Propolis Extracted Using CMCE Decreases TNF–α and C-Reactive Protein level of CCl4- Induced Liver Damage in Rats

Arief Adi Saputro*  -  Universitas Islam Sultan Agung, Indonesia
Taufiqurrachman Nasihun  -  Department of Biocemistry,Faculty of Medicine, Universitas Islam Sultan Agung, Indonesia
Atina Hussaana  -  Department of Biocemistry,Faculty of Medicine, Universitas Islam Sultan Agung, Indonesia

(*) Corresponding Author
DOI: 10.30659/sainsmed.v12i1.5467

Introduction: Carbon tetrachloride (CCl4) is a hepatotoxic substance inducing inflammation in liver cells. Inflammation is marked by increase in proinflammatory cytokines level such as tumor necrosis factor alpha (TNF-α), and C-reactive protein (CRP) produced by hepatocytes. Propolis extracted using continuous multi-stage countercurrent Extraction (CMCE propolis extract) contains active balsamic, caffeic acid phenetyl ester (CAPE), and flavonoids capable of affecting pro-inflammatory cytokines. Objective: to investigate the effect of CMCE propolis extract on TNF-α and CRP levels in CCl4-induced liver damage.
Methods: In this study using a post test only control group design, 28 male Wistar rats were divided into four groups. The control group (C-G) was given CCl4 0.4 ml/200 g on day 14. The P3-G, P7-G, P14-G groups were treated with 3.6 mg/200 g, 7.2 mg/200 g, 14.4 mg/200 g CMCE propolis extract for 14 days and given CCl4 0.4 ml/g on day 14. The TNF-α and CRP levels were assessed using the ELISA kit one-way ANOVA and Post Hoc test (LSD) were applied for the statistical analysis.
Results: TNF-α level in P14-G group (3.26 ± 0.93), P7-G (6.17+0.78), and P3-G (10.4±1.34) were significantly lower than that of C-G (17.29+1.56) (p<0.05). CRP levels in P14-G group (0.61±0.09), P7-G (1.38+0.48), and P3-G (12.01±0.65) were significantly lower than those of C-G (2.95+0.668) (p<0.05). The greatest reduction in TNF-α & CRP level was found in the group given propolis at a dose of 14.4 mg/200 g.
Conclusion: The administration of CMCE propolis extract reduces TNF-α & CRP levels in CCl4-induced liver damage.

