Naniek Widyaningrum



Residue of green tea leaves is not well utilized until now, the residue is still considered as a waste. Previous studies found that green tea leaf residue still have a fairly high EGCG content. EGCG has benefits as an antibacterial. Utilization of waste of green tea residue started from formulation until the diversification of multiple emulsion w/o/w products has not been reported yet. The purpose of this study is to obtain the dosage and waste formula of green tea leaves in the preparation of multiple emulsion w/o/w cream effective in Propionibacterium acnes (P.acnes) causing bacteria.
Phase one of this research was green tea leaf waste extraction done by infundation and fractionation method using ethyl acetate. The second stage was ethyl acetate fraction of green tea leaf extract tested its antibacterial activity on P.acnes bacteria with concentration of 1% to 6%, third stage was formulation with multiple emulsion w/o/w tested its physical properties.

The results of this research concentration of ethyl acetate fraction of green tea leaf extract of 6% was the optimum concentration in inhibiting the growth of P.acnes bacteria with inhibition zone 32,6 ± 0,57. The formula of preparation of multiple emulsion w/o/w with green tea leaves of 6% active substance has good physical properties which can spread 90.4 cm2 ± 0.03, pH 5.00 ± 0.02 and microscopic analysis showed multiple emulsion w/o/w. The concentration of 6% green tea leaf residue in the formula of multiple emulsion has a good physical and antibacterial activity so that it can be used as reference of formulation. It is necessary to test the stability of multiple emulsion formula.



Multiple emulsion W/O/W, green tea leaf residue, Anti acne, P.acnes

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