Red Ginger (Zingiber officinale var. Amarum) Infusion Increases Concentration of Luteinizing Hormone and Testosterone in Male Rats

Alida Nihayah, Taufiqurrahman Nasihun, Israhnanto Isradji


Introduction: Herbal remedies have been developed as a complementary therapy to increase sex hormones. The administration of the ethanolic extract of red ginger (Zingiber officinale var. Amarum) has been shown to increase spermatogenic cells, motility and morphology of sperm in mice. However, the effect of red ginger infusion on the level of testosterone and luteinizing hormone (LH) has not been done. Objective: to examine the effect of red ginger infusion on LH and testosterone levels of male Wistar rats.

Methods: The study was designed as an experimental post-test only control group design. Twenty four male Wistar rats of 200-250 g body weight aged + 3-4 months were divided into four groups. a control group (G-1) was given distilled water 2 ml/day. Group 2 (G-2), Group 3 (G-3), and group 4 (G-4) were treated with 10%, 20%, and 40% infusion by dissolving 2 ml of ginger in distilled water. On day 15, blood samples were taken and assayed for LH and testosterone.

Results: The one-way ANOVA test showed difference in levels of LH and testosterone between groups (p <0.001). Post Hoc LSD test results showed that the levels of LH of G-1 (0350) was significantly lower than that of G-2 (1082), G-3 (1 253), and G-4 (0923), p <0.005. While the levels of testosterone in the G-1 (0.2718) significantly lower than the G-2 (0.7797), G-3 (0.8285), and the G-4 (0.9142) (p <0.005).

Conclusion: red ginger infusion for 14 days can increase the levels of LH and testosterone in male rats.


LH; Testosterone; infusion of Red Ginger

Full Text:



Erstad, S and Havens, L. 2006. Gonadotropin Treatment for Infertility. Obstetric and Gynecology. HealthWise.

Jones, T.,H. 2008. Testosteron deficiency:an overview.In Jones,T.,H ed. Testosteron deficiency in man. 1st ed. Oxford University Press. 1: 1-8.

Kaspul., 2007. Kadar kolesterol tikus putih setelah mengkonsumsi buah terong pukak solano torvum S W . Program studi pendidikan biologi fakultas keguruan dan ilmu pendidikan lambung Kalimantan selatan. Biosenteae. Vol. 4 (1): 1 -8. (Indonesia).

Mahendra, T. 2009. Pengaruh Pemberian Ekstrak Rimpang Jahe Merah (Zingiber ofiicinale Roscoe var. Rubrum) Terhadap Motilitas dan Konsentrasi Spermatozoa Tikus Putih (Rattus novergicus) Jantan. Artikel Ilmiah Universitas Airlangga. Surabaya.

Maisuri, RA. 2013. Pengaruh Pemberian Ekstrak Jahe Merah (Zingiber Officinale Roxb. Var Rubrum) dan Zinc (Zn) Terhadap Jumlah, Motilitas, dan Morfologi Spermatozoa pada Tikus Putih (Rattus norvegicus) Jantan Dewasa Strain Sprague Dawley. Medical Journal of Lampung University (2).

Misro M. M,. Ganguly A. R. Das P. 2008. Is Testosterone Essential for Maintenance of Normal Morphology in Immature Rat Leydig Cells. International Journal of Andrology. Volume 16 Issue 3, Pages 221 – 6.

Mohammad H, 2010. Pengaruh Pemberian Ekstrak Jahe Merah (Zingiber officinaqle Rosc) Terhadap Kualitas Spermatozoa Tikus (Rattus norvegicus) Yang Terpapar Allethrin. Website (Indonesia).

Pangkahila, W. 2007. Anti-Aging Medicine : Peranan Hormon. Jakarta: PB Kompas. Bab 4: 70-80. (Indonesia).

Papadopoulos V, Miller WL 2012. Role of mitochondria in steroidogenesis. Best Pract Res Clin Endocrinol Metab. 26:771-90.

Rudy S, 2013, Khasiat dan Manfaat Jahe Merah Meningkatkan Vitalitas. 17 september 2015 3:01 PM.

Sherwood, L. 2007. Human Physiology From Cells to System. 6th Edition. Thomson Brooks/cole. USA

Srivastava S., P. Desai, E. Coutinho, G.Govil. 2006. Mechanism of Action of L-Arginine on the Vitality of Spermatozoa is Primarily Through Increased Biosynthesis of Nitric Oxide. Tata Institute of Fundamental Research. India. Biology of Reproduction Journal. Volume 74: 954-58.

Zen, N Fauziah, Thaib Siti Hildani, 2009. Testosteron dan Kesehatan Pria : Majalah Andrologi Indonesia. No.31/Th.6/September.2009/ISSNO25-429X, pp:1191-7. (Indonesia).

Zhang, Shu-Yun., Ito, Yuki., Yamanoshita, Osamu et al. 2007. Permethrin May Disrupt Testosteron Biosynthesis via Mitochondrial Membrane Damage of Leydig Cells in Adult Male Mouse. Journal Endocrine Society.148 : 3941 – 394.


Article Metrics

Metrics Loading ...

Metrics powered by PLOS ALM


  • There are currently no refbacks.

Indexed by:


Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.
Based on a work at

stats View My Stats.