Keywords: Propolis extract (CMCE method); TNF-α levels; CRP levels

  1. Aeni, M. N., Kurniawan, I. and Sinaga, H. (2018) ‘Pemeriksaan HbsAg Siswa/Siswi SMA N 1 Pumu Tanjung Sakti Kabupaten Lahat Untuk Kualitas Kesehatan Remaja’, Jurnal Abdimas Musi Charitas, 2(2), pp. 36–40.
  2. Ahmed, K. M. et al. (2012) ‘Anti-inflammatory effect of different propolis extracts in thioacetamide-induced hepatotoxicity in male rat’, Australian Journal of Basic and Applied Sciences, 6(6), pp. 29–40.
  3. Akbari, A. R. and Hestianah, E. P. (2013) ‘Pengaruh Pemberian Propolis Terhadap Gambaran Histopatologi Hepar Mencit (Mus Muscullus) Jantan’, Journal of Chemical Information and Modeling, 53(9), pp. 1689–1699.
  4. Alamudi, M. Y., Hadi, M. I. and Kumalasari, M. L. F. (2018) ‘HbsAg screening in teenagers in Surabaya by using rapid test skrining Surabaya dengan menggunakan rapid test’, Jurnal Kesehatan Masyarakat, 1(3), pp. 30–33.
  5. Annis, F., Purnomo, H. and Balafif, F. (2017) ‘Pengaruh Ekstrak Propolis terhadap Apoptosis melalui Ekspresi Brain-Derived Neurotrophic Factor ( BDNF ) pada Sel Otak Tikus Model Cedera Otak Traumatik The Effect of Propolis Extract Administration on Apoptosis through Brain-Derived Neurotropic Factor ( ’, Jurnal Kedokteran Brawijaya, 29(3), pp. 209–215.
  6. Arief, M. et al. (2014) ‘Hubungan Obesitas dan Hipertrigliseridemia dengan Risiko Perlemakan Hati pada Pasien di Makassar’, Indonesian Journal of Clinical Pharmacy, 1(4), pp. 0–0.
  7. Bhadauria, M. (2012) ‘Propolis prevents hepatorenal injury induced by chronic exposure to carbon tetrachloride’, Evidence-based Complementary and Alternative Medicine, 2012, pp. 1–12. doi: 10.1155/2012/235358.
  8. Damayanti, R., Fitri, L. E. and Dalhar, M. (2016) ‘Pengaruh Pemberian Propolis terhadap Ekspresi INOS dan Kadar MDA pada Otak Tikus Model Cedera Otak Traumatik The Influence of Propolis Administration on iNOS Expression and MDA Level in Rats ’ Brain Model of Traumatic Brain Injury’, Jurnal Kedokteran Brawijaya, 29(2), pp. 110–116. doi: 10.21776/ub.jkb.2016.029.02.3.
  9. Delfanti, R. L. et al. (2018) ‘Analisia High Sensitivity C-Reaktif Protein (hs-CRP) pada Perokok Aktif dengan Faktor Risiko Penyakit Jantung Koroner (PJK) di Warung Kopi Jalan Titipapan Medan Deli’, New England Journal of Medicine, 372(2), pp. 2499–2508.
  10. Dewi, A. C., Ali, M. and Purnomo, H. (2016) ‘Efek Ekstrak Propolis terhadap Ekspresi TNF- α, Apoptosis dan Nekrosis Jaringan Otak Tikus Model Traumatic Brain Injury ( TBI ) The Effects of Propolis Extract on Brain TNF- α Expression, Apoptosis and Necrosis in Rat Model of Traumatic Brain Injury’, Jurnal Kedokteran Brawijaya, 29(2), pp. 117–124.
  11. Herwana, Y. E., Marwoto, W. and Setiabudy, R. (2011) ‘Efek schizandrine C terhadap kerusakan hati akibat pemberian parasetamol pada tikus’, Universa Medicina, 24(4), pp. 161–166.
  12. Ingle, P. V. and Patel, D. M. (2011) ‘C- Reactive Protein in Various Disease Condition - an Overview’, Asian Journal of Pharmaceutical and Clinical Research, 4(1), pp. 9–13. Available at: http://www.embase.com/search/results?subaction=viewrecord&from=export&id=L361820446%0Ahttp://www.ajpcr.com/Vol4Issue1/190.pdf.
  13. Kementerian Kesehatan RI (2013) ‘Riset Kesehatan Dasar (National Health Survey)’, Ministry of Health Republic of Indonesia, 1, pp. 1–303. doi: 10.1007/s13398-014-0173-7.2.
  14. Krisnansari, D., Sulistyo, H. and Ati, V. R. B. (2014) ‘Efek Propolis Terhadap Fungsi Dan Perlemakan Hati (Rattus norvegicus) Model Hiperkolesterolemia (The Effect of Propolis On Liver Function and Fatty Liver of Hypercholesterolemic Rat Model)’, Penel Gizi Makan, 37(1), pp. 77–85.
  15. Lee, S. et al. (2003) ‘In vivo anti-oxidant activities of tectochrysin’, Archives of Pharmacal Research, 26(1), pp. 43–46. doi: 10.1007/BF03179930.
  16. Mihai, C. M. et al. (2011) ‘Correlation between Polyphenolic Profile and Antioxidant Activity of Propolis from Transylvania’, Animal Science and Biotechnologies, 44(2), pp. 100–103.
  17. Mustafiah, S. E., Fatmawati, D. and Yusuf, I. (2011) ‘Indeks Daya Fagosit Makrofag Peritoneum setelah Pemberian Propolis pada Mencit (Mus musculus)’, Sains Medika, 3(2), pp. 121–128.
  18. Prasetyo, D. H., Suparyanti, E. L. and Guntur, A. (2013) ‘Ekstrak Etanol Propolis Isolat Menurunkan Derajat Inflamasi dan Kadar Malondialdehid pada Serum Tikus Model Sepsis Ethanol extract of Propolis Reduces the Level of Inflammation and Serum Malondialdehyde in Sepsis Rats Model’, MKB, 45(3), pp. 161–166.
  19. Soroy, L. et al. (2014) ‘The effect of a unique propolis compound (propoelixTM) on clinical outcomes in patients with dengue hemorrhagic fever’, Infection and Drug Resistance, 7, pp. 323–329. doi: 10.2147/IDR.S71505.
  20. Tatukude, R. L., Loho, L. and Lintong, M. P. (2014) ‘Gambaran histopatologi hati tikus wistar yang diberikan boraks’, Jurnal e-Biomedik (eBM), 2(November).
  21. WHO (World Health Organization) (2018) Liver Disease. doi: 10.7860/JCDR/2015/11423.5658.

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ISSN: 2339-093X (Online) | 2085-1545 (Print)
DOI : 10.30659/sainsmed

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