Administration of Mung Bean Extract (Phaseolus radiatus) in Increasing Hb and Ferritin Level and Decreasing Malondyaldehide (MDA) Level in Anaemic Rats

Heni Wijayanti*  -  Master of Biomedical Sciences Sultan Agung Islamic University Semarang Indonesia, Indonesia
Taufiqurrachman Nasihun  -  Master of Biomedical Sciences Sultan Agung Islamic University Semarang Indonesia, Indonesia
Atina Hussaana  -  Master of Biomedical Sciences Sultan Agung Islamic University Semarang Indonesia, Indonesia

(*) Corresponding Author

BACKGROUND: Mung bean, not only contains protein, carbohydrates, and fats, but also contains iron and vitamin C which are proven to overcome anemia in pregnant woman. High iron levels are potential to increase ROS production through fenton reaction.

OBJECTIVE: to prove the administration of mungbean can increase Hb and ferritin levels and decreasing malondihaldehyde (MDA) in anaemic rats.


METHODS: This research uses Post Test Only Control Group Design. A total of 25 anaemic rats were divided into 5 groups: normal group (Nor-G);negative control group (Neg-G), were given low Fe diet; and treatment group with low Fe and mung bean extract at dose of 0,18g/200g/days (GP-0,18), 0,36g/200g/days (GP-0.36), dan 0,72g/200g/days (GP-0.72). Low Fe diet and mung bean extract were administered for 14 days. Hb levels were measured using Sahli method, ferritine level using Immulite Ferritine Kit, and MDA were measured using TBA.


RESULTS: Compare to Nor-G and Neg-G, levels of ferritin and Hb on groups with mung bean extract 0,18g (75.56), 0,36g (90.98) and 0,72g (95.87) were significantly higher (p < 0.05). While MDA levels on groups with mung bean 0,18g (4.646), 0,36g (3.396) and 0,72g (1.92) were significantly lower than on Nor-G and Neg-G groups (p < 0.05).


CONCLUSION: The administration of mung bean extract can increase Hb and ferritin, also lower MDA level on anaemic rats.

