Effect of Celery Extract Administration on 8-OHdG and Number of Foam Cell in Wistar Strain Rats With A High-Fat Diet

Agnes Budiarto*  -  Master of Biomedical Sciences, Faculty of Medicine Sultan Agung Islamic University, Semarang, Indonesia
Taufiqurrachman Nasihun  -  Master of Biomedical Sciences, Faculty of Medicine Sultan Agung Islamic University, Semarang, Indonesia
Atina Hussaana  -  Master of Biomedical Sciences, Faculty of Medicine Sultan Agung Islamic University, Semarang, Indonesia

(*) Corresponding Author

BACKGROUND: A high fat diet, accompanied by high frequency eating, results in hyperlipidemia. One prevention for hyperlipidemia is administration of celery extract.

OBJECTIVE: to explore the effect of celery extract on the level of 8-OHdG (8 -Hydroxy-2’-Deoxy Guanosine) and the number of foam cell.


METHODS: Experimental studies with post-test only control group design used Wistar rats, administered with adrenaline and high-fat diet for
hyperlipidemic induction.Total of 35 rats were divided into 5 groups: negative controls (Neg-G); positive control treated with simvastatin (Nor-G); ES25-G,ES50-G, and ES75-G group were treated with 25 mg/200 g BW, 50 mg/200 g BW, and 75 mg/200 g BW celery extract, respectively. The treatment was given for 30 days. The 8-OHdG level was determined by ELISA, whereas the number of foam cells was determined by histopathologic preparations with HE staining. Data analysis was conducted by Kruskal-Wallis and Mann-Whitney.


RESULTS: Mann-Whitney analysis showed levels of 8-OHdG in ES-25-G 12.13±0.21, ES-50-G 7.23±0.25, ES-75-G 4.41±0.45 significantly lower
than the Nor-G 14.30±0.66, respectively p<0.001. The number of foam cells in the ES-25-G was 7.57±0.53, ES-50-G 6.57±0.79, ES-75-G 3.43±0.53,significantly lower than the Neg-G 13.57±1.27, respectively p<0.001.

CONCLUSION: Celery extract capable of decreasing levels of 8-OHdG and the number of foam cells in Wistar rats induced with adrenaline and high-fat
diet.

Keywords: celery;8-OHdG;foam cell

Sains Medika: Jurnal Kedokteran dan Kesehatan
is published by Faculty of Medicine Universitas Islam Sultan Agung, Indonesia in association with FOKI (FORUM KEDOKTERAN ISLAM INDONESIA).

Contact: Jl. Raya Kaligawe Km.4, PO BOX 1054/SM Semarang 50112, Indonesia
Phone+62 8122-9933-369
Website: https://fkunissula.ac.id
Email: sainsmedika@unissula.ac.id

ISSN: 2339-093X (Online) | 2085-1545 (Print)
DOI : 10.30659/sainsmed

This work is licensed under a Creative Commons Attribution 4.0 International License

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