DEVELOPMENT OF MULTIPLE EMULSION CREAM SUPLY FORMULATION W/O/W ON GREEN TEA LEAF RESIDUE AS ANTI ACNE

Naniek Widyaningrum

Abstract


ABSTRACT

Background:
Residue of green tea leaves is not well utilized until now, the residue is still considered as a waste. Previous studies found that green tea leaf residue still have a fairly high EGCG content. EGCG has benefits as an antibacterial. Utilization of waste of green tea residue started from formulation until the diversification of multiple emulsion w/o/w products has not been reported yet. The purpose of this study is to obtain the dosage and waste formula of green tea leaves in the preparation of multiple emulsion w/o/w cream effective in Propionibacterium acnes (P.acnes) causing bacteria.
Method:
Phase one of this research was green tea leaf waste extraction done by infundation and fractionation method using ethyl acetate. The second stage was ethyl acetate fraction of green tea leaf extract tested its antibacterial activity on P.acnes bacteria with concentration of 1% to 6%, third stage was formulation with multiple emulsion w/o/w tested its physical properties.

Result:
The results of this research concentration of ethyl acetate fraction of green tea leaf extract of 6% was the optimum concentration in inhibiting the growth of P.acnes bacteria with inhibition zone 32,6 ± 0,57. The formula of preparation of multiple emulsion w/o/w with green tea leaves of 6% active substance has good physical properties which can spread 90.4 cm2 ± 0.03, pH 5.00 ± 0.02 and microscopic analysis showed multiple emulsion w/o/w. The concentration of 6% green tea leaf residue in the formula of multiple emulsion has a good physical and antibacterial activity so that it can be used as reference of formulation. It is necessary to test the stability of multiple emulsion formula.

 


Keywords


Multiple emulsion W/O/W, green tea leaf residue, Anti acne, P.acnes

Full Text:

PDF

References


Bashir, S., B.M. Khan, M. Babar, S. Andleeb, M. Hafeez, S. Ali and M.F. Khan, 2014. Assessment of bioautography and spot screening of TLC of green tea (Camellia Sinensis L.) plant extracts as antibacterial and antioxidant agents. Indian J. Pharmaceut. Sci., 76: 364-370.

Bek-Thomsen, M., Lomholt, H. B., dan Kilian, M., 2008. Acne is Not Associated with Yet-Uncultured Bacteria. J. Clin. Microbiol., 46(10), 3355–3360.

Mahmood, T., Akhtar, N., dan Manickam, S., 2014. Interfacial film stabilized W/O/W nano multiple emulsions loaded with green tea and lotus extracts: systematic characterization of physicochemical properties and shelf-storage stability. J of Nanobiotech, 12: 1–17.

Niyomkam, P., Kaewbumrung, S., Kaewnpparat, S., dan Panichayupakaranant, P., 2010. Antibacterial activity of Thai herbal extracts on acne involved microorganism. Pharm. Biol., 48(4), 375–380.

Stratton, S. P., Bangert, J. L., Alberts, D. S., dan Dorr, R. T., 2000. Dermal toxicity of topical (-)epigallocatechin-3-gallate in BALB/c and SKH1 mice. Cancer Lett., 158(1), 47–52.

Widyaningrum, N., Murrukmihadi, M., dan Ekawati, S.K., 2012. Pengaruh Konsentrasi Ekstrak Etanolik Daun Teh Hijau (Camellia sinesis L.) dalam Sediaan Krim terhadap Sifat Fisik dan Aktivitas Antibakteri. Jurnal Sains Medika, 4: 147–156.

Widyaningrum N, Fuholi A, Sudarsono, Setyowati EP, 2015a, The Stability of Epigallocatechin gallate (EGCG) from Green Tea (Camellia sinensis) and its Antibacterial Activity against Staphylococcus epidermidis ATCC 35984 and Propionibacterium acnes ATCC 6919, Asian. J. of Biol. Sci, 8(2), 93-101.

Widyaningrum N, Fuholi A, Sudarsono, Setyowati EP, 2015b, Buffer And Emulsifier Optimization In Cream With its Antibacterial Activity And Sensitivity, Int. J. of Pharm. Sci and Research, 6(12), 5253-5258.

Widyaningrum N, Fuholi A, Sudarsono, Setyowati EP, 2015c, The Effect of Altitude Against Total Phenolic and Epigallocatechin Gallate (EGCG) Content in Green Tea Leaves, International Converence on science and science education.

Widyaningrum, N., Badie’ah., Lestari, S., 2017, Antibacterial activity of the dregs of green tea leaves (Camellia sinensis L) on S.epidermidis as causes of acne, 2nd international conference on science and science education (IConSSE).




DOI: http://dx.doi.org/10.26532/sainsmed.v8i2.2030

Article Metrics

Metrics Loading ...

Metrics powered by PLOS ALM

Refbacks

  • There are currently no refbacks.


Copyright (c) 2018 Sains Medika

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.
Based on a work at http://jurnal.unissula.ac.id/index.php/sainsmedika.

site
stats View My Stats.