The Difference between the Effect of Green Tea Cream and Tocopherol on Decreasing Level of Tyrosinase Enzyme and Amount of Melanin in Rattus norvegicus Exposed to UVB Rays

Anwar Riyanto, Taufiqurrachman Nasihun, Titiek Sumarawati

Abstract


Introduction: Green tea and tocopherol are potent antioxidants used to treat melasma. However, whether green tea or tocopherol is superior remains unclear. Objective: To compare the effectiveness between green tea and tocopherol in decreasing the amount of melanin and tyrosinase level in wistar rats exposed to UVB rays.

Methods: The experimental study took 30 male Wistar rats randomly and divided them into 3 groups. The control group (C-G) was given basic topical cream, GT-G was given green tea topical cream and TC-G was given tocopherol topical cream. All rats were exposed to UVB every Monday, Wednesday and Friday for 4 weeks, whereas topical creams were smeared every day. Topical creams smearing on the same day with UVB exposure was performed 20 minutes before exposure and 4 hours after UVB exposure. The doses of UVB were 50 mJ/cm2 in first week, 70mJ/cm2 in the second week and 80mJ/cm2 in the third and fourth weeks. The amount of melanin was measured using pixel method and the tyrosinase level was measured using ELISA.

Results: Anova analysis indicates that the amount of melanin and tyrosinase level are significantly different between groups, p<0.05. Post Hoc LSD analysis indicates that the amount of melanin in GT-G and TC-G are significantly lower than that of C-G, p<0.05. The amount of melanin in GT-G is lower than that of TC-G, p<0.05. The tyrosinase level in GT-G is significantly lower than that of C-G and TC-G, p<0.05. Meanwhile, the tyrosinase level in TC-G is lower than that of C-G but insignificantly, p>0.05. 

Conclusion: Green tea topical treatment is significantly capable of decreasing the amount of melanin and tyrosinase level better than tocopherol.


Keywords


Green tea; tocopherol; amount of melanin; tyrosinase level

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References


Albakry, N. A., Palm, M., Board, O., Amiyati, Y., Palm, M., Board, O., Aldrin, Z., Palm, M., & Board, O. (2018). The Mechanism and Potential of Palm Vitamin E as a Skin-lightening Agent. June.

Badreshia-Bansal S, D. Z. (2007). Insight into skin lightening cosmeceuticals for women of color. Journal of Drugs in Dermatology : JDD, 6(1), 32–39.

Chai, W.-M., Yan, C.-L., Chen, X.-X., Gao, Y.-S., Tian, L., Feng, H.-L., Chen, Q.-X., & Shi, Y. (2014). Isolation and Purification of Condensed Tannins from Flamboyant Tree and Their Antioxidant and Antityrosinase Activities. Applied Biochemistry and Biotechnology, 173(1), 179–192. https://doi.org/10.1007/s12010-014-0828-z

Chen, Q. H., Qin, F., Sumpio, B. E., Cordova, A. C., & Berke-Schlessel, D. W. (2006). Green Tea, the “Asian Paradox,” and Cardiovascular Disease. Journal of the American College of Surgeons, 202(5), 813–825. https://doi.org/10.1016/j.jamcollsurg.2006.01.018

Cichorek, M., Wachulska, M., Stasiewicz, A., & Tymińska, A. (2013). Skin melanocytes: Biology and development. Postepy Dermatologii i Alergologii, 30(1), 30–41. https://doi.org/10.5114/pdia.2013.33376

D’Orazio, J., Jarrett, S., Amaro-Ortiz, A., & Scott, T. (2013). UV radiation and the skin. International Journal of Molecular Sciences, 14(6), 12222–12248. https://doi.org/10.3390/ijms140612222

Deny, F., & Hakim, Z. (2006). Penggunaan Vitamin E Dan Vitamin C Topikal. Majalah Kedokteran Andalas,Padang, (2) 30(2).

Dissanayake, C. Y., Moon, H. H., Yang, K. M., Lee, Y., & Han, C. H. (2018). The effects of green tea (Camellia sinensis) flower extract on melanin synthesis in B16-F10 melanoma cells. Korean Journal of Veterinary Research, 58(2), 66–72. https://doi.org/10.14405/kjvr.2018.58.2.65

Gramza, A., & Korczak, J. (2005). Tea constituents ( Camellia sinensis L .) as antioxidants in lipid systems. Food Science and Technology (Campinas),Poznan Poland University, 16, 351–358. https://doi.org/10.1016/j.tifs.2005.02.004

Hadiyati, P., Sebero, H., & Apriliana, E. (2014). Kualitas Hidup pada Pasien Melasma di RSUD Dr. H. Abdul Moeloek Lampung. Jurnal Kedokteran Universitas Lampung, 3(5), 130–138.

