Effect of White Turmeric Extract (Curcuma zedoaria) Using Zam-zam Solvent Compare with Ethanol Solvent Against Breast Cancer Cell T47D

Isna Hudaya, Taufiqurrahman Nasihun, Titiek Sumarawati


Introduction: Curcuma zedoaria is one of the herbal plants potentially protects and cures many diseases, particularly as anti-cancer and anti-tumor. Anti-cancer active compounds of it are flavonoid, phenolic, and saponin. Objectives: This study aimed to explore the cytotoxicity of Curcuma zedoria extract (CZE).
Methods: Experimental Quasi with post test non equivalent control group design on T47D cell line at Biology laboratory, Faculty of Medicine, Sultan Agung Islamic University, Semarang. The research was consisted of 2 groups, namely: intervention group with CZE zam-zam solvent and intervention group with CZE ethanol solvent given 10 different dosages each for 1.000 μg/mL; 500 μg/mL; 250 μg/mL; 125 μg/mL; 62.5 μg/mL; 31.25 μg/mL; 15.62 μg/mL; 7.81 μg/mL; 3.90 μg/mL; 1.95 μg/mL. Cytotoxicity test of IC50 using direct counting method and analyzed by probit analysis.
Results: IC50 value of CZE in zam-zam and ethanol solvent were 28.24 μg/ml and 13.71 µg/ml respectively at the same 24 hours incubation period. Chi-square test revealed p value was 0.000 (α = 0.05), meaning that there was significant relationship.
Conclusion: CZE activity using ethanol is highly active while CZE using zam-zam water is moderate and both of them have the toxicity on breast cancer cell. As the result, apoptosis process may occur.


T47D; Cytotoxicity; Curcuma, Zedoaria; Zam-zam

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Baratawidjaya, K. G. R. I., 2012. Imunologi Dasar Edisi ke 10. Badan Penerbit Fakultas Kedokteran Universitas Indonesia; Jakarta. hal 469-475.

Hiroko D, Teruhiko F, Shino N, Toshihiro K, Kazuo S., 2002. Analisys of Cell growth inhibitory effect of cathechin through MAPK in human breast cancer cell-line T47D. Int J. Oncol.

Kampa, M., Alexaki, I.V., Notas, G., Nifli, P.A., Nistikaki, A., Hatzoglou, A., Bakogeorgou, E., Kouimtzoglou, E., Blekas, G., Boskou, D., Gravanis, A., Castanas, E., 2004. Antiproliferative and Poptotic Effects of Selective Phenolic Acids on T47D Human Breast Cancer Cells: Potential Mechanisme of Action, Breast Cancer Res, 6(2):R63- R74.

Ma’at, Dr. Drs. MS., Apt. Suprapto., 2011. Teknik dasar Kultur Sel. Airlangga University Press. Surabaya.

Manuaba dan Tjakra W, 2010. Panduan Penatalaksanaan Kanker Solid PERBOI 2010. Sagung Seto. Denpasar. hal 17-47.

Peter Boyle and Bernard Levin, 2008. World Cancer Report 2008. Published by the International Agency for Research on Cancer,150 cours Albert Thomas, 69372 Lyon Cedex 08, France. hal 5.

RS Dharmais, 2009. Pasien dan Informasi Kanker. RS.Dharmais Pusat Kanker Nasional. http://www.dharmais.co.id/inde.php/kemoterapi.html (accessed on October 5th, 2014).

Tanamatayarat, P., Limtrakul, P. Chunsakaow, S., Duangrat, C., 2003, Screening of some rubiaceous Plant for Cytotoxic Activity Against Cervix Carcinoma (KB-3-1) Cell line, Thai J,. Pharm, Sci. 27 (3-4): 167-172

DOI: http://dx.doi.org/10.26532/sainsmed.v6i2.601

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