Effect of White Turmeric Extract (Curcuma zedoaria) Using Zam-zam Solvent Compare with Ethanol Solvent Against Breast Cancer Cell T47D

Authors

Published 2016-01-04
Issue Vol. 6 No. 2 (2015): December 2015
Section Original Research Articles
DOI: https://doi.org/10.30659/sainsmed.v6i2.601
Keywords: T47D, Cytotoxicity, Curcuma, Zedoaria, Zam-zam

Abstract

Introduction: Curcuma zedoaria is one of the herbal plants potentially protects and cures many diseases, particularly as anti-cancer and anti-tumor. Anti-cancer active compounds of it are flavonoid, phenolic, and saponin. Objectives: This study aimed to explore the cytotoxicity of Curcuma zedoria extract (CZE).
Methods: Experimental Quasi with post test non equivalent control group design on T47D cell line at Biology laboratory, Faculty of Medicine, Sultan Agung Islamic University, Semarang. The research was consisted of 2 groups, namely: intervention group with CZE zam-zam solvent and intervention group with CZE ethanol solvent given 10 different dosages each for 1.000 ÃŽ¼g/mL; 500 ÃŽ¼g/mL; 250 ÃŽ¼g/mL; 125 ÃŽ¼g/mL; 62.5 ÃŽ¼g/mL; 31.25 ÃŽ¼g/mL; 15.62 ÃŽ¼g/mL; 7.81 ÃŽ¼g/mL; 3.90 ÃŽ¼g/mL; 1.95 ÃŽ¼g/mL. Cytotoxicity test of IC50 using direct counting method and analyzed by probit analysis.
Results: IC50 value of CZE in zam-zam and ethanol solvent were 28.24 ÃŽ¼g/ml and 13.71 µg/ml respectively at the same 24 hours incubation period. Chi-square test revealed p value was 0.000 (ÃŽ± = 0.05), meaning that there was significant relationship.
Conclusion: CZE activity using ethanol is highly active while CZE using zam-zam water is moderate and both of them have the toxicity on breast cancer cell. As the result, apoptosis process may occur.

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© 2016 Author(s). Published by Sains Medika: Jurnal Kedokteran dan Kesehatan. This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

How to Cite

Hudaya, I., Nasihun, T., & Sumarawati, T. (2016). Effect of White Turmeric Extract (Curcuma zedoaria) Using Zam-zam Solvent Compare with Ethanol Solvent Against Breast Cancer Cell T47D. Sains Medika: Jurnal Kedokteran Dan Kesehatan, 6(2), 52-55. https://doi.org/10.30659/sainsmed.v6i2.601

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