The Effect of CMCE Propolis Extract Administrations on Interleukin-1 (IL-1) Levels and the Hepatocyte HistopathologicalFindings of Rats’ Induced with CCl4

Catur Retno Lestari, Titiek Sumarawati, Taufiqurrachman Nasihun

Abstract


Introduction:Liver exposure to toxic substances will disrupt the metabolic system. Carbon tetrachloride (CCl4) is a hepatotoxic substance causing a liver damage. Continuous multi-stage countercurrent extraction(CMCE) propolis extract contains active balsamic substances, caffeic acid, phenethyl esters and flavonoids.

Objective: to prove the effect of CMCE propolis extract on IL-1 levels and the hepatocyte histopathology findingsof male wistar rats’induced by CCl4.

Methods:In the posttest only control group design, the total sample of 28 rats was divided into 4 groups: positive control group (K-0; standard feed); group P-1 (propolis3.6 mg/200 g); P-2 (propolis7.2mg/200gr and P-3 (propolis14.4mg/200g) were respectively given for 14 days,yet those on the 14thday were given CCl4. The IL-1 level was measured with the ELISA method, while the male wistar rats’hepatocyte histopathology findings was determined by the HE staining methods. IL-1 levelswere analyzed usingOne Way Anova test,followed by the Post Hoc LSD test. Hepatic cell histopathology was analyzed usingKruskal Wallis, followed by Mann-Whitney U.

Results:Post Hoc LSD analysis shows that the levels of IL-1 in P-1 (983.54 ±19.67), P-2 (841.58±19.47), and P-3 (755.50±31.63) are significantlylower thanthosein K-0 (1131.69±14.91) withp<0.05. Mann-Whitney U analysis shows that the hepatocyte histopathology in the P-1 group (2.55±0.39), P-2 (1.24±0.94) and P-3 (1.20±0.93) is significantlylower thanthatinK-0 (2.94±0.16) with p<0.05.

Conclusion: The administration of CMCE propolis extract may reduce IL-1 levels and improve the male wistar rats’hepatic cell histopathologyinduced with carbon tetrachloride (CCl4).


Keywords


CMCE Propolis extract, IL-1, histopathology hepatic cell

Full Text:

PDF

References


Akbari, A. R. 2015. pengaruh Pemberian Propolis Terhadap Gambaran Histopatologi Hepar Pada Mencit (Mus musculus) Jantan (Doctoral Dissertation, Universitas Airlangga).

Alvina, A. 2016. Type IV collagen as marker of fibrosis in nonalcoholic liver disease. Universa Medicina, 29(2), 114-122.

Annis, F., Purnomo, H. and Balafif, F. 2017. Pengaruh Ekstrak Propolis terhadap Apoptosis melalui Ekspresi Brain-Derived Neurotrophic Factor ( BDNF ) pada Sel Otak Tikus Model Cedera Otak Traumatik The Effect of Propolis Extract Administration on Apoptosis through Brain-Derived Neurotropic Factor, 29(3), pp. 209–215.

Avitsur R; Hunzeker; J, Sheridan, J F. 2006. Role of early stress in the individual differences in host response to viral infection. Brain, Behavior, and Immunity 20: 339-348.

Bagus, I. and Winaya, O. 2011. Kadar Perasan Kunyit yang Efektif Memperbaiki Kerusakan Hati Mencit yang Dipicu Karbon Tetrachlorida, 12(1), pp. 34–39.

Basu, S. 2013. Carbon tetrachloride-induced lipid peroxidation : eicosanoid formation and their regulation by antioxidant nutrients, 189. doi: 10.1016/S0300-483X(03)00157-4.

Bhadauria, M. 2012. Propolis prevents hepatorenal injury induced by chronic exposure to carbon tetrachloride’, Evidence-based Complementary and Alternative Medicine, 2012. doi: 10.1155/2012/235358.

Boll M, Weber LWD, Becker E, Stampfl A. Mechanism of Carbon Tetrachloride-Induced Hepatotoxicity.

