONCLUSION:The combination of fig extract and olive oil (3:1) was shown to have the highest antioxidant and cytotoxic activity against HeLa cervical cancer cells.

Keywords: Cytotoxic; Antioxidant; Fig Extract (Ficus carica L.); Olive Oil (Olea europeae L.); HeLa cells.

  1. Baqutayan, S. M. S. (2012) ‘The effect of anxiety on breast cancer patients’, Indian Journal of Psychological Medicine, 34(2), pp. 119–123. doi: 10.4103/0253-7176.101774.
  2. Cancer Chemoprevention Research Center (2015) Fixed Procedure of Cytotoxic Test MTT Method.
  3. Cancer Foundation of Indonesia (2017) ‘Chant Together Together Fight Cancer’, integrated expectations, pp. 29–31. doi: 10.1111/j.1468-0289.1971.tb01072.x.
  4. Dasar, D. R. K. kesehatan R. (2013) Indonesian Ministry of Health Litbangkes Agency and Target Population Data, InfoDATIN Indonesian Ministry of Health Data and Information Center.
  5. Fitriana, N. A. and Ambarini, T. K. (2012) ‘Phatophysiology: Clinical concepts of disease processes’, Journal of Clinical Psychology and Mental Health, 1(02), pp. 123–129.
  6. Garcia-Davis, S. et al. (2019) ‘Antitumoral Effect of Laurinterol on 3D Culture of Breast Cancer Explants’, Marine Drugs, 17(4), pp. 1–16. doi: 10.3390/md17040201.
  7. Ghanbari, R. et al. (2012) ‘Valuable nutrients and functional bioactives in different parts of olive (Olea europaea L.)-A review’, International Journal of Molecular Sciences, 13(3), pp. 1291–1340. doi: 10.3390/ijms13033291.
  8. Jasmine, R., Manikandan, K. and Karthikeyan (2015) ‘Evaluating the antioxidant and anticancer property of Ficus carica fruits’, African Journal of Biotechnology, 14(7), pp. 634–641. doi: 10.5897/ajb2014.13742.
  9. John, B. et al. (2014) ‘Total phenolics and flavonoids in selected medicinal plants from Kerala’, International Journal of Pharmacy and Pharmaceutical Sciences, 6(1), pp. 406–408.
  10. John, B., Reddy, V. R. K. and Sulaiman, C. T. (2013) ‘Total phenolics and flavonoids in selected Justicia species’, Journal of Pharmacognosy …, 2(4), pp. 51–52.
  11. Joseph, B. and Justin Raj, S. (2011) ‘Pharmacognostic and phytochemical properties of Ficus carica linn - An overview’, International Journal of PharmTech Research, 3(1), pp. 8–12.
  12. Kichaoui, A. Y. El, Ayesh, B. M. and Aliwaini, S. H. (2016) ‘The use of some plant extracts as an alternative approaches for treatment of certain malignant cell lines’, Journal of medicinal plant studies, 4(4), pp. 174–188.
  13. Lisi, A. K. F., Runtuwene, M. R. J. and Wewengkang, D. S. (2017) ‘Phytochemical Test and Antioxidant Activity of Soyogic Flower Methanol Extract (DC Saurauia bracteosa)’, Pharmacon - the Scientific Pharmacy journal, 6(1), pp. 53–61.
  14. Lui, V. W. Y. and Grandis, J. R. (2012) ‘Primary Chemotherapy and Radiation as a Treatment Strategy for HPV-Positive Oropharyngeal Cancer’, Head and Neck Pathology, 6(SUPPL. 1), pp. 91–97. doi: 10.1007/s12105-012-0364-5.
  15. Ministry of Religion of the Republic of Indonesia (2016) Al-Qur’an. CV. Toha Putra.
  16. Munte, L., Runtuwene, M. R. and Citraningtyas, G. (2015) ‘Antioxidant Activity From Prasman Leaf Extract (Eupatorium triplinerve Vahl.)’, Pharmacon, 4(3), pp. 41–50.
  17. Suttirak, W. and Manurakchinakorn, S. (2014) ‘In vitro antioxidant properties of mangosteen peel extract’, Journal of Food Science and Technology, 51(12), pp. 3546–3558. doi: 10.1007/s13197-012-0887-5.
  18. Tiwary, B. K. et al. (2015) ‘The in vitro cytotoxic activity of ethno-pharmacological important plants of Darjeeling district of West Bengal against different human cancer cell lines’, BMC Complementary and Alternative Medicine, 15(1), pp. 1–10. doi: 10.1186/s12906-015-0543-5.
  19. Wahyuni, S. et al. (2015) Cancer, Top Killer., Mediakom Ministry of Health RI’.
  20. Yao, J. et al. (2014) ‘Oleuropein induced apoptosis in Hela cells via a mitochondrial apoptotic cascade associated with activation of the c-Jun NH2-terminal kinase’, Journal of Pharmacological Sciences, 125(3), pp. 300–311. doi: 10.1254/jphs.14012FP.

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ISSN: 2339-093X (Online) | 2085-1545 (Print)
DOI : 10.30659/sainsmed

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