Jusuf, N. K. (2017). Pattern of pigmentation disorder in Cosmetic Dermatology Clinic H. Adam Malik General Hospital, Medan, 2012 -2015. J Gen Proced Dermatol Venereol Indones, 2(1), 1–6.

Kamei, Y., Otsuka, Y., & Abe, K. (2009). Comparison of the inhibitory effects of vitamin E analogues on melanogenesis in mouse B16 melanoma cells. Cytotechnology, 59(3), 183–190. https://doi.org/10.1007/s10616-009-9207-y

Katiyar, S. K., Afaq, F., & Perez, A. (2001). Green tea polyphenol (–) -epigallocatechin-3-gallate treatment of human skin inhibits ultraviolet radiation-induced oxidative stress The use of naturally occurring botanicals with substantial antioxidant activity to afford protection to human skin against. Change, 22(2), 287–294.

Keen, M., & Hassan, I. (2016). Vitamin E in dermatology. Indian Dermatology Online Journal, 7(4), 311. https://doi.org/10.4103/2229-5178.185494

Kim, Y. C., Choi, S. Y., & Park, E. Y. (2015). Anti-melanogenic effects of black, green, and white tea extracts on immortalized melanocytes. Journal of Veterinary Science, 16(2), 135–143. https://doi.org/10.4142/jvs.2015.16.2.135

Kim, Y. J., & Uyama, H. (2005). Tyrosinase inhibitors from natural and synthetic sources: Structure, inhibition mechanism and perspective for the future. Cellular and Molecular Life Sciences, 62(15), 1707–1723. https://doi.org/10.1007/s00018-005-5054-y

Lim, J. Y., Kim, O. K., Lee, J., Lee, M. J., Kang, N., & Hwang, J. K. (2014). Protective effect of the standardized green tea seed extract on UVB-induced skin photoaging in hairless mice.

Nutrition Research and Practice, 8(4), 398–403. httchen, B. E. (2016). Uji Aktivitas Antioksidan dan Penghambatan Tirosinase serta Uji Manfaat Gel Ekstrak Kulit Batang Taya ( Nauclea subdita ) terhadap Kulit. Jurnal Kefarmasian Indonesia, 6(2), 98–107. https://doi.org/10.22435/jki.v6i2.6224.98-107

Miot, H. A., Brianezi, G., Tamega, A. de A., & Miot, L. D. B. (2012). Techniques of digital image analysis for histological quantification of melanin. Anais Brasileiros de Dermatologia, 87(4), 608–611. https://doi.org/10.1590/S0365-05962012000400014

Nichols, J. A., & Katiyar, S. K. (2011). Polyphenols: skin photoprotection and inhibition of photocarcinogenesis. Mini Reviews in Medicinal Chemistry, 11(14), 1200–1215. https://doi.org/10.1007/s00403-009-1001-3.Skin

, R. M. M. De. (2012). Quantification of catechins and caffeine from green tea (Camellia sinensis) infusions, extract, and ready-to-drink beverages. Food Science and Technology (Campinas), 32(1), 163–166. https://doi.org/10.1590/S0101-20612012005000009

Rahman, I., Biswas, S. K., & Kirkham, P. A. (2006). Regulation of inflammation and redox signaling by dietary polyphenols. Biochemical Pharmacology, 72(11), 1439–1452. https://doi.org/10.1016/j.bcp.2006.07.004

Saral, Y., Uyar, B., Ayar, A., & Naziroglu, M. (2002). Protective effects of topical alpha-tocopherol acetate on UVB irradiation in guinea pigs: Importance of free radicals. Physiological Research, 51(3), 285–290.

Tripathi, R. K., Giebel, L. B., Strunk, K. M., & Spritz, R. A. (2018). CE N E EXPRESSION is associated with temperature-sensitive enzymatic activity. Cichago Medical School, 1(2), 103–110.

Uwabara, Y. K., Atanabe, T. W., Asuoka, S. Y., Ukui, K. F., & Akata, J. T. (2006). Topical Application of g -Tocopherol Derivative Prevents UV-Induced Skin Pigmentation. Biol. Pharm. Bull., 29(June), 1175–1179. https://doi.org/10.1248/bpb.29.1175

Woolery-Lloyd, H., & Kammer, J. N. (2011). Treatment of Hyperpigmentation. Seminars in Cutaneous Medicine and Surgery, 30(3), 171–175. https://doi.org/10.1016/j.sder.2011.06.004

Yamaguchi, Y., & Hearing, V. J. (2009). Physiological factors that regulate skin pigmentation. BioFactors, 35(2), 193–199. https://doi.org/10.1002/biof.29




DOI: http://dx.doi.org/10.30659/sainsmed.v11i1.4347

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