Hepatocellular Damage by Reactive Carbon Tetrachloride Metabolites. 2001. Naturforsch. 56c 6 49-659.

D, A. C., Ali, M. and Purnomo, H. 2016. Efek Ekstrak Propolis terhadap Ekspresi TNF- α , Apoptosis dan Nekrosis Jaringan Otak Tikus Model Traumatic Brain Injury ( TBI ) The Effects of Propolis Extract on Brain TNF- α Expression , Apoptosis and Necrosis in Rat Model of Traumatic Brain Injury, 29(2), pp. 117–124.

De Groot H., Littauer A., Hugo-Wissemann D., Wis­semann P. and Noll T. (1988), Lipid peroxidation and cell viability in isolated hepatocytes in a redesigned oxystat system: Evaluation of the hypothesis that lipid peroxidation , preferentially induced at low oxygen partial pressure, is decisive for CC14 liver cell injury. Arch. Biochem. Biophys. 264, 591-599.

Krisnansari, D., Sulistyo, H., & Kusdaryantoi, W. D. 2014. Potensi Hepatoprotektor Propolis Terhadap Hepar Tikus Putih (Rattus Norvegicus) Yang Diinduksi Karbon Tetrakhlorida. Jurnal Ners, 9, 270-78.

Hoesada, I., Nasihun, T., & Isradji, I. 2016. The Effect of Propolis Extract on MDA Levels (Malondialdehyde) and Sperm Quality on Epididimis (Experimental Study on Wistar Strain Male Rats Exposed to Kretek Cigarettes). Sains Medika: Jurnal Kedokteran dan Kesehatan, 7(1), 09-14.

Eteraf-Oskouei, T. and Najafi, M. 2013. Traditional and modern uses of natural honey in human diseases: A review, Iranian Journal of Basic Medical Sciences, 16(6). doi: 10.22038/IJBMS.2013.988.

Fatima, A. et al. 2017. Comparison of Terlipressin with Octreotide in Management of Acute Variceal Bleed in Patients with Liver Cirrhosis, 21(3), pp. 191–196.

Ferreira, E. A. et al. 2010. Potent hepatoprotective effect in CCl 4 -induced hepatic injury in mice of phloroacetophenone, 2820. doi: 10.3402/ljm.v5i0.4891.

Bhara, M. 2015. Pengaruh pemberian kopi dosis bertingkat per oral 30 hari terhadap gambaran histologi hepar tikus wistar (Doctoral dissertation, Medical Faculty).

Kamimura, D; Ishihara, K; Hirano, T. 2003. IL-6 signal transduction and its physiological roles: the orchestration model. Reviews of Physiology, Pharmacology 149: 1-38.

Khotimah, C. 2015. Pengaruh Pemberian Propolis Terhadap Jumlah Dan Hitung Jenis Leukosit Darah Mencit (Mus Musculus) Jantan. Journal Of Basic Medicine Veterinary. Vol.4 No.1, Hal. 28-34.

Kusdalinah, Andrew J, N. W. 2014. Ekstrak Buah Naga Daging Merah (Hylocereus polyrhizus) terhadap Berat Badan, Indeks Fagositosis Makrofag dan Produksi Nitrit Oksida Makrofag (Studi pada Mencit yang Diinfeksi Salmonella thyphimurium), Jurnal Gizi Indonesia, 2(2), pp. 73–77.

Kementerian Kesehatan RI. 2014. Situasi Dan Analisis Hepatitis Pusat Data Dan Informasi. Jakarta.

Kementerian Kesehatan RI. 2010. Riset Kesehatan Dasar. Jakarta.

Lengkong, A. B. 2013. Gambaran Histopatologik Hati Tikus Wistar Yang Diinduksi CCL4 dan diberi Air Rebusan Tanaman Cakar Ayam (Selaginella Doederleinii Hieron). Jurnal e-Biomedik, 1(2).