Keywords : Mung bean extract;Ferritin level and MDA level

  1. AlDallal S. Iron Deficiency Anemia: A Short Review. J Immunooncol. 2016;2: 1-6
  2. Andrews NC. Forging a field: The golden age of iron biology. Blood. 2008; 112: 219-230.
  3. Ajioka RS, Phillips JD, Kushner JP. Biosynthesis of heme in mammals. Biochimica et Biophysica Acta. 2006; 1763: 723–736
  4. Agrawal S, Misra R, Aggarwal A. Anemia in rheumatoid arthritis: high prevalence of iron-deficiency anemia in Indian patients. Rheumatol Int (2006) 26: 1091–1095. DOI 10.1007/s00296-006-01334.
  5. Barbusinski K. Fenton Reaction-Controversy Concerning The Chemistry. ECOLOGICAL CHEMISTRY AND ENGINEERING S. 2009; 16(3): 347-358
  6. Burke RM, Leon JS, Suchdev PS. Identification, Prevention and Treatment of Iron Deficiency during the First 1000 Days. Nutrients. 2014: 6: 4093-4114; doi:10.3390/nu6104093
  7. Fisher AEO. Iron supplements; the quick fix with longterm consequences. Nutr j. 2007; 3(2):1-5
  8. Ganesan K, Xu B, A critical review on phytochemical profile and health promoting effects of mung bean (Vigna radiata), Food Sci. Hum. Wellness. 2017; 11: 1-22.
  9. Helty. 2010. Pengaruh kacang hijau terhadap kadar hemoglobin dan jumlah sel darah dalam konteks asuhan keperawatan pasien kanker dengan kemoterapi (tesis). 2010; Http://www.jurnal.lib.ui.ac.id dikutip 7 Maret 2016.
  10. Iswandari R. Studi Kandungan Isoflavon pada Kacang Hijau, Tempe Kacang Hijau, dan Bubur Kacang Hijau. Karya Tulis Ilmiah. Semarang: Fakultas Kedokteran Universitas Diponegoro; 2008.
  11. Kementerian Kesehatan RI. Riset Kesehatan Dasar (Riskesdas) 2013. Badan Penelitian dan Pengembangan Kesehatan Kementerian Kesehatan RI. Jakarta: 2013.
  12. Mahmud MK, Zulfianto NA, Hermana, Apriyantono RR, Ngadiarti I, Hartati B, et al. Dalam Mahmud MK, Zulfianto NA, editors. Tabel Komposisi Pangan Indonesia (TKPI). Jakarta: Elex Media Komputindo; 2009. 10-17.
  13. Maulina N, Sitepu IP. Pengaruh Pemberian Kacang Hijau (Phaseolus radiatus) Terhadap Peningkatan Kadar Hemoglobin Tikus Putih (Rattus norvegicus) Jantan Galur Wistar. JPKim. 2015; 7 (2): 57-60.
  14. Moriyama M, Oba K. Comparative Study on the vitamin C content of the food Legumee seeds.J Nutr Sci Vitaminol. 2008; 54: 1-6
  15. Nasihun,T and Widayati, E. Administration of Purwoceng (Pimpinellaalpina Molk) Improves Oxidative Stress Biomarker Following UVC Irradiation in Sprague-Dawley Male Rats.JOURNAL OF NATURAL REMEDIES. 2016;15(3): 82-91. DOI: 10.18311/jnr/2016/7684
  16. Novidiyanto N, Farmawati A, Lestari LA. Pengaruh pemberian kecambah kacang hijau (Phaseolus radiatus (L.)) terhadap kadar malondealdehid (MDA) plasma dan jaringan hati tikus Sprague Dawley yang diberi pakan lemak tinggi. Jurnal Gizi Klinik Indonesia. 2016; 13(2): 82-89
  17. Patimah, St; Pola konsumsi ibu hamil dan hubungannya dengan kejadian anemia defisiensi besi. Jakarta, Badan penelitian sains dan teknologi. 2009; 46-52
  18. Purushothaman V, Amirthaveni M, Samson CS Tsou SCS, Shanmugasundaram S. Supplementing iron bioavailability enhanced mung bean. Asia Pac J Clin Nutr. 2008;17:99-102
  19. Sudikno, Sandjaja. Prevalensi dan Faktor Risiko Anemia Pada Wanita Usia Subur di Rumah Tangga Miskin di Kabupaten Tasikmalaya dan Ciamis, Provinsi Jawa Barat. Jurnal Kesehatan Reproduksi. 2016; 7( 2): 71-82
  20. Siah CW, Ombiga J, Adams LA, Trinder D, Olynyk JK. Normal iron metabolism and the pathophysiology of iron overload disorders. Clin Biochem. 2006; 27: 5-16
  21. Wimbley JTD, Graham D. Diagnosis and management of iron deficiency anemia in the 21st century. Therapeutic Advances in Gastroentesrology. 2011; 4: 177-184.
  22. Yusuf. Pemanfaatan Kacang Hijau Sebagai Pangan Fungsional Mendukung Diversifikasi Pangan di Nusa Tenggara Timur. Prosiding Seminar Hasil Penelitian Tanaman Aneka Kacang Dan
  23. Umbi. 2014; 741-746
  24. Zhan S, Ho SC. Meta-analysis of the effect of soy protein containing isoflavones on the lipid. Am j clin nutr. 2007; 81: 397- 408

Copyright (c) 2018 Sains Medika

Sains Medika : Jurnal Kedokteran dan Kesehatan
is published by Fakultas Kedokteran Universitas Islam Sultan Agung Semarang, Indonesia.
Jl. Raya Kaligawe Km.4, PO BOX 1054/SM Semarang 50112
Website: https://fkunissula.ac.id/
Email: sainsmedika@unissula.ac.id

ISSN: 2339-093X (Online) | 2085-1545 (Print)
DOI : 10.30659/sainsmed

This work is licensed under a [note] - Creative Commons Attribution 4.0 International License

Get a feed by atom here, RRS2 here and OAI Links here

apps