The effect of Gandarusa Leaf extract (Justicia gendarussa Burm F.) Administration on Estradiol Hormone Level and the Amount of Antral Ovarium Follicle on Female Mice

ni'mah hidayatullaili

Abstract


 

Background: Physiologically, woman experiencing aging characterized by menopause. Gandarusa leaf contains isoflavon, a phytoesterogen expected to be used as esterogen replacement therapy. Objective: the aim of this research is to investigate the administrative effect of gandarusa leaf extract on the increase of estradiol hormone level and the number of antral ovarium follicle of female mice.

Methods: This is experimental study with posttest only control group research design. Samples are 24 balb-c female mice, aged 16-17 months, weighed 19-35 grams, divided into 4 groups. Control group (Ctrl-G) were given 0.48 ml aquadest; group JB10-G, JB20-G, and JB30-G, each were given gandarusa leaf extract with concentration 10%, 20%, dan 30% in volume 0.48 ml orally twice a day. After treatment, dissection were conducted to make ovarium histology preparation with Hematoxylin Ehrlich-Eosin staining, observation were conducted using microscope.

Results: Post hoc analysis indicated that the estradiol level and the number of antral ovarium follicle on administration of gandarusa leaf extract with concentration 10%, 20%, dan 30% are significantly higher, p < 0.05. The increase of estradiol level with extract concentration of 10% are significantly negatively correlated with the number of antral ovarium follicles p<0.05.

Conclusion: The administration of gandarusa leaf extract increase the estradiol hormone level and the number of antral ovarium follicle in female mice.

 

Keywords : gandarusa leaves extract, estradiol endogenous, ovary histology




DOI: http://dx.doi.org/10.26532/sainsmed.v9i2.2099

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Copyright (c) 2019 Sains Medika

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.
Based on a work at http://jurnal.unissula.ac.id/index.php/sainsmedika.

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