Linawati. 2018. Purple Cabbage Extract Cream Effect on Erythema Score of Male Wistar Rats Back Skin Exposed to UV-B Radiation. Biomedical and Pharmacology Journal, 11(1), 343-351.

Maulina, Meutia. 2013. Pengaruh Pemberian Xanthone Terhadap Gambaran Histopatologi Sel Hepar Tikus Putih Jantan (Rattus Norvegicus) Yang Diinduksi Karbon Tetraklorida (CCl4) Penelitian Eksperimental Laboratorik. Diss. Universitas Airlangga.

Krisnansari, D., Sulistyo, H., & Ati, V. R. B. 2014. Efek Propolis Terhadap Fungsi dan Perlemakan Hati Tikus Putih (Rattus norvegicus) Model Hiperkolesterolemia. Nutrition and Food Research, 37(1), 77-85.

Nunekpeku, W. et al. 2018. Screening for total antioxidant activity, flavonoids and phenolics variability in forty-five accessions of roselle ( Hibiscus sabdariffa L .), 17(22), pp. 694–699. doi: 10.5897/AJB2017.16286.

Park, C. M. et al. 2010. Amelioration of Oxidative Stress by Dandelion Extract through CYP2E1 Suppression against Acute Liver Injury Induced by Carbon Tetrachloride in Sprague-Dawley Rats’, 1353), pp. 1347–1353.

Perez, A. B. et al. 2010. Tumor necrosis factor-alpha, transforming growth factor-β1, and interleukin-10 gene polymorphisms: Implication in protection or susceptibility to dengue hemorrhagic fever’, Human Immunology. Elsevier Inc., 71(11), pp. 1135–1140. doi: 10.1016/j.humimm.2010.08.004.

Prasetyo, D. H., Suparyanti, E. L. and Guntur, A. H. 2011. Ekstrak Etanol Propolis Isolat Menurunkan Derajat Inflamasi dan Kadar Malondialdehid pada Serum Tikus Model Sepsis Ethanol extract of Propolis Reduces the Level of Inflammation and Serum Malondialdehyde in Sepsis Rats Model, 45(36), pp. 161–166.

Rohmatin, A. R., Susetyarini, E. and Hadi, S. 2012. Kerusakan Sel Hepar Tikus Putih Jantan ( Rattus norvegicus ) yang di Induksi Karbon Tetraklorida ( CCl 4 ) setelah Diberi Ekstrak Etanol Bawang Dayak ( Eleutherine palmifolia Merr .) The Damage of Hepar Cells of White Male Mice ( Rattus norvegicus ) which, pp. 942–947.

Soroy, L. et al. 2014. The effect of a unique propolis compound (propoelixTM) on clinical outcomes in patients with dengue hemorrhagic fever, Infection and Drug Resistance, 7, pp. 323–329. doi: 10.2147/IDR.S71505.

Tendean, L. 2016. Perbandingan kualitas spermatozoa tikus wistar (rattus norvegicus) yang diberi paparan asap rokok dengan asap rokok elektronik Miseal Tooy Kandidat skripsi Fakultas KedokteranUniversitas Sam Ratulangi Manado Peraturan Pemerintah Republik Indonesia, 4.

Timur, J. et al. 2017. Efek Pemberian Propolis terhadap Gambaran Histopatologi Hepar Tikus Putih yang diberi Parasetamol Of White Rats Given Paracetamol, 9(1), pp. 87–93. doi: 10.21531/bulvet.2017.9.1.87.

WHO. 2017. Study on Assessment of Renal Function in Chronic Liver Disease (2015), pp. 9–12. doi: 10.7860/JCDR/2015/11423.5658.




DOI: http://dx.doi.org/10.30659/sainsmed.v11i1.4882

Article Metrics

Metrics Loading ...

Metrics powered by PLOS ALM

Refbacks



Indexed by:

 

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.
Based on a work at http://jurnal.unissula.ac.id/index.php/sainsmedika.

site
stats View My Stats.