Probiotics Effect on Interleukin-4 (IL-4) and Imunoglobulin E (IgE) Levels on Asthmatic Patients

Pujiati Pujiati*  -  Pediatrics Departement of Medical Faculty of Sultan Agung Islamic University, Indonesia

(*) Corresponding Author
DOI: 10.30659/sainsmed.v6i2.604

Introduction: Probiotics may play a role in immune system maturation and may reduce the risk of allergies and asthma in childhood. However, the therapeutic benefits of probiotics in asthma depend on various factors such as strain of probiotics and dosing regimen. Objectives: The aims of this study was to evaluate the effect of probiotic (LactoB®) on Immunoglobulin E (IgE) and Interleukin (IL-4) serum level in childhood Asthma.
Methods: Forty children aged 1-5 years with asthma were recruited into a randomized controlled trial. The children were assigned into a probiotic (Lactobacillus acidophilus, Bifidobacterium longun, Streptococcus; Lacto B®) or an equivalent volume of placebo, twice daily orally for 8 weeks. The IgE and IL-4 serum level were determined by ELISA. The differences between groups were analyzed by t-test dependent with confidence interval of 95%.
Results: In intervention group, mean of IgE serum levels after the probiotics treatment was significantly lower compared to that of before the treatment (148.18 pg/mg; 283.20 pg/mg; respectively). Mean of IL-4 serum levels after the treatment was significantly lower compared with that of before the treatment (111.03 pg/mg; 142.08 pg/mg respectively). In control group, there were no significant differences between IgE serum levels mean before the administration of placebo and after the intervention (292.39 pg/ml; 286.94 pg/ml respectively). There were no significant differences between IL-4 serum levels mean before and after the treatment (136.76 pg/ml; 139.56 pg/ml).
Conclusion: there was an effect of probiotics supplementation on IgE and IL-4 serum levels in childhood asthma.

Keywords: Probiotic; Immunoglobulin E.; Interleukin (IL)-4; Childhood Asthma

  1. Abbas A K., Lichtman A H., Pober J S., Cellular and Molecular Immunology, 7th ed. Philadelphia. WB Saunders and Company. 2012.
  2. Baken K A, Ezendam J., Gremmer E R., et al., Evaluation of Immunomodulation by Lactobacillus casei Shirota: Immune Function, Autoimmunity and Gene Expression. Int J of Food Microbiology. 2006, 112: 8-18
  3. Bousquet J., Van Cauwenberge P., Khaltaev N., Allergic Rhinitis and Its Impact on Astham. J Allergy Clin Immunol 2001: 108 Suppl. 5: 8147-334
  4. Cha KB, Mun Jung, Hwan Choi, Song ID, Woong LH, Joon KH, Hyuk J, Kyung CS, Kim K, Chung WS, Seo JG. The effect of a multispecies probiotic mixture on the symptoms and fecal microbiota in diarrhea-dominant irritable bowel syndrome: a randomized, double-blind, placebo-controlled trial. J Clin Gastroenterol. 2012 Mar;46(3):220-7. doi: 10.1097/MCG.0b013e31823712b1.
  5. Hoecke H V., Vandelbulcke L., Cauwenberge P V., Histamin and Leukotrine Receptor Antagonism in The Treatment of Allergic Rhinitis an update. Drugs, 2007, 67(18): 2717-26
  6. Kim JY1, Choi YO, Ji GE. Effect of oral probiotics (Bifidobacterium lactis AD011 and Lactobacillus acidophilus AD031) administration on ovalbumin-induced food allergy mouse model. J Microbiol Biotechnol. 2008 Aug;18(8):1393-400.
  7. Johansson SG., Bieber T., Dahl R., et al., Revised Nomenclature for Allergy for Global Use: Report Nomenclature Review Committee of World Allergy Organization, October 2003, J Allergy Clin immunol 2004;113(5):832-6
  8. Matsuzaki, T., Saito, M., Usuku, K., Nose, H., Izumo, S., Arimura, K., Osame, M., 2005. A prospective uncontrolled trial of fermented milk drink containing viable Lactobacillus casei strain Shirota in the treatment of HTLV-1 associated myelopathy/tropical spastic paraparesis. Journal of the Neurological Sciences 237, 75–81.
  9. Nagao, F., Nakayama, M., Muto, T., Okumura, K., 2000. Effects of a fermented milk drink containing L.casei Shirota on Immune System in Healthy Human Subjects. Bioscience, Biotechnology, and Biochemistry 64,2706-2708.
  10. Tsai CC, Po-Chiang Ke, Ten-Ken Hsu, You-Miin Hsieh. Oral administration of multiple lactic acid bacteria strains suppressed allergic responses IgE in an ovalbumin-induced allergy BALB/c mouse model. AJMR. Vol.6(6), pp. 1206-1212, February 2012
  11. DOI: 10.5897/AJMR11.1430
  12. Torii A1, Torii S, Fujiwara S, Tanaka H, Inagaki N, Nagai H. Lactobacillus Acidophilus strain L-92 regulates the production of Th1 cytokine as well as Th2 cytokines. Allergol Int. 2007 Sep;56(3):293-301. Epub 2007 Aug 1.
  13. Widjaja JT, Diana KJ, Rina LR., Analisis Kadar Interferon Gamma Pada Penderita Tuberkulosis Paru dan Orang Sehat. J Respir Indo Vol. 30, No. 2, April 2010
  14. Kips JC, Tournoy KG, Pauwels RA., New anti-asthma therapies: suppression of the effect of interleukin (IL)-4 and IL-5. European Respiratology Journal. Vol 17 (3). 2001.
  15. Wang M F., et al., Treatment of Perennial Allergic Rhinitis with Lactic Bacteria. Pediatr Allergy Immunol. 2004, 15(2):152-8

Open Access Copyright (c) 2015 Sains Medika : Jurnal Kedokteran dan Kesehatan
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

Sains Medika: Jurnal Kedokteran dan Kesehatan
is published by Faculty of Medicine Universitas Islam Sultan Agung, Indonesia in association with FOKI (FORUM KEDOKTERAN ISLAM INDONESIA).

Contact: Jl. Raya Kaligawe Km.4, PO BOX 1054/SM Semarang 50112, Indonesia
Phone: +62 8122-9933-369
Website: https://fkunissula.ac.id
Email: sainsmedika@unissula.ac.id

ISSN: 2339-093X (Online) | 2085-1545 (Print)
DOI : 10.30659/sainsmed

This work is licensed under a Creative Commons Attribution 4.0 International License

Get a feed by atom here, RRS2 here and OAI Links here

apps