Development of Anti Acne Cream (w/o/w Multiple Emulsion) Containing Green Tea Leaf Waste

Naniek Widyaningrum*  -  Pharmacy program, Medicine Faculty, Sultan Agung Islamic University, Indonesia

(*) Corresponding Author

Background: To date, green tea leaf waste is not well utilized, the waste is remain considered as a waste. Previous studies found that green tea leaf waste still have a fairly high EGCG content. EGCG has benefits as an antibacterial. Utilization of green tea leaf waste started from formulation until the diversification of multiple emulsion w/o/w products has not been reported.

Objective: To develop anti acne cream (W/O/W) multiple emulsion containing green tea leaf waste and evaluate its antibacterial activity against acne-inducing bacteria of Propionibacterium acnes (P.acnes).


Methods: Phase one of this research was green tea leaves was extracted by infundation and fractionation method using ethyl acetate and prepared in different concentration (1% to 6%). The second stage was ethyl acetate fraction of green tea leaf extract was tested for its antibacterial activity against P.acnes. Third stage was tested physical properties of formulation with w/o/w multiple emulsion.


Results: The optimal antibacterial activity of ethyl acetate fraction of green tea leaf extract against P.acne was at the concentration of 6%, with inhibition zone of 32.6 mm ±0.57. The formula of w/o/w multiple emulsion loaded with green tea leaves waste of 6% active substance demonstrated a good physical properties which can spread 90.4 cm2 ± 0.03, pH 5.00 ± 0.02 and microscopic analysis showed multiple emulsion w/o/w.

Conclusion: The concentration of 6% green tea leaf waste formulated in multiple emulsion had a good physical and antibacterial activity for a referred standard. It is necessary to test the stability of multiple emulsion formula.

Keywords : Multiple emulsion W/O/W;green tea leaf residue;Anti acne;P.acnes

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Sains Medika : Jurnal Kedokteran dan Kesehatan
is published by Fakultas Kedokteran Universitas Islam Sultan Agung Semarang, Indonesia.
Jl. Raya Kaligawe Km.4, PO BOX 1054/SM Semarang 50112
Website: https://fkunissula.ac.id/
Email: sainsmedika@unissula.ac.id

ISSN: 2339-093X (Online) | 2085-1545 (Print)
DOI : 10.30659/sainsmed

This work is licensed under a [note] - Creative Commons Attribution 4.0 